... hans-ulrich.demuth@probiodrug.deAbbreviations: Fmoc-Ala-Pyrr-CN, 9H-fluorenyl-9-ylmethylN-[2-(2-cyano-1-pyrrolidinyl)-1-methyl-2-oxoethyl]carbamate;NHMec, 7-(4-methyl)coumarylamide; PEP, prolyl endopeptidase.Enzyme: prolyl endopeptidase ... are distinct from trypsin-type and subtilisin-type serine peptidases in their structure and the order of the catalytic triad residues in the primary sequence[2,3]. The recently reported three-dimensional ... standardprocedure [27]. PEP and actin were detected by the polyclonal antibody S449 (1 : 400 dilution) and monoclo-nal antibody anti-actin (1 : 2500 dilution, Sigma, A2066), respectively, and...