Báo cáo y học: " Determination of the relative amounts of Gag and Pol proteins in foamy virus particles" doc
Báo cáo y học: " Determination of the relative amounts of Gag and Pol proteins in foamy virus particles" doc
... example of the determination of the relative amounts of Gag and Pol proteins in purified PFVFigure 3Representative example of the determination of the relative amounts of Gag and Pol proteins in ... intensities of the bands in the lanes with recombinant PFV proteins shown in Fig. 3 and amounts of protein loaded onto the gelFigure 4Relation of the intensities of the bands in the lanes with recombinant ... efficient incorpora-tion of pol polyprotein into foamy virus capsids. J Virol 2002,76:10069-10073.6. Flügel RM, Pfrepper KI: Proteolytic processing of foamy virus Gag and Pol proteins. Curr...
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Báo cáo y học: "What is the best site for central venous catheter insertion in critically ill patients" doc
... although the meta-analysis from Ruesch and colleagues [1] supports the use of the internal jugularapproach, the data vary widely across studies, accordingto the experience of the operators and to the ... [3]. Finally, in a recent prospective cohort study notincluded in the meta-analysis by Ruesch and coworkers, the rate of tip malposition was 12/661 (1.8%) with the internaljugular approach and ... at increased risk for pulmonary complications(severe emphysema, acute respiratory distress syndrome)were not included in the analysis – a fact that may explainthis surprising finding. In a...
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Báo cáo y học: " Candidate polyanion microbicides inhibit HIV-1 infection and dissemination pathways in human cervical explants" docx
... reduction in tissue viability.Inhibitory effect of polyanionic compounds against HSV-2 infection of epithelial cellsFigure 6Inhibitory effect of polyanionic compounds against HSV-2 infection of epithelial ... dem-onstrating an IC50 of 62.4 (± 2.9) μg/ml.Inhibitory effect of polyanionic compounds against HIV-1 infection of T-cellsFigure 1Inhibitory effect of polyanionic compounds against HIV-1 infection of ... participated in the design of the study, carried outanti-viral determinations in cellular and tissue models,determined the pro-inflammatory cytokine response in cervical tissue, completed any statistical...
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Báo cáo y học: "Relationship between alcohol use disorders, cortisol concentrations, and cytokine levels in patients with sepsis" docx
... by higher anti-inflammatory cytokine I L10 and lower ratio o f pro-inflammatory cytokines (that is,IL1b,IL6,andTNFa) to anti-inflammatory cytokine(that is, IL10). The results of this study ... worryor complain about your drinking?3. Do you ever feel guilty about your drinking?4. Do friends or relatives think you have a drinking problem?5. Are you unable to stop drinking when you want ... our study results and other studiesmay be explained by the timing of cytokine measure-ment (within 24 hours of admission) and the patientpopulation studied. Other studies have examined po...
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Báo cáo y học: "Foundation for the Community Control of Hereditary Diseases, Budapest, Hungary"
... 66.83 per 1000 informative offspring in the 1980s and within this, the total rate of major congenital abnormalities was 27.01 per 1000 informative offspring [1,2]. The causes of congenital ... the spectrum of congenital abnormalities evaluated, the period of study (only at birth or in early neonatal period or prenatal or the whole infant period are included), the completeness of ... frequently terminated if the fetus is severely affected. Thus, the rate of defects is calculated for informative offspring including (i) live born infants, (ii) stillborn fetuses, and (iii)...
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Báo cáo y học: "Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline"
... germline gene therapy. A major problem is the relatively low rate of transgene integration: in mice, the overall efficiency of transgenesis (taking into account embryo loss in vitro and in vivo) ... very profound implications for human germline gene therapy. Gene transfer into embryos (using pronuclear microinjection or RVVs) is inherently very costly and technically demanding, due in ... germline gene therapy will remain insufficiently safe, excessively inefficient and of inadequate clinical value to permit its use. The widespread availability and applicability of safe and effective...
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Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt
... agmatinase with the GfiAandCfiT substi-tutions in the coding and noncoding strands, respectively, and using the 5¢ and 3¢ primers mentioned above, the fulllength agmatinase cDNA coding for the ... separately alignedwith the sequences of 1CEV and 1RLA and pasted into the structural alignment. The alignment was then corrected byhand. Since the sequences of the two arginases and agmatinase ... connecting aminoacids at the beginning and the end, were mutated to glycines and, after remodelling back to their side chains in agma-tinase, the whole hydrogen bond network was optimized.The...
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Tài liệu Báo cáo Y học: Studies on the nonmevalonate pathway of terpene biosynthesis The role of 2C-methyl-D -erythritol 2,4-cyclodiphosphate in plants docx
... HA-hydrogen of IPP. In both cases, scrambling of the label takes place within the IPPpool and the error is then transcribed into the DMAPP pool by the normal action of the isomerase. The validity of the proposed ... Stereochemistry of the isoprenylation of tryptophan catalyzed by 4-(g,g-dimethylallyl) tryptophan synthasefrom Claviceps, the first pathway-specific enzyme in ergot alkaloidbiosynthesis. J. Am. ... q FEBS 2001into phytoene (the main labeled component of the lipid-soluble fraction) is systematically diminished by the addition of increasing amounts of [1-3H]2C-methyl-D-ery-thritol 4-phosphate....
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Báo cáo y học: "Antibodies against the CB10 fragment of type II collagen in rheumatoid arthritis" pdf
... wasused.Discussion The most significant finding from our study was that in RA the use in an ELISA of fragments of the intact CII molecule,particularly CB10, as antigen markedly increased the sen-sitivity of ... wasmarkedly increased, from 24% to 88%, comparing the fre-quency using the intact CII molecule; this was not at the expense of the specificity, which remained at the high level of 94%.Materials and ... However, this relatively low fre-quency in RA of anti-CII, together with seropositivity (albeitat even lower frequency) in other rheumatological and inflammatory diseases [7] including psoriatic...
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Báo cáo y học: "Longitudinal evaluation the pulmonary function of the pre and postoperative periods in the coronary artery bypass graft surgery of patients treated with a physiotherapy protocol" ppt
... with the goal of optimizing pul-monary function. Physiotherapy, including incentivespirometry, should be continued in the postoperativeperiod.Limitations of the studyThepresentstudyshouldbeinterpretedinthelightofsome ... supervised the study and contributed to the data analysis, manuscript writing and editing. All of the authors read and approved the final manuscript.Competing interests The authors declare that they ... evaluation the pulmonary function of the pre and postoperative periods in the coronary artery bypass graft surgery of patients treated with aphysiotherapy protocol. Journal of Cardiothoracic Surgery...
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