... endosomes and be transported easily in the cytoplasm. Fourth, theyshould enter the nucleus easily. For transfection in vivo,thisis one of the most important hurdles. Finally, cytotoxity isanother ... problem. Only low or nontoxic deliverysystems could be used in humans. Therefore, we must testdifferent delivery systems on RDOs, and try to lower theircytotoxity and improve their efficiency.FUTURE ... alignment. The DNA strand isresponsible for the correction, while the RNA strand stabilizes the structure. The RDO designed for reacting with the sense strand is moreefficient than that for the antisense...