... recognition site
in the ABCB1 model, which is confirmed by both the ABCB1 X-ray crystal structure and the site-
directed mutagenesis studies. The ABCB1, ABCC4 and ABCC5 models display distinct ... ATP -binding cassette (ABC) transporters ABCB1, ABCC4 and ABCC5 are involved in
resistance to chemotherapeutic agents. Here we present molecular models of ABCB1, ABCC4 and
AB...
... 5-day cultures of 50 mL to 7-day cultures of 500 mL
resulted in a even lower expression when analyzed by SDS/
PAGE. Therefore, we focused on the purification and
study of the rBla g ... candidate for
clinical use in diagnosis of cockroach allergy and develop-
ment of new forms of immunotherapy.
Keywords: allergen; hypersensitivity; IgE antibody; asthma;
cockroach.
Sensitization ....
... proliferation of synoviocytes in the
synovial lining layer, gross infiltration of lymphocytes, and
migration of atypical lymphocytes with nuclear indentation
into the synovial fluid (SF) and/or synovial ... study indicates that HTLV-I infection could
modify the inflammatory activity of ordinary primary OA,
whereby direct activation by Tax protein might account for
the increased conc...
... to bind to Tyr757 of gp130, Tyr985
of the leptin receptor and Tyr401 of the erythropoietin
receptor, Tyr729 of the granulocyte colony-stimulating factor
(G-CSF) receptor, Tyr800 of the IL-12 ... forms of cytokine receptors that lack
intracellular domains can inhibit the action of cytokines by
simple competition for cytokine binding. Endocytosis of
receptors and proteasomal...
... acid
substitutions were chemically synthesized by a solid-phase
procedure described previously, using a model 430 peptide
synthesizer (Applied Biosystems, Foster City, CA, USA) and
purified by high-performance ... liquid chromatography [14].
Measurement of T-cell cytokines by ELISA
Quantitative measurement of cytokines was performed as
described previously [12]. In brief, spleens an...
... and
type I collagen α1 chain were synthesized by Invitrogen (Table
1). Data analysis was carried out using the Gene Amp 5700
Sequence Detector System software (Applied Biosystem,
Foster City, CA, ... result of biomechanical forces as
well as biological sources, such as cycles of hypoxia, the pres-
ence of reactive oxygen species, accumulation of advanced
glycation end products and t...
... days did not modify the membrane expression of
RANK-L as evaluated by cytofluorometry and did not stimulate
the release of detectable amounts of OPG in neutrophil super-
natants as measured by ... without any significant changes
from day 1 to day 3 (Figure 3). The expression of OPG mRNA
by neutrophils incubated in CM was not yet detectable on day
2 and appeared only after 3 days...
... cartilage, key compo-
nents of the skeletal system. Its metabolism is characterized by
two opposing processes: formation and resorption. Typically,
diseases of bone are characterized by an alteration ... Framingham Heart Study cohort [25]. As
part of a study of the inheritance of OA, selected participants
in the Offspring cohort were originally examined between
1992 and 1994. Me...
... presence of
rheumatoid factor of any of the immunoglobulin isotypes
tested (IgM, IgA, and IgG) was considered as positive. The
study was approved by the Ethical Committee of the University
of Gothenburg. ... signifi-
cantly lower than that of plasma (94 ± 24 versus 141 ± 32
mg/L, P = 0.0001) as detected by its ability to promote actin
filament assembly.
In vitro, pGSN can be c...
... active
immune system by blocking deposition of host immune
factors such as complement on pathogen surfaces,
thereby inhibiting detection by innate immunity path-
ways. In a complement deposition assay, BtE264 ... presence of this cluster
was confirmed by whole genome sequencing of a representative variant strain (B. thailandensis E555). Both whole-
genome microarray and multi-lo...