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Báo cáo y học: "Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus" ppsx

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Báo cáo y học: "Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus" ppsx

... AccessResearchPhysiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirusBoe-Hyun Kim1, Harry C Meeker2, Hae-Young Shin1, ... immunocytochemistryFigure 3Confirmation of cell- type of established astroglial cell lines by immunocytochemistry. Characterization of cell types by immunofluorescence analysis. DAPI staining ... Densitometry of Akv gene expression levels in the ICR-A, R1A and P8A cell lines. Expressed levels of MuLV in P8A cell lines were significantly higher than in R1A cell lines. *statistically significant...
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Báo cáo y học: "APOBEC3G induces a hypermutation gradient: purifying selection at multiple steps during HIV-1 replication results in levels of G-to-A mutations that are high in DNA, intermediate in cellular viral RNA, and low in virion RNA" pps

... AWT BYAYBYCYDYEYFYGYHYIYJΔVif AΔVif B0500010000150002000025000300003500040000450005 7 9 11 13 15 17 19 21 23 25 27Counts/minuteCEM Round 2YBYA and YCWT P2YA P 2YB P ... used for HIV-YRHHY > A5. (E) Virus growth in Round 2 infections of non-permissive CEM cells. Virus from the peak of infection of HIV-YRHHY > A5 Round 1 sam-ples YA, YB, and YC and HIV WT ... thatallowed it to inhibit A3G and thus grow in the non-per-missive cells, 1000 RT unit aliquots of the HIV-YRHHY >A5 viruses from the days of peak RT for samples YA (day13), YB (day 11), and...
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Báo cáo y học: "A neurotropic herpesvirus infecting the gastropod, abalone, shares ancestry with oyster herpesvirus and a herpesvirus associated with the amphioxus genome" pps

... subunit of (putative) terminase and DNA polymerase. Conservation of aminoacid sequences in the terminase across all herpesviruses and phylogenetic analysis using the DNA polymerase and terminase ... molluscs, oyster and abalone, are distantlyrelated. The terminase and polymerase pro tein sequences from the putative amphioxus herpesvirus share moresequence similarity with those of the mollusc ... Terry A, Yu JK, et al: The amphioxusgenome and the evolution of the chordate karyotype. Nature 2008,453:1064-1071.16. Davison AJ: Channel catfish virus: a new type of herpesvirus. Virology1992,...
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Báo cáo y học" Increased plasma membrane cholesterol in cystic fibrosis cells correlates with CFTR genotype and depends on de novo cholesterol synthesis" pdf

... models of cystic fibrosis. Am J Physiol Lung Cell Mol Physiol 2007, 292:L476-486.4. White NM, Corey DA, Kelley TJ: Mechanistic similarities between cultured cell models of cystic fibrosis and ... accessibility compared to wild type controls. The hypothesis of this study is that membrane cholesterol accessibility correlates with CFTR genotype and is in part influenced by de novo cholesterol synthesis.Methods: ... BasalMedium (Biofluids Camarillo, CA) with 5% FBS. Mice Mice homozygous for the ΔF508 CFTR mutation weredescribed previously [12], as were mice carrying theR117H CFTR mutation [13]. Mice heterozygous...
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Báo cáo y học: " Altered intercellular communication in lung fibroblast cultures from patients with idiopathic pulmonary fibrosis" ppsx

... intrinsic ability of recipient cells to allow the spreading of the dye, butalso on the coupling of donor cells with recipient cells[52]. Ultimately, the time course depends on the number of active ... normal and fibrotic fibroblasts.Functional assay of gap junctional intercellular communicationFunctional assay of the gap junctional activity wasassessed with a dye-loading technique by means of ... by means of flowcytometry [29]. Lipophilic Dil stains plasma membranes of cells with a red fluorescence, while the membrane-per-meable calcein-AM is intracellularly hydrolyzed by non-specific...
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Báo cáo y học: " HIV-1 replication in cell lines harboring INI1/hSNF5 mutations" ppsx

... deletion of Ini1appears to induce apoptosis in lymphocytes in mice [47].On the contrary, cancer cell lines with INI1 deletion arecapable of survival presumably because of their specific cell of ... produced in MON cells fail to efficiently incorporate INI1. Immunoblot analysis of highly concentrated and subtilisin treated virions produced from 293T and MON cells in the presence of increasing ... produced in STA-WT1 cells effi-ciently incorporate INI1. Immunoblot analysis of highly concentrated and subtilisin treated virions produced from 293T and STA-WT1 cells in the presence of increasing...
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Báo cáo y học: "Macrophage migration inhibitory factor in cerebrospinal fluid from patients with central nervous system infection" potx

... with purulent meningitis of known aetiology or with encephalitis, and they were to some degree associated with severity of theinfection. Our findings indicate that MIF may play a pathophys-iological role ... (P < 0.05)but not with systemic inflammatory response (P > 0.05) inpatients with purulent meningitis of known aetiology, those with lymphocytic meningitis and those with encephalitis.Conclusions ... inhibitory effect of corticosteroids on the release of pro-inflammatory cytokines from lipopolysaccharide-stimulatedmonocytes. This suggests a role of MIF in both the initiation of and sustaining...
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Báo cáo khoa học: "Drotrecogin alfa (activated) should not be used in patients with severe sepsis and low risk for death" pdf

... the United States and European drug regulatory agencies approved DrotAA for use in adult patients with severe sepsis and high risk of death, as defined by an Acute Physiology and Chronic Health ... disease severity, type of organ dysfunction, and source of infection. There were no statistically significant differences in 28-day or hospital mortality between groups, but rates of serious bleeding ... manufacturer, Eli Lilly and Company, agreed to complete a number of phase IV studies evaluating the use of DrotAA in lower-risk patients with severe sepsis, in children with severe sepsis, and in patients...
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Tài liệu Báo cáo Y học: Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase from spinach leaves pdf

Tài liệu Báo cáo Y học: Kinetic properties of bifunctional 6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase from spinach leaves pdf

... inhibited b y Fru-6-P and Pi[1]. These properties allow the level of Fru-2,6-P2to respond sensi-tively to the availability of photosynthate and the accumu-lation of sucrose (the major photosynthetic ... theratio of 6PF2K activity to Fru-2,6-P2ase activity of thesmaller form of the bifunctional e nzyme was far lowerthan that of the larger form of the enzyme [11]. This isreminiscent of the enzyme ... core shares a high degree of sequenc eidentity with the corresponding region of the bifunctionalenzyme from other eukaryotic sources (Fig. 1). Notably,only f our of the known catalytic residues...
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Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc

Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc

... following atoms: Ct of the C-terminus, Cc of Asp and Cd of Glu, and Og of Tyr.Positive unit charges were added at the following atoms: Nt of the N-terminus, Nf of Lys, Cf of Arg, and ... very high pKavalue of Tyr16 can only beattributed to a highly desolvated environment, contributedby hydrophobic residues from both monomers. The rolethat Tyr16 plays in the structure of ... Waals model of the best structure of RIIa D/D. Thecentral part of this face appears to be highly charged with the presence of both negatively and positively chargedresidues, symmetrically arranged....
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