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Báo cáo y học: "WFDC1 expression identifies memory CD4 Tlymphocytes rendered vulnerable to cell-cell HIV-1 transfer by promoting intercellular adhesive junctions" pptx

Báo cáo y học:

Báo cáo y học: "WFDC1 expression identifies memory CD4 Tlymphocytes rendered vulnerable to cell-cell HIV-1 transfer by promoting intercellular adhesive junctions" pptx

... al.: WFDC1 expression identifies memory CD4 T- lymphocytes rendered vulnerable to cell-cell HIV-1 transfer by promoting intercellular adhesive junctions. Retrovirology 2011 8:29.Submit your next ... Retrovirology 2011, 8:29http://www.retrovirology.com/content/8/1/29Page 13 of 14RESEARC H Open AccessWFDC1 expression identifies memory CD4 T-lymphocytes rendered vulnerable to cell-cell HIV-1 transfer ... [6,13-22]. More recently, our laboratoryidentified a novel HIV-1 enhancing pathway, namely thewhey acidic pro tein, ps20, in memory CD4 +Tlympho-cytes that promotes cell-free HIV-1 replication...
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Báo cáo y học: "Stable expression of a recombinant human antinucleosome antibody to investigate relationships between antibody sequence, binding properties, and pathogenicity" doc

... used to construct the supervectors by combining the light chain and heavy chain expression vectorsCloning method used to construct the supervectors by combining the light chain and heavy chain expression ... cases, expression was achieved usingheavy chain-loss variants, which are hybridoma cells that havelost the ability to secrete heavy chains. By transfecting suchvariants with expression vectors ... possibility.IntroductionSystemic lupus erythematosus (SLE) is an autoimmune rheu-matic disease of unknown aetiology, characterised by thepresence of autoantibodies against a multiplicity of nuclear,cytoplasmic,...
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Báo cáo y học: "Gene expression profiling in the synovium identifies a predictive signature of absence of response to adalimumab therapy in rheumatoid arthritis" doc

... Unfortunately, they did not perform any confirmatoryexperiment (real-time PCR or immunohistochemistry) in order to verify the reality of their microarray data [30]. Their resultswere also potentially ... wanted to increase the validity of suchmicroarray observations by performing additional RT-PCR andimmunohistochemistry experiments and by linking our data to potential mechanisms of resistance to ... early markers of response to TNF blockade. Genes significantly overexpressed at baseline inpoor responders are induced by several cytokines in FLSs,thereby suggesting a role for these cytokines...
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Báo cáo y học: " MicroRNA expression profiling of human breast cancer identifies new markers of tumor subtype" ppt

... thatbead-based flow cytometric miRNA expression profiling might be a suitable platform to classifybreast cancer into prognostic molecular subtypes.Published: 8 October 2007Genome Biology 2007, 8:R214 ... usingnative polyacrylamide gels, ensuring that only mature miR-NAs were assayed.Using this miRNA expression platform we analyzed a total of137 samples in 168 assays. Assays for 119 of these ... stability.Conclusion To date, many studies of miRNA expression in human cancerhave focused only on the deregulation of miRNA expression. Here we integrated the analysis of miRNA expression, mRNAexpression...
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Báo cáo y học: "ene expression analysis of human red blood cells"

... human erythrocyte proteome: analysis by ion trap mass spectrometry. Mollecular und Cellular Proteomics 2004;3:501-509. 9. Tyan YC, Jong SB, Liao JD et al. Proteomic profiling of eryth-rocyte proteins ... instance RNA polymerises I,II,III, zinc/PHD finger- DNA-binding proteins, cysteinyl, lysyl-tRNA syn-thetase), important RNA-stabilising factor - poly(A) binding protein, anti-apoptotic proteins ... contain typical eu-karyotic RNA with 28S- and18S-rRNA standard bands. Microarray studies revealed the presence of transcripts of 1019 different genes in erythrocytic RNA. Gene Ontology analysis...
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Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

