... to the relevant antigen in the future. Activated
CD4+ cells are highly susceptible to HIV-1 infection and
typically die quickly as a result of the cytopathic effects
either of the virus or of the ... through CG methyl-
ation by specific DNA methyltransferases that are directed
to the target by the methylation of lysine 9 of histone 3
(H3K9-Me). These findings have led to a mod...
... z, respectively. Hence the system of equations can be rewritten:
ds
dt
ypyss
de
dt
ys q yz p ye e
dy
dt
pys
=+ − +
()
−
=+ − −+
()
=+
1’
’
qqyzpye e y
dz
dt
yqq yz z
’
’,
++−++
()
=−+
()
−
⎧
⎨
⎪
⎪
⎪
⎪
⎪
⎩
⎪
⎪
⎪
⎪
⎪
(1)
where ... to this dynamical system is
Gseyz=
()
0000
,,, .
Notice that the equation related to the recovered individuals can be decoupled from...
... anticipated. These factors significantly
reduced the power of the study. Based on their findings, the
authors estimate that more than 100 hospitals would be
needed to show the 30% difference they sought. ...
seen from baseline to the study period for both groups
combined.
Conclusion
The MET system greatly increases emergency team calling,
but does not substantially affect...
... series
will probably be familiar, it is hoped that there will still be useful
lessons to be learned, for example the underlying assumptions
of a hypothesis test that were not fully appreciated, or ... some
previously unrecognised confusion between terms.
The first article, presented in this issue, covers the presentation
and summary of data. It is unlikely that the material covered by...
... the catalytic efficiency of the
enzyme, the cold adaptation of the enzyme could not be
attributed to the presence of the Gly cluster in this element.
The present study supports the idea that the ... Gly261 by Ala in mutant
G261A resulted in an enzyme with slightly lower stability.
On the other hand, in the double mutant G261A /Y2 69A the
additional replacement of Tyr269...
... guanidine hydro-
chloride (GdnHCl), urea or elevated temperatures, they
destroy the tertiary structure of proteins which results in the
loss of enzymatic activity. Regarding the secondary struc-
ture ... secondary structure only after the
disruption of the native tertiary structure of RNase A
(Figs 2–4). Interestingly, the preservation of the tertiary
structure coincides with t...
... angle almost typical for the 3
10
helix.
In this region of the polypeptide chain, the secondary
structure is characterized also by the O
i
H
N
i þ 3
hydrogen
bonds, found in all 40
DYANA
structures ... residue for the final family is
given in Fig. S1. Fig. 3A shows the family of 40 structures
and Table 1 contains a summary of statistical data indica-
ting the quality of the 40...
... catalytic
activity proceed essentially by the same mechanism. Indeed,
catalytic residues occupy the same position in the 3D
structures of the two enzymes. In addition, the p redicted
secondary structures ... function,
display only one polar cavity in the vicinity of the conserved
catalytic Asp re sidue.
Keywords: neurospora; P-ATPase; homology model; cavity;
H
+
.
The 3D struc...
... previously described
[9,15]. MALDI-TOF/MS was performed on a Perseptive
DE-RP (Applied Biosystem) using a-cyano-4-hydroxycin-
namic acid as matrix.
Lethality assay in mice
The in vivo toxicity of ... venoms and
become key molecular probes for the characterization of
these channels. They are usually small basic polypeptides
(between 30 and 70 amino acids), cross-linked by three or
four dis...
... Results from other coprecipitation studies supported
the yeast two-hybrid results with the exception of the weak
interaction observed between LEF-3 and IE1 with the yeast
two-hybrid system (Table ... of PCR products into the BglII
and PspAI sites of plasmid phsp70HAHisVI+ containing
the 5¢ terminal amino acid sequence RYPYDVPDYARA.
The sequence YPYDVPDYA constitutes the haemag...