... 1(3):221-6.28. Hasegawa K, Tamari M, Shao C, Shimizu M, Takahashi N, Mao XQ, Yamasaki A, Kamada F, Doi S, Fujiwara H, Miyatake A, Fujita K, Tamura G, Matsubara Y, Shirakawa T, Suzuki Y: Variations in the ... PAS-stained areawas electronically measured and the mucus index wasdetermined by the following formula: Mucus index =(PAS-stained area/bronquial cross-section area) × 100.Determination of airway ... This is accordance to Takedaand collaborators' observation that the intranasal instilla-tion alone of BtE elicited an IgE antibody response onlywhen the antigen was continuously administered...