... 5¢-ATGTTAAAGAAAGTATTAAGTTGTTG-TATTGCAGC-3¢ and5¢-AAAGTTTGCTTGGAGCGTCAAGCC-ACTGTAAGCCG-3¢, cloned into the expression vector pBAD-TOPO,transformed into the E.coliTop10 strain and grown on LBagar ... cloning of the proteinase gene primerswere designed from the sequence of Vibrio alginolyticus [42]:5¢-GCGGAATTCTACACCCGCTACATGTGGCGTCGCCAT-3¢ and 5¢-CGCGGATCCTGGGGACTAGATCGAATC-GACCAACGTAA-3¢. ... 595–604.70. Matsuzawa, H., Tokugawa, K., Hamaoki, M., Mizoguchi, M.,Taguchi, H., Terada, I., Kwon, S.T. & Ohta, T. (1988) Purificationand characterization of aqualysin I (a thermophilic alkaline...