Báo cáo y học: "Contribution of the C-terminal region within the catalytic core domain of HIV-1 integrase to yeast lethality, chromatin binding and viral replication" doc
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Contribution of the C-terminal region within the catalytic core
domain of HIV-1 integrase to yeast lethality, chromatin binding and
viral replication
Zaikun Xu
†1
, Yingfeng Zheng
†1
, ... mutagenic
analyses of the C-terminal region of the catalytic core domain of HIV-1 IN in order to delineate the critical
amino...
... but ultimately tight, binding
of PLP to the apoenzyme in vitro and for the fact that
preparations of the mutant enzymes are always largely as
apoenzyme. Similarly, inflexibility of the protein ... Conversely, the less hydrated environ-
ment of the high-pH conformation favors the 340 nm-
absorbing enolimine tautomer of the wild-type cofactor
and the 388 nm-ab...
... histologically proved), non-compressive neuropathy
confirmed by electromyography, or the diagnosis of Sjögren
syndrome based on the clinical symptoms of dry eyes and dry
mouth (confirmed by a ... test and/ or kerato-
conjunctivitis sicca with involvement of salivary glands docu-
mented by positive lip biopsy and/ or salivary scintigraphy).
Simple x-rays of the hands and fe...
... of the protein. The position of lysines in two tri-
lysine regions and introduced mutations are shown at the bottom of sequence. B) The expression of the wild-type and mutant
IN-YFP fusion proteins ... of lysine residues and therefore, we term
them proximal tri-lysine region and distal tri-lysine
region, respectively (Fig. 2A). All of these lysine residues
ar...
... d b y
loose closure of the dura and skin layers presum a b l y
allows for expansion of the edematous brain into a
durotomy “bag” under the loosely closed scalp
without restriction by the ... meninges. The method
using temporal fascia is similar to the one introduced
by Yu et al.
32
They separated the temporal deep fascia
from the temporal muscle to the...
... score was
thirty-seven. Lifestyle Q1 to Lifestyle Q3 were equally
weighted and represented 81% of the total value of
the questions, whereas, Lifestyle Q4 and Lifestyle Q5
represented only ... CNS and psychiatric
conditions, and then to specify the name of that con-
dition. If the participant failed to indicate the name of
the condition regardless of a “...
... composition was very similar to the WSM, and
consequently the EDTA-IM. Hydroxyproline, hydroxy-
lysine and phosphoserine were not detected in WSM, WIM,
EDTA-SM and EDTA-IM.
The comparison of the amino ... Proline, hydroxyproline and
hydroxylysine were examined at 254 nm by reverse-phase
HPLC of their phenylisothiocarbamate derivatives [26], as
reported previously [20]....
... decades of life and severely impair visual
acuity in adulthood. Their therapy is restricted to keratopl-
asty and phototherapeutic keratectomy by Excimer laser.
Unfortunately, the benefits of these therapies ... that Cys–Cys bond
formation does facilitate amyloid precipitation, but is not
the only factor responsible for the amyloidogenicity of
C110)131 peptide. The particip...
... pathway to b-carot ene
proceeds via three enzymatic steps carried out by the
enzymes phytoene synthase, phytoene dehydrogenase and
lycopene cyclase. The enzyme phytoene dehydrogenase is
able to ... both
Zygomycetes. In Phycomyces they complement neither the
carR nor the carA mutants, and i n Mucor they complement
neither the carP nor the carR mutants. They are white, l ack...
... confirming the heterozygous sta-
tus (M/F) of the sample analysed.
Experimental strategy designed to analyse the new
a
s1
-Cas variant at the nucleotide level
To determine the coding sequence of a ... material to synthesize cDNA. However, as expected
from the phen otypic analysis (at the p rotein level), the f ew
animals yielding in their milk the a
s1
-CasM variant...