... filtered by purifying selection. In contrast, occa-sionally a SI+ genotype arises and, because they survive and are maintained at a higher rate in stably-infectedmosquito lineages than I+ genotype, ... starting Maximum Parsimony(MP) trees and these were then used as starting trees toestimate ML trees using a fixed setting with GTRMIX and then using the automatically determined setting on the ... collecting and processing conditions, whichcould have reduced virus stability, infectivity, and titer,resulting in the I+ phenotype. However, the finding wasreplicated in multiple years. I+...