... a-dystroglycan receptor affinity
of LCMV pseudotyped lentivirus yields unique cell and tissue tropism.
Genetic Vaccines and Therapy 2011 9:8.
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and take ... and Therapy 2011, 9:8
http://www.gvt-journal.com/content/9/1/8
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RESEARCH Open Access
Altering a-dystroglycan receptor affinity of LCMV
pse...
... percentage
of SGHPL-4 and HFF cells expressing IE 1/2 over a period of time. Representative images of HCMV IE 1/2 are depicted at 8 h
p.i in (B) CTBs and (C) HFFs and at 120 h p.i. in (D) CTBs and ... three fields of view were considered and the percent of IE-positive cells was calculated as: (average
number of IE-stained cells/average number of DAPI-stained cells) ×...
... formalin-fixed and paraffin-embedded tumor
tissue blocks were retrieved from the files of the Institute
of Pathology (University Hospital of Essen, Germany) and
processed using the tissue microarray ... pattern. One tissue core of each normal thyr-
oid and kidney tissues in preset position in each block
served as control tissue and helped with the orientation.
5 μm TMA...
... variable virus receptor affini-
ties [91,92], evolutionary adaptation of receptor affinity
[93], emergence of escape variants with altered receptor
affinities [94], temporal alteration of receptor ... both
non-inflammatory receptor blockade and immune-medi-
ated inflammation directed at viral protein -receptor com-
plexes could cause pathology of tissues non-permissive...
... distribution of apop-
totic cells or pyknotic cell clusters in the neuroepithelium of
Ptdsr
-/-
and Ptdsr
+/+
mice. Thus, reduced cell death or dimin-
ished clearance of apoptotic neural progenitor cells ... number of receptors have been iden-
tified that can mediate phagocytosis of apoptotic cells.
These include, for example, scavenger receptors and pattern
recognition rece...
... methylcellulose culture for PCR
analysis of the presence of the pIL3 plasmid.
DNA and RNA analyses
The simultaneous isolation of total cellular RNA and DNA
from tissues or cells was performed using TriPure ... adrenal glands, and
brain. PCR analysis of genomic DNA and RNA isolated
from bone marrow and blood (or serum) was performed
using primer specific pIL3 sequences. Speci...
... type I receptor specificity profiles of BMP-2,
BMP-6, and BMP-7, we used in vitro interaction analy-
sis (Table 1). Ligand proteins of BMP-2 (E. coli),
BMP-6 (CHO cells) and BMP-7 (NS0 cells) were
immobilized ... microgram of BMP-6, with a reac-
tion time of 8 h at 37 °C. For glycosylation trimming, a
mixture of 1 mU of PNGase F3 and 1 mU of PNGase H
was used per microgram...
... the surface of
virions and infected cells [23]. To gain further insight into
the structural properties of gp64 and to increase the
accessibility of peptide insertions to their ligands, we
inserted ... the
surface of infected cells was determined by FACS analysis
24 and 48 h.p.i. For this purpose Sf9 cells were infected
three times, independently with an m.o.i. of 10 using 6-we...
... United Kingdom
of Great Britain and Northern Ireland,
4
Tameside General Hospital, Ashton-under-Lyne, OL6 9RW, United Kingdom of Great Britain and Northern
Ireland and
5
Monklands Hospital, ... 8HE, United Kingdom of Great Britain and Northern Ireland,
2
Harrogate District Hospital, Harrogate,
North Yorkshire, HG2 7SX, United Kingdom of Great Britain and Northern Irelan...
... self-help groups and
PLHIV, and by HIV counsellors at the VCT centre of the
Impact theory of PEPPFigure 1
Impact theory of PEPP. PEPP was designed to promote community awareness, empowerment and participation ...
combination of activities, including participatory trainings for peer educators, outreach educational activities with distribution
of IEC materials, and referrals...