Báo cáo y học: "A passport to neurotransmitter identity" potx

... differentiation of serotonergic neuronsIn regard to other types of neurons, developing sero to- nergic neurons, which secrete the neurotransmitter 5-hydroxytryptamine (5-HT, serotonin), express a ... operating in dopamine synthesis, release and re-uptake. Through systematic analysis of these regions they find that genes for tyrosine hydroxylase (TH, cat-2), GTP cyclo-hydrolase (GTPCH, cat-4), ... pathway may be coordinately activated by a single or limited number of transcription factors acting on shared cis-regulatory elements. This basic concept has been discussed for more than 30 years...

Báo cáo y học: " A Leu to Ile but not Leu to Val change at HIV-1 reverse transcriptase codon 74 in the background of K65R mutation leads to an increased processivity of K65R+L74I enzyme and a replication competent virus" pptx

... K65R+L74V RTmaybeduringreinitiation and not during processivesynthesis [5,37].The effect of compensatory mutations on viral replica-tion and RT has been previously analyzed by severallaboratories ... due to Tyr to Phe substitution in the YMDD motif of human immunodeficiency virustype-1 reverse transcriptase is compensated by Met to Val substitutionwithin the same motif. Biochemistry 1998, ... comparison to K65R+L74V viruses, we analyzed processivity of virion-ass ociated RTs containing K65R+L74V, and K65R+L74Imutations in in vitro processivity assays. RT lysates wereprepared by centrifuging...

Báo cáo y học: " A method to estimate the efficiency of gene expression from an integrated retroviral vector" potx

... puromycin selectedcells seeded was very low such that in theory only a smallnumber (2–5) of cells in the culture were puromycinresistant, the probability of proliferation was essentiallystochastic ... complete itslife cycle only if gene expression from the provirus occurs.Studying the efficiency of proviral gene expression canpotentially yield insights into the biology of this impor-tant ... as detected by direct PCR, especially atearly time points after transduction when using vectorsproduced by the calcium BBS transfection method. To avoid the detection of passively transferred...

Báo cáo y học: "A call to arms to reduce premature deaths by using inexpensive resuscitation care" potx

... citizens to emergencymedical services, delivery of care to the patient, delivery ofthe patient to the hospital, and delivery of cardiac and criticalcare once there. The time has come for us to come ... emer-gency crew-witnessed cases and bystander cardiopulmonaryresuscitation. Circulation 2008, 118:389-396.8. The Hypothermia after Cardiac Arrest Study Group: Mild thera-peutic hypothermia to improve ... randomized trial of a resynchronizationtherapy (2005-2009). He has received equipment, includingmannequins (Laerdal Medical, Stavanger, Norway) andmonitor/defibrillators (Physio-Control Inc., a...

Báo cáo y học: "A case of mistaken identity: Asystole causing convulsions identified using implantable loop recorder"

... MD, Director Electrophysiology Services, Division of Cardiology, Department of Medicine, Health Sciences Campus, University of Toledo Medical Center, Mail Stop 1118, 3000 Arlington Ave., Toledo ... convulsive activity thought to be due to epilepsy or conversion disorder. Key words: Asystole, implantable loop recorder, transient loss of consciousness Case description A thirty four year old woman ... Beverly Karabin, Yousuf Kanjwal, Blair P Grubb  Electrophysiology Section, Division of Cardiology. Department of Medicine, The University of Toledo Medical Center, Health Science Campus, Toledo...

Báo cáo y học: "The right to be wrong" potx

... American primary process. I also didn’t thinkanything I could say would have any connection to genomics. Yet, as I watched the campaigns for both partiesunfold, a connection did occur to me - one ... a connection did occur to me - one that seemed notonly to be ignored by most commentators but also to besurprisingly relevant. It has to do with the issue of ‘flip-flopping’ - of changing ... shows. (Of course, manyAmericans also believe that the Earth is 5,000 years old.Come to think of it, George W Bush is one of them.) To hold to your ideas when the facts show they are wrong isn’t...

Báo cáo y học: "Users' guides to the medical literature: how to use an article about mortality in a humanitarian emergency"

... they divided DRCongo into two strata along the 2001 line of military con-trol: an east stratum of territory formerly held by rebelgroups and a west stratum of territory formerly held bygovernment ... 5[36,37], the reliability of a mortality estimate can varywidely. If one were to rely only on the first estimate fromthat study, as many have, one would incorrectly infer that500,000 children ... continuum ranging from strong studies thatare very likely to provide an accurate estimate of mortality to weak studies that are very likely to yield a biased esti-mate of effect. In this case,...

Báo cáo y học: "A cyclic-RGD-BioShuttle functionalized with TMZ by DARinv “Click Chemistry” targeted to αvβ3 integrin for therapy"

... N-(2-Aminopropyl)-4-(6-(pyrimidine-2-yl)-1,2,4,5-tetrazine-3-yl)benzamide 4 4-(6-(Pyrimidine-2-yl)-1,4-dihydro-1,2,4,5-tetrazine-3-yl)benzoic acid (3) was prepared from 2-cyanopyrimidine 1 a nd 4 -cyano-ben ... standardized plan of treatment for every single patient and all available therapy options should be used, such as immunothe-rapy, surgery or chemotherapy sensibly using cytos-tatic active agents with ... -ligand, ligated to the cytotoxic drug TMZ. The ligation was carried out by the Diels Alder Reaction with inverse electron demand (DARinv). Key words: Click-Chemistry, Cycloaddition, BioShuttle,...

Báo cáo y học: " A Practical Approach to Managing Patients with HCV Infection"

... Sci. 2006, 3 65underlying autoimmune diseases, thyroid disorders, pregnancy, etc. In addition, the history can identify other factors such as alcohol use or abuse which may suggest possible ... strategies. 2. Natural History of HCV Infection The natural history of HV infection was thoroughly reviewed by Drs. Chen and Morgan in this issue. Knowing the natural history of HCV infection is ... non-specific symptoms such as fatigue, arthralgia, myalgia, anorexia weight loss and depression. It is unclear whether these symptoms can be solely attributed to HCV infection or whether they are...

Báo cáo y học: "A Practical Approach to Management of Chronic Hepatitis B"

... improved histology. The overall response to one year of ADV treatment as determined by HBeAg seroconversion is approximately 12% (vs. 6% in placebo group) that could be increased to 23% by the end ... sensitive to LAM treatment [16]. 4. Indications of HBV Treatment Historically, levels of HBV DNA and ALT, and histological activity of liver biopsy have been used as the three main factors to determine ... HBV is not directly pathogenic to hepatocytes and host immune response to HBV antigens expressed on the infected hepatocytes is the principle determinants of the liver injury. Thus, a high HBV...

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