... Westburn Lane, University of St Andrews, Fife, KY16 9TS, UK2Department of Immunology and Molecular Pathology, Windeyer Institute of Medical Science, 46 Cleveland St, University College London, London, ... acid to tyrosine at position 116)was performed using Stratagene Quickchange (Stratagene,La Jolla, USA) methodology. Transfectants were selected and maintained in 1 mg/ml G418 (Geneticin, Invitrogen, ... immunofluorescence microscopy cellswere fixed in 2% formaldehyde in PBS, blocked with 1% BSA in PFN containing 0.2% saponin, stained with antibodies in PFN with 0.2% saponin, and stained with FITC-labelled...