... (2000) Nucleotide-induced conformationalchanges in P-glycoprotein and in nucleotide binding site mutantsmonitored by trypsin sensitivity. Biochemistry 39, 4559–4568.22. Nagy,H.,Goda,K.,Arceci,R.,Cianfriglia,M.,Mechetner,E.&Szabo´Jr, ... ATP hydrolysis are generally interpreted in terms of ligand-induced ATPase activation and concomitant transitionsbetween substrate-binding and substrate-releasing conform-ational states. The consecutive ... competition (i.e. complete suppression of MM12.10 labeling after UIC2 binding) may be intrinsic tothe normal catalytic cycle, or, alternatively, the CsA-typedrugs may induce a special conformation...