... sprea d of HIV- 1 by tetherin [48,49 ] mightbe due to differential methodology and analysis (flowcytometry detection of CA p24 and virus-derived eGFPexpression compared to flow cytometry detection ... inhibits HIV- 1 release by directlytethering virions to cells. Cell 2009, 139:499-511.7. Iwabu Y, Fujita H, Kinomoto M, Kaneko K, Ishizaka Y, Tanaka Y, Sata T,Tokunaga K: HIV- 1 accessory protein ... probably more physiologi-cally relevant, our system has limited the variables to tetherin and Vpu only and shows similar tetherin-mediated effects on transfer and transmissi on. Althoughgenerally...