... Enzyme
inhibitors have a number of commercial applications as well. For example,
266
Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis.
Robert A. Copeland
Copyright
... inhibitors of enzymes that bind and are
released at rates that are rapid in comparison to the rate of enzyme turnover
and have overall dissociation constants that are lar...
... introduced to the common Lewis definition of
acids and bases: a L ewis acid is any substance that can act as an electron pair
acceptor, and a L ewis base is any substance that can act as an electron pair
donor. ... reactions. In the
Brønsted—Lowry classification, an acid is any substance that can donate a
proton, and a base is any substance that can accept a proton by reacting...
... Likewise, any ligand molecule must be
76
Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis.
Robert A. Copeland
Copyright
2000 by Wiley-VCH, Inc.
ISBNs: 0-4 7 1-3 592 9-7 (Hardback); ... same as for Figure 4.5.
in all fields put a great deal of emphasis on finding mathematical transform-
ations of data that would lead to linearized plot...
... Introduction to Structure, Mechanism, and Data Analysis.
Robert A. Copeland
Copyright
2000 by Wiley-VCH, Inc.
ISBNs: 0-4 7 1-3 592 9-7 (Hardback); 0-4 7 1-2 206 3-9 (Electronic)
5.5 THE SIGNIFICANCE OF
k
cat
AND
K
m
We ... of
EXPERIMENTAL MEASUREMENT OF k
cat
AND K
m
127
data points within this range. Table 5.1 illustrates an ideal situation for
determining...
... standard curve data
points must be collected at the same time as the experimental measurements,
however, since day -to- day variations in lamp intensity and other instrumental
factors can greatly ... referred to as the Stokes shift. For example, the amino acid
tryptophan absorbs light maximally at about 280 nm and fluoresces strongly
between 325 and 350 nm (Copeland, 1994). To take...
... integrated area under a peak in the chromatograph and compares
it to a calibration curve of the area under the peak as a function of mass for a
standard sample of the analyte of interest. Let us again ... ENZYME ACTIVITY
Figure 7. 18 Schematic diagram of a protease assay based on SDS-PAGE separation of the
protein substrate (20 kDa) and products (12 and 8 kDa) of the enzy...
... bimolecular reaction, similar to what we discussed in Chapters 8 and 9.
3 18
Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis.
Robert A. Copeland
Copyright
2000 by Wiley-VCH, ... Malcolm and Radda (1970) and Anderton and Rabin
(1970) for examples of this approach.
10.5.3.3 Affinity Labels. Covalent modifying groups can often be inco...
... End Station, Washington, DC 20037.
Telephone: 80 0-2 2 7-5 5 58.
387
Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis.
Robert A. Copeland
Copyright
2000 by Wiley-VCH, ... 48, 293.
Copeland, R. A. (1994) Methods for Protein Analysis: A Practical Guide to L aboratory Protocols,
Chapman & Hall, New York, pp. 151—160.
Copeland,...