... order to determine the affinity for Ca21and thecooperativity of Ca21-binding to p26olf, we next analyzedthe stoichiometry of Ca21-binding to p26olf by flow dialysisin the Tris buffer. Ca21-binding ... 2001Calcium-binding by p26olf, an S100-like protein in the frog olfactoryepitheliumNaofumi Miwa, Yukiko Shinmyo and Satoru KawamuraFrom the Department of Biology, Graduate School of Science, Osaka University, ... EF-handmotif was replaced by glutamine, and the mutants thus prepared werenamed as DEF-A, DEF-B, DEF-C and DEF-D. The p26olf-specificinsertions of four residues are shown by white bars in the upper...