Báo cáo y học: "Analysis of genetic systems using experimental evolution and whole-genome sequencing" docx

... Hegreness and Kishony http://genomebiology.com/2007/8/1/210Genome Biology 2007, 8:201Genome Biology 2007, 8:201MinireviewAnalysis of genetic systems using experimental evolution and whole-genome ... Hegreness*† and Roy Kishony*‡Addresses: *Department of Systems Biology, Harvard Medical School, Longwood Avenue, Boston, MA 02115, USA, †Department of Organismic and Evolutionary Biology and ‡School ... comparative study of extant genomes has revolutionizedbiology, shedding light not only on evolution but also onphysiology, genetics and medicine. But the utility of comparisons among naturally evolved...

Báo cáo y học: "Analysis of alternative splicing with microarrays: successes and challenges" docx

... Genomewide analysis of mRNAprocessing in yeast using splicing-specific microarrays.Science 2002, 296:907-910.22. Yeakley JM, Fan JB, Doucet D, Luo L, Wickham E, Ye Z, Chee MS, FuXD: Profiling alternative ... sample of 153 exon-skipping events predicted by themicroarray analysis, 73 were successfully validated by RT-PCR and sequencing (a 48% validation rate). This initialstudy has made a very substantial ... Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095-1570, USA.Correspondence: Christopher Lee. E-mail: leec@mbi.ucla.eduAbstractRecently, DNA microarrays have...

Báo cáo y học: " Analysis of IL-12 p40 subunit gene and IFN-γ G5644A polymorphisms in Idiopathic Pulmonary Fibrosis" docx

... pulmo-nary fibrosis (IPF) is characterized by a predominance of gene expression for Th2-type regulatory cytokines. Thispattern is associated with high levels of IL-4 and IL-5 and a paucity of IFN-γ ... identical conditions and as previouslydescribed [26,27].Data AnalysisThe genotype frequencies, allele carriage frequency and allelic frequency were determined by direct counting and they were compared ... M, du BoisRM and Jeffery PK: Different cytokine profiles in cryptogenicfibrosing alveolitis and fibrosing alveolitis associated withsystemic sclerosis: a quantitative study of open lung biopsiesEur...

Báo cáo y học: "Role of Genetic Polymorphisms in Therapeutic Response to Anti-Asthma Therapy" pot

... therapy, focusing on a potential genetic clue to medication response.Key words: asthma, genetic polymorphismsOur understanding of the pathophysiology and treat-ment of asthma is quickly advancing. ... majority of studies indicate that the Gly16 SNP appears to carry thegreatest potential clinical impact. The wild genetic makeup of the sixteenth amino acid position of the b2receptor is Gly-Gly; ... returned to using as-neededalbuterol. By the end of the study, subjects who wereJohn Oppenheimer: Pulmonary and Allergy Associates, Summit, NJ;Department of Internal Medicine, New Jersey Medical...

Báo cáo y học: "Impact of methylphenidate formulation on treatment patterns and hospitalizations: a retrospective analysis" pptx

... Doyle A: Patterns of psychiatric comorbid-ity, cognition, and psychosocial functioning in adults withattention deficit hyperactivity disorder. Am J Psychiatry 1993,150:1792-1798.23. Ratey ... Consumer and Specialty Pharmaceuticals.Authors' contributionsJK and ML conceptualized and designed the study. MLhad primary responsibility for analysis of and interpreta-tion of data, as well ... Adolesc Psy-chiatry 2003, 42:1415-1423.35. Biederman J: Impact of co-morbidity in adults with attention-deficit/hyperactivity disorder. Journal of Clinical Psychiatry 2004,65:3-7.Annals of General...

Báo cáo y học: "Analysis of HLA DR, HLA DQ, C4A, FcγRIIa, FcγRIIIa, MBL, and IL-1Ra allelic variants in Caucasian systemic lupus erythematosus patients suggests an effect of the combined FcγRIIa R/R and IL-1Ra 2/2 genotypes on disease susceptibility" pptx

... University. Genetic analysesDNA was extracted by the salting-out method described byMiller and colleagues [18]. Analysis of genetic polymor-phism was predominantly performed by polymerase chainreaction ... previouslydescribed [20].Analysis of FcγRIIIa gene polymorphismThe analysis of the F/V polymorphism was performedessentially as previously described [21].MBL gene polymorphismVariants of MBL ... production of IL-1Ra [33], which provides a possibility for a pathogeneticmechanism concordant with the genetic interaction seen inour study. Analyses of disease phenotypes were beyondthe scope of...

Báo cáo y học: "Analysis of immunoglobulin light chain rearrangements in the salivary gland and blood of a patient with Sjögren’s syndrome" pptx

... analyses of the frequency of occurrence and mutations of the highly mutable motifs RGYW and WRCYrevealed that mutations in nonproductive VLrearrange-ments of B cells from the parotid gland ... nonproductive and productive repertoires of these donors exhibited a comparable usage of Vκ and Jκas well as Vλ and Jλ gene elements [10,11].Determination of Taq polymerase fidelity and thefrequency of ... an over-representation of expanded B cells using VκA27, VκA19, and Vλ1C, a posi-tive selection of mutations (especially within RGYW and WRCY sequences), and indications of selection against Rmutations...

Báo cáo y học: "Analysis of Fcγ receptor haplotypes in rheumatoid arthritis: FCGR3A remains a major susceptibility gene at this locus, with an additional contribution from FCGR3B" ppsx

... cytotoxic granule and cytokine release), antigen presen-tation and antibody-dependent cellular cytotoxicity [6]. Con-versely, FcγRIIb contains an inhibitory motif in the cytoplasmictail and ... the collection of clinical data and the recruitment of patients into the study.DS, JR and VK undertook some of the genotyping assays onDNA prepared in the laboratory of EAJ and RWO, who partic-ipated ... withexpectations. Allele and genotype frequencies were compared using 2 × 2 and 3 × 2 contingency tables, respectively. Nod-ules are only rarely present during the early stages of RA and their absence...

Báo cáo y học: "Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reacti" potx

... followed by 40 cycles of 95°C for1 minute and 60°C for 15 seconds, as recommended by themanufacturer (Applied Biosystems). Real-time data were ana-lysed by using the Sequence Detection Systems software,version ... 1Standard curves generated for each assay by ten fold serial dilutions of template,withreal-time data analysed by using the Sequence Detection Systems software, version 2.2.1 (Applied Biosystems).See ... variability by using end-stage OA, age- and weight-matched samples, and stringentRNA quality control. A relatively high degree of heterogeneity(large 95% CIs) was observed in the level of gene...

Báo cáo y học: "Analysis of bronchoalveolar lavage fluid proteome from systemic sclerosis patients with or without functional, clinical and radiological signs of lung fibrosis" pot

... cultural examination of common bacteria and fungi, and of direct acid-fast bacilli smears (Kinyoun method) and culturesfor mycobacteria, and for the microscopic examination of Pneumocystis jiroveci ... of pro-inflammatory cytokines, the influx of inflammatory cells, and fibrinogenesis, whereas the downregulation of GSTP,Cu–Zn SOD and cystatin SN could favor vascular and lung tis-sue injury through ... alterations of several cell effectors, including fibrob-lasts, endothelial cells, and T and B lymphocytes, are intimatelyinvolved in the development of the clinical and pathologicalmanifestations of...

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