Báo cáo khoa học: "Kerbs von Lungren 6 antigen is a marker of alveolar inflammation but not of infection in patients with acute respiratory distress syndrome" docx

... 1Kerbs von Lungren 6 antigen levels in plasma and in bronchoalveolar lavage fluidKerbs von Lungren 6 antigen levels in plasma and in bronchoalveolar lavage fluid. Plasma and bronchoalveolar lavage ... pathology of ARDS consists of inflammation in the alveolar space, with a predominance of neutrophils. Analysis of bronchoalveolar lavage fluid (BALF)from patients with ARDS has shown increased ... ARDS but is not elevated in critically ill patients at risk of the disease.BALF KL -6 correlated with plasma KL -6 and was related to theseverity of inflammation. Alveolar infection does not seem...

Báo cáo khoa học: Saccharomyces cerevisiae a1,6-mannosyltransferase has a catalytic potential to transfer a second mannose molecule ppt

... 17921–17931. 16 Nakayama K, Nakanishi-Shindo Y, Tanaka A, Haga-Toda Y & Jigami Y (1997) Substrate speciﬁcity of alpha-1 ,6- mannosyltransferase that initiates N-linkedmannose outer chain elongation in Saccharomyces ... a1 ,6- mannosidase(derived from Xanthomonas manihotis, data not shown), indicating that the second mannose was not attached to the nonreducing terminus with a1 ,6- link-age, because the a1 ,6- mannosidase ... (5¢-TTCAAAAGCATAGTATCCATAGCTGAGTAAATACCACCTCTTG-3¢),and the pPICZaA-ScOCH1 as a template. The bothD18 8A mutant and wild-type proteins were expressed asmentioned above. After the culture supernatants...

Tài liệu Báo cáo khoa học: Hypoxia downregulates farnesoid X receptor via a hypoxia-inducible factor-independent but p38 mitogen-activated protein kinase-dependent pathway doc

... acid-binding protein [13]. Thus, it is clear that FXR is a key sensor for bile acids and has a central role in maintaining bile acid homeostasis.Bile acid homeostasis is the result of a balancebetween ... roles in maintaining bile acid homeostasis, andtheir imbalanced activation may cause impaired bileacid metabolism, leading to cholestasis-mediatedcirrhosis. In this study, we have demonstrated ... deg-radation domain; however, in contrast to HIF- 1a andHIF- 2a, HIF- 3a does not possess a hypoxia-inducibletransactivation domain, instead, having a novel C-ter-minus with additional uncharacterized...

Báo cáo khoa học: The Vps4 C-terminal helix is a critical determinant for assembly and ATPase activity and has elements conserved in other members of the meiotic clade of AAA ATPases pdf

... material is availableonline:Fig. S1. Sequence alignment of the C-terminal regions of Vps4, spastin, katanin and ﬁdgetin from a range of species.This material is available as part of the online articlefrom ... these AAA ATPases. Thusmany AAA ATPases function as protein disassemblymachines [25]. ATPase activity of Vps4 is critical fordisassembling the MVB sorting machinery, includingthe endosomal sorting ... mutantFig. 11. A C-terminal helix is a characteristic feature of meiotic clade AAA ATPases. Sequence alignments of some of the proteins listed in the PFAM database that contain the Vps4 C-terminal oligomerization...

Báo cáo khoa học: Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures potx

... was that in the absence of a substrate,product, or inhibitor, the catalytic domain of Lon mayadopt an inactive conformation. This is a lesson worthremembering. A practical approach to evaluatingprotein ... multiplicity and the negative in uence of radiationdamage.The meaningfulness of measured intensities can begauged by the average signal-to-noise ratio,<I ⁄ r(I)>. This measure is not always absolutely ... affectinterpretation of such data. This is not a primer of protein crystallography for non-crystallographers, but rather the musings of four structural biologists, active in various aspects of crystallography,...

