Báo cáo y học: " Effect of chloroquine on reducing HIV-1 replication in vitro and the DC-SIGN mediated transfer of virus to CD4+ T-lymphocytes" pdf
Báo cáo y học: " Chylothorax after surgery on congenital heart disease in newborns and infants – risk factors and efficacy of MCT-diet" pot
... lists the potential risk factors which have beenanalyzed in our database. Additionally we assessed the duration of drainage, the day of maximum loss of chy-lou s and the type and duration of treatment ... may appear due to injury of the thoracic duct, due to venous or lymphatic cong estion,central vein thrombosis, or diffuse injury of mediastinal lymphatic tissue in association with secondary ... first year of lifeonly the data of the last operation was used forinvestigation.Treatment of chylothorax On institutional consent, most patients with chylothoraxwere treated primarily with long...
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Báo cáo y học: "Rheumatoid peripheral blood phagocytes are primed for activation but have impaired Fc-mediated generation of reactive oxygen specie" pptx
... and examined with a haemocytometer within 5 minutes of the addi-tion of the dye. In addition to trypan blue staining, the apoptoticstate of cells was assessed by the determination of hypodip-loid DNA, ... and the remaining erythrocytes were lysed; the leucocytes were fixed with BD Fix and Lyse reagent (Bec-ton Dickinson). The cells were then washed twice more in staining buffer, resuspended to ... lessefficient in the induction of cross-linking, this becomes animpeding factor in the generation of ROS.Published data on neutrophil Fc -mediated ROS generationare conflicting. We demonstrated that the...
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Báo cáo y học: "Separating therapeutic efficacy from glucocorticoid side-effects in rodent arthritis using novel, liposomal delivery of dexamethasone phosphate: long-term suppression of arthritis facilitates interval treatment" pot
... corticosteroneproduction. Of note, the increased therapeutic potency of liposomal DXM-P resulted also in a significant reduction of urinary pyridinoline,indicating an inhibition of the ongoing, inflammation -mediated breakdown ... chains in these tissues are connected by 3-hydroxypyridinium crosslinks, which become discharged and excreted as pyridinoline upon disintegration of collagen [19].Levels of urinary pyridinoline ... hor-mone from the pituitary gland, leading to a suppression of cor-ticosterone production in the adrenal glands [20]. On the contrary, increased corticosterone levels are caused by the inflammation...
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Báo cáo y học: " Tumour necrosis factor-α stimulates HIV-1 replication in single-cycle infection of human term placental villi " docx
... titratedthem. FBS contributed to the design of the study and dis-cussion of the results. AA contributed to all the steps of the study and helped to draft the manuscript. EM conceived the study and ... Tremblay MJ: The low viral production in trophoblastic cells is due to a high endocytic internalization of the human immunodeficiency virus type 1 and can beovercome by the pro-inflammatory cytokines ... antigen of VSV-G pseudotyped HIV-1/ frag-ment, the increase of replication by TNF-α is onlyobserved at 120 h post-infection whatever the cytokineconcentration. At 2 ng of p24 antigen of VSV-G...
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Báo cáo y học: "Does intensive insulin therapy really reduce mortality in critically ill surgical patients? A reanalysis of meta-analytic data." pdf
... IntroductionTwo recent systematic reviews that evaluated intensive insulin therapy (IIT) in critically ill patients grouped the included randomized controlled trials (RCTs) by type of intensive ... meta-analytic results to methodologic deci-sions. In particular, the decision to group trials by type of ICU rather than by type of patient may not be intuitive for clinicians, for whom the important ... had no involvement in the conduct of this study.Author details1Interdepartmental Division of Critical Care and Department of Medicine, University of Toronto, R. Fraser Elliot Building, 3rd...
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Báo cáo y học: "A first city-wide early defibrillation project in a German city: 5-year results of the Bochum against sudden cardiac arrest study" potx
... precedingtraining and an estimation of the costs of the project in relation to the number of lives saved.MethodsBochum is a city of 380,000 inhabitants in the centralRuhr area. As a city-wide ... should be considered for more accu-rate time analyses.It must be mentioned that the training consisted mainly of one-time sessions, and follow-up training was onlyoffered to some extent. There ... planning and a clear strategic determination of the number of AEDs, AED locations and the groups of persons involved and to be trained. It was designed as an initiative sup-ported by different institutions...
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Báo cáo y học: " Parallel RNAi screens across different cell lines identify generic and cell type-specific regulators of actin organization and cell morphology" pptx
... lines is likely to reflect variation in the ease of identifying defects in cell mor-phology in each line, since all the phenotypes identified in the BG3-c1 cell line, which is prone to grow in ... cell lines, enabling us to look for correlations betweengene expression and function. Since the structural compo-nents of the cytoskeleton and their core regulators (for exam-ple, cofilin and ... sites of integrin adhesion to link the cytoskeleton to the plasma membrane. J Cell Biol 2001,152:1007-1018.13. Morrison DK, Murakami MS, Cleghon V: Protein kinases and phosphatases in the Drosophila...
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Báo cáo y học: "Parvovirus B19 Genotype Specific Amino Acid Substitution in NS1 Reduces the Protein’s Cytotoxicity in Culture"
... substitution of isoleucine 181 for methionine, as occurs in B19 genotype II, results in the reduction of B19 NS1-induced cytotoxicity of liver cells. Our results support the hypothesis that in the ... previously reported to be the mechanism of B19 NS1-induced cytotoxicity (34, 35). Further confirmation for the reduction of apop-tosis in the PathI transduced population of HepG2 cells was obtained ... apoptosis as the underlying mechanism for the cytotoxicity observed in the setting of transient B19 NS1 expression (34). We therefore examined whether the reduced cell loss ob-served in the...
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Báo cáo y học: "Histone deacetylase inhibitors modulate metalloproteinase gene expression in chondrocytes and block cartilage resorption" ppt
... least in part, by modifications to chromatin,including acetylation of histones. The aim of this study was to examine the impact of histone deacetylase (HDAC) inhibitors on the expression of metalloproteinase ... suppression of proin-flammatory cytokines such as TNF-α and IL-1 [56,57].All of these papers suggest a major effect of HDAC inhibitors in repressing the production of proinflammatory cytokines. ... repressed by the further addi-tion of HDAC inhibitors, a likely explanation is the ability of HDAC inhibitors to interfere with IL-1/OSM signalling. Sincethese cytokines are proinflammatory mediators,...
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Báo cáo y học: " High abundance synovial fluid proteome: distinct profiles in health and osteoarthritis" ppsx
... α1-Proteinase inhibitorApolipoprotein Aα1-Antitrypsin PEDF Fibronectin Tetranectin Fibronectin Clustrin H4 inhibitorα1-Antichymotrypsin Pregnancy zone proteinGelsolin α1-Acid glycoprotein ... inter-α-trypsin inhibitor, histidine-rich glycoprotein,gelsolin, vimentin, and numerous other protein species (Tables1 and 3) suggests contributions by these classes of protein to SF function. Further ... apoptosis by inhibi-tion of Bax [59]. In situ hybridization demonstratesupregulation of clusterin mRNA after exposure of chondro-cytes to oxidative stress, and may represent another pathwayby...
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