... assayed by flow
cytometry for cell surface expression of CD4, CXCR4 and
CCR5 (Figure 2). Potent downregulation of CD4 by Nef
was shown to occur, with cell surface levels being only
≈5% of those ... original work is properly cited.
Research
Expression of Nef from unintegrated HIV-1 DNA
downregulates cell surface CXCR4 and CCR5 on
T-lymphocyt...
... contains only one PAPP-A gene. Thus, PAPP-A
and PAPP-Ai mRNA likely results from alternative splicing
of a transcript from the same gene.
Expression and functional analysis of murine PAPP-A
and ... between human and murine PAPP-A.
Our cloning of cDNA encoding both PAPP-A and
PAPP-Ai allowed expression in mammalian cells and
functional analyses of the recombinant pr...
... inactivation study
Genomic DNA was extracted from 10 ml of peripheral blood
lymphocyte of patients and controls using standard methods
[16]. Genotyping of a polymorphic site in the androgen recep-
tor ... age-matched healthy women (n
= 257). XCI analysis was performed by enzymatic digestion of
DNA with a methylation sensitive enzyme (HpaII) followed by
PCR of a polymorphic C...
... study. By contrast, in
the COPE study patients only return ed to their usual
medication following an examination and decision by
one of the trial physicians. Thus, patients in WISP and
COSMIC may ... nly able to conduct one meta-analysis.
Meta-analysis
There are reasons for being cautious about the one
meta-analysis we conducted and, in general, meta-ana-
lysing results from these ty...
... gene expression
in adipocytes. It may be also speculated that the effect of
adrenergic system on mitochondrial biogenesis represents a
compensation for decreased ef®ciency of energy conversion
in ... insuf®cient supply o f ATP by mitochon-
drial oxidative phosphorylation [20].
Besides UCP1, ef®ciency of oxidative phosphorylation in
adipocytes may b e also c ontrolled b y r ecently...
... study the contribution of monocytes
overexpressing TLR-2 to the lung inflammation of COPD
patients and also the possible effect of lung environment
on the expression of TLR-2 after monocytes ... produced
by cells undergoing stress or necrosis [30,31]. Considering
that COPD is characterized by considerable tissue injury
Effect of methylprednisolone on the expression of TL...
... supernatant and inclusion body. Part of the production was applied to 12% SDS–PAGE analysis and
Western blot analysis. (A) SDS-PAGE analysis: Most of the fusion protein was found in inclusion body. The ... treatment of
type 2 diabetes [2].
Glucagon-like peptide 1 (GLP-1) is an incretin
hormone released from islet -cell and intestinal
L-cells in response to the ingestion...
... Negative controls consisted of omission of the
primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit
serum instead of the primary antibody.
Cell quantification ... is activated and incises the newly synthesized
DNA strand that contains the mutation [14]. Immunohistochemical study on non-polyposis colorectal
cancer demonstrated no e...
... hybridization. The nucleotide sequences of
plasmid DNAs were confirmed by restriction mapping and
by DNA sequencing.
In the cases of AACEVAPD3 and 6, one TAA codon in
their ORFs, thus should be converted ... [10]. The YN11 strain was derived from
YPH499 strain, and YN45 strain was prepared by the use of
YN11 and BJ5458 strains.
Preparation of
AACEVAPD1 –6
5
0
RACE and 3
0...
... a
comparative study in mice of UCP3 protein expression in
SS and IMF mitochondria of various muscle types. We also
examined UCP3 protein expression in the two mitochon-
dria populations of fed and fasted ... regulation of UCP3 protein expres-
sion may have important implications for the understanding
of its physiological role.
In conclusion, in this study, using a carefully...