... alginolyticus [42]:5¢-GCGGAATTCTACACCCGCTACATGTGGCGTCGCCAT-3¢ and 5¢-CGCGGATCCTGGGGACTAGATCGAATC-GACCAACGTAA-3¢. Underlined are restrictionenzyme sites for EcoRI and BamHI, respectively. Theprimers ... d’Amico, D. & Gerday, C. (1999) Thermodynamicstability of a cold-active a-amylase from the Antarctic bacteriumAlteromonas haloplanktis. Biochemistry 38, 4613–4619.30. D’Amico, S., Gerday, C. ... Chessa, J.P. & Gerday, C. (2000) Structural, kinetic,and calorimetric characterization of the cold-active phosphogly-cerate kinase from the antarctic Pseudomonas sp. TACII18. J. Biol.Chem....