Báo cáo y học: "Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells" potx
Báo cáo y học: "Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells" potx
... very recent results sup port the notionthat epitopes derived from antisense transcripts s erve asCD8 T- cell targets in HIV-1 infection [21]. Takentogether, all these data s uggest that the HIV-1 ... CTCCA-CAATTAAAACTG-3’ (sense) and 5’-GTCCATGGCTG-TAATTCAACACAACTGTTTAATAGTAC-3’ (anti-sense). Primers permitting the addition of a Flag tag at the COOH end of the ASP ORF, 5’ -CATGGGACTACAClerc et ... nucleolus, a nd the mitochon-dria b ut not a t the plasma membrane [22]. The presence of ASP was de tect ed in the cytoplasm and the nucleus in the vicinity of the cell membranes. However, the nucleo-lar...
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Báo cáo y học: "Differential expression of the angiogenic Tie receptor family in arthritic and normal synovial tissue" doc
... CAG T CAT GCT AAG AAT TGA GTT AAT AAT AGG CTC GGT TCC CTT CCAng-2 CGC TCG AAT ACG ATG ACT CG TGC AGA GGC TGC AAG TGC TGG AGA A CCA CTG AGT GTT GTT TTC CAT GATTie1 GCC ACG TTC TGG CTG GAT TCA ... CTC CTC AGC TGT GGC AT ACT TCA CTT ACG CGG GCA TTTie2 GGC AAC TTG ACT TCG GTG CT ACT TAC ATC CCA GGG AGC AGT ACG TGG TC GGC CTT GGT GTT GAC TCT AGC T Arthritis Research Vol 4 No 3 Shahrara et ... at the mRNA and protein levels in RA synovial tissue compared to that from patients with OAand normal subjects. Hypothetically, in RA synovial tissue the dominating Ang-1 induces the Tie2 pathway,...
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Báo cáo y học: "Differential expression of the FAK family kinases in rheumatoid arthritis and osteoarthritis synovial tissues" doc
... involved in integrinαvβ3-mediated bone resorption [19,34-36]. Interestingly littleis known about the activation of these proteins in RA ST. In thisstudy we demonstrated the phosophorylation of FAK ... nonreceptor protein tyrosine kinases that are acti-vated by a variety of extracellular stimuli [1]. FAK and Pyk2associate with the cytoskeleton and with integrin-signalingcomplexes by binding to ... further activated pFAK, pPyk2 and pSrc expression up to120 min. The activation of the FAK-Src-paxillin-PLCγ pathway in RA ST lining and sublining suggests that these signalingproteins may be...
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Báo cáo y học: " Increased expression of matrix metalloproteinase-10, nerve growth factor and substance P in the painful degenerate intervertebral disc" pot
... specific activity than when activated byAPMA (4-aminophenylmercuric acetate), trypsin or plasmin[19,25,33]. Such potent activation of these proteins cantherefore shift the balance of activity in favour ... pathways, including analysis of enzyme activities, is required to better understand the underlying pathogenesis.Competing interests The authors declare that they have no competing interests.Authors' ... in symptomatic IVD degeneration, the pathwayappears to involve the induced or enhanced expression of both the neurotrophin NGF and MMP-10.Increases in IL-1 may both directly stimulate the expression...
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Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
... N-acetylation and the chymotrypsin-like activity of 20S proteasome. In the present s tudy, we detec ted the N-acetylation of OsRpt6 in the rice RP complex by MS. Other N-terminal modifica-tions ... them in both cytoplasm a nd nucleus of eukaryoteis ubiquitin-proteasome pathway that involves the covalentattachment of polyubiquitins to substrate targeted fordegradation. The ubiquitinated ... riceN-myristoyltransferase has a substrate specificity different from that of the other organism. Protein N-myristoylationpromotes reversible and weak protein membrane and protein protein interactions....
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Báo cáo Y học: Does phosphorylation of the cap-binding protein eIF4E play a role in translation initiation? ppt
... rate of protein synthesis in these studies. These data areconsistent with the notion that phosphorylation of eIF4Edoes not affect its interaction with eIF4G and indicates thatit is also insufficient ... pathway to stimulate protein synthesis. The authors argue that the inhibitory effect of Mnk activityon cap-dependent translation may act to limit translationunder certain physiological conditions, ... eIF4E in enhancing the export of the mRNA for cyclin D1 from the nucleus to the cytoplasm. The shuttling protein 4E -T [5] is thought totransfer eIF4E to the nucleus, where it appears to beimportant...
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Báo cáo y học: "4th meeting of the EU research network EUROME: From the identification of genes and cellular networks in murine models of arthritis to novel therapeutic intervention strategies in rheumatoid arthritis, London, UK, 9 March 2004" pot
... hypothesis was validated both in vitro and in vivo, in that arthritic fibroblasts exhibited F-actin stress fibres that were most probably due to the increased adhesion to the substratum (extracellularmatrix). ... severity of subsequent diseasecourse. After 2 years joint erosions are seen in most patients, and most patients become clinicallydisabled within 20 years. A recent meeting at the Kennedy Institute ... suchMeeting report4th meeting of the EU research network EUROME: From the identification of genes and cellular networks in murine models of arthritis to novel therapeutic interventionstrategies in...
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Báo cáo y học: " Antiretroviral activity of the aminothiol WR1065 against Human Immunodeficiency virus (HIV-1) in vitro and Simian Immunodeficiency virus (SIV) ex vivo" pptx
... Table 3, right column) suggests thatWR1065 did not inhibit the antiretroviral activity of AZT.On the contrary, combination of WR1065 with AZT didincrease the antiretroviral efficacy of AZT.Anti-SIV ... (apoptotic)and negativity (non-apoptotic).Flow Cytometry for determination of cell cycle parameters in human TCBs cultured in the presence of WR1065Flow cytometry was used to evaluate the integrity ... because they define the dose-response parameters and show consistency in HIV-1 inhibition for >20 experiments. In addition, in thisstudy the cytotoxicity was carefully defined in cell cycleTable...
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Báo cáo y học: "Mycotic aneurysm of the inferior gluteal artery caused by non-typhi Salmonella in a man infected with HIV: a case report" pot
... of the inferior glutealartery caused by NTS occurring in an adult Kenyanman infected with HIV. To the b est of o ur knowledge,this report represents the fir st of its kind in the medicalliterature.* ... with evidence of intra-luminal clot.His hemoglobin was 12.9 g/dl. The bloody aspirate,obtained prior to the administ ration of antibiotics, wassent for culture. He was admitted to our hospital ... isolate was sensitive to tetracycline, gen-tamicin and kanamycin and resistant to ampicillin,Figure 1 An ultrasound examination of his left buttockperformed on the day of presentation showing...
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Báo cáo y học: " Mycotic aneurysm of the inferior gluteal artery caused by non-typhi Salmonella in a man infected with HIV: a case report." pps
... distribut ion, and reproduction in any medium, provided the or iginal work is properly cited.rarity of aneurysms of the inferior gluteal artery, the lack of trauma, instrumentation, or another ... infection.ConsentWritten informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the writtenconsent is available for review by the Editor -in- Chief ... all patient data from the medical chart and laboratory records.PB wrote the sections relating to the surgical intervention and supplied two of the figures. All authors participated in the review...
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