Báo cáo khoa học: Insect cytokine, growth-blocking peptide, is a primary regulator of melanin-synthesis enzymes in armyworm larval cuticle pptx

... radioactivitywas counted in a liquid scintillation counter (Aloka LSC-5100, Tokyo, Japan).Cloning and sequence analysis of TH cDNATotal RNA was isolated from integuments of day 0 lastinstar larvae using ... 10813–108 16. 11 Noguchi H, Tsuzuki S, Tanaka K, Matsumoto H,Hiruma K & Hayakawa Y (2003) Isolation andRegulation of melanin synthesis in insect cuticle Y. Ninomiya and Y. Hayakawa17 76 FEBS ... Differ-ential involvement of calmodulin-dependent proteinkinase II-activated AP-1 and c-Jun N-terminal kinase-activated EGR-1 signaling pathway in tumor necrosisfactor-ƒ and lipopolysaccharide-induced...

Báo cáo khoa học: 17b-Hydroxysteroid dehydrogenase type 11 is a major peroxisome proliferator-activated receptor a-regulated gene in mouse intestine pdf

... functions. In this study, I examined the PPARa agonist-inducedproteins in the intestine, another important organ for lipidmetabolism e xpressing a fairly large amount of PPARa in mouse and human, ... RNAs reach their maximal levels i n a day in t he liver but not in intestine [13]. The levels of theirmRNAs in intestine slowly increase during a few days of feeding a diet containing Wy14 64 3. ... mRNA by Wy14 64 3 wasreproduced in the cultured hepatoma Fao cells (Fig. 5).Almost a m aximal level of induction was achieved in bothtissues within a day, making a sharp contrast to the c ases...

Báo cáo khoa học: EspB from enterohaemorrhagic Escherichia coli is a natively partially folded protein pptx

... drawn only for visual assistance and is not a mathematical ﬁt.Fig. 5. Urea unfolding of EspB at various pH values and at 20 °C. (A) The far-UV CD spectra obtained in the presence and absence of ... 745–751.10 Vlademir VC, Takahashi A, Yanagihara I, Akeda Y,Imura K, Kodama T, Kono G, Sato Y, Iida T &Honda T (2002) Cortactin is necessary for F-actinaccumulation in pedestal structure induced ... from amino acid sequence data.Anal Biochem 182, 319–3 26. 69 Hamada D, Kuroda Y, Kataoka M, Aimoto S,Yoshimura T & Goto Y (19 96) Role of heme axialligands in the conformational stability of...

Báo cáo khoa học: Human retinol dehydrogenase 13 (RDH13) is a mitochondrial short-chain dehydrogenase⁄reductase with a retinaldehyde reductase activity pdf

... RDH13–His6 wasraised at Alpha Diagnostics International Inc. (San Antonio,TX, USA).For western blot analysis of RDH13 expression, samples of human tissue obtained from the Anatomical Gift ... has a muchgreater catalytic efficiency as a reductase than as a dehydrogenase. The catalytic efficiency of RDH13 as a retinaldehyde reductase is significantly lower than that of a related protein ... towards all-trans-retinaldehyde andappeared to prefer NADPH to NADH as a cofactor,because the conversion of 5 lm all-trans-retinaldehyde in the presence of 1 mm NADPH was about 20-foldgreater...

Báo cáo khoa học: Myocyte enhancer factor 2 (MEF2) is a key modulator of the expression of the prothoracicotropic hormone gene in the silkworm, Bombyx mori ppt

... 55, 73– 86. 6 Kawakami A, Kataoka H, Oka T, Mizoguchi A, Kimura-Kawakami M, Adachi T, Iwami M, NagasawaH, Suzuki A & Ishizaki H (1990) Molecular cloning of the Bombyx mori prothoracicotropic ... andinverted into black and white images. An area adjacent to thearea of interest (A) and an area from an image lacking a spe-cimen (N) were scanned as the background signals. WhenPTPCs were not visible, ... N-termini and within an adjacent 29-amino-acidregion referred to as the MEF2 domain. The MADSbox is essential for DNA binding and dimerization,and the MEF2 domain plays an important role in DNA...

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