... an analysis of Drosophila alcohol dehydrogenase genes [54], weestimate that Tc-proPO2 and Tc-proPO3 arose by gene dupli-cation approximately 0.6 million years ago. The phylogenetic analysis ... an iCycler ther-mal cycler (Bio-Rad, Hercules, CA, USA) with a profile of 95°C for 5 minutes followed by 40 cycles of 94°C for 20 s,60°C for 30 s, 72°C for 60 s and 78°C for 20 s [82]. SYBRgreen ... annotation of clip-domain SPs/SPHs, serpins, spätzles,SRs and others; Toll and Imd pathways. Jay Evans: RT-PCR analysis; GNBPs and PGRPs. Zhiqiang Lu: C-type lectins,galectins, TEPs and JAK/STAT...