Tài liệu Báo cáo Y học: Regulated expression and intracellular localization of cystatin F in human U937 cells pptx

... the cystatin F produced by U937 cells isretained intracellularly or is taken up by the cells immedi-ately following secretion. Accordingly, the relatively lowamount of cystatin F produced by ... thediglycosylated cystatin F species. The nature of this band ispresently unknown and is discussed below.In order to study the production of cystatin F compared to the ubiquitous inhibitor, cystatin ... F directlyin culture media or cell lysates by immunoblotting. How-ever, by absorption on Sepharose-bound Cm-papain fol-lowed by Western blot analysis, the cell lysate-derivedinhibitor could...
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Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt

Báo cáo Y học: Functional expression of human liver cytosolic b-glucosidase in Pichia pastoris Insights into its role in the metabolism of dietary glucosides ppt

... broadspeci®city w ith respect to the g lycone moiety of substrates.Human CBG hydrolyses synthetic aryl glycosides (including4-nitrophenyl and 4-methylumbelliferyl monoglycosides)[9], but no physiological ... 4NP-b-D-mannopyranoside (10 mM)].Hydrolysis of xenobiotic glycosides by reCBGThe a bility of CBG to hydrolyse a variety of glucosides wasassessed using a wide variety of aglycone structures ... activity towards 4NPGlc). Furthermore, reCBGhydrolysed various aryl-glycosides ef®ciently and wasinhibited in a time-dependent manner by 2,4-dinitrophe-nyl-2-¯uoro-2-deoxy-b-D-glucopyranoside...
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Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc

Báo cáo Y học: The expression of glutathione reductase in the male reproductive system of rats supports the enzymatic basis of glutathione function in spermatogenesis doc

... the total polyunsaturated fatty acids [9]. As polyunsat-urated fatty acids are vulnerable to peroxidation by ROS,and peroxidized lipids and carbonyl compounds produced by this reaction are toxic ... [28] andthen transferred onto Hybond-P (Amersham Pharmacia,Pscataway, NJ, U SA) under semi-dry conditions by meansof a Transfer- blot SD Semi-dry transfer cell ( Bio-Rad,Tokyo, Japan). The ... faldose reductase by catalyzing the formation of glutathioneconjugates.The enzymatic activity of GR in pachytene spermatocytesand round spermatotids is low compared to Sertoli cells,and the...
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Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx

Báo cáo Y học: Balanced expression of single subunits in a multisubunit protein, achieved by cell fusion of individual transfectants docx

... removedcompletely. The pellet was broken by gently tapping on thebottom of the tube and placed in a 37 °C water-bath.Prewarmed 0.5 mL of 50% poly(ethylene glycol) 1500(Boehringer Mannheim, Germany) was ... vectors whereeach gene are controlled by internal ribosome entry site(IRES) [9–11], panel of vectors [12] and multiple episomalvectors [13]. We chose secretory IgA (SIgA) as a modelprotein to ... distinctcells. Polymeric IgA (pIgA) is produced by B-cells afterassembly of light-, a- and joining (J)-chain [14]. SIgA issubsequently generated when a secretory component (SC)is covalently added to pIgA...
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Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt

Báo cáo Y học: Heterologous expression and folding analysis of a b-tubulin isotype from the Antarctic ciliate Euplotes focardii ppt

... still uncertainty about the amino acid at position 21encoded by a TAG codon, that may specify glutamine ortryptophan. Therefore, in order to subclone into the expression vector pET11a (NdeI/BamHI ... region) was automatically sequenced on anABI Prism sequence analyzer Model 373A (PE AppliedBiosystems) using the Big Dye Terminator Methodology(PE Applied Biosystems), to verify the correct ... focardiicytoplasmic extract preparation, SDS/PAGEand immunoblottingCytoplasmic extracts were obtained from E. focardii cellsdeciliated by vigorous shaking in 4% EtOH. Cell bodieswere recovered by...
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