... terminal deoxynucleotide transferase(Stratagene). The cDNA with the polyA head was amplifiedwith the primer pair, 21 9R and poly T.PCR was performed using rTth DNA polymerase XL(PE Applied Biosystems) ... Heinemeyer,T.,Wingender,E.,Reuter,I.,Hermjakob,H.,Kel, A. E., Kel, O.V., Ignatieva, E.V., Ananko, E .A. , Podkolodnaya,O .A. , Kolpakov, F .A. , Podkolodny, N.L. & Kolchanov, N .A. (1998) Databases on transcriptional regulation: TRANSFAC,TRRD, and ... primer pair 1Fand poly T. The 5¢ region of the mRNA was determined by5¢ RACE [9]. Brie y, cDNA was synthesized with primer 21 9R, and a Ôpoly A headÕ was created following incubationwith dATP and...