Báo cáo y học: " Mechanism of HIV-1 Tat RNA translation and its activation by the Tat protein" ppsx
Báo cáo y học: " Mechanism of HIV-1 Tat RNA translation and its activation by the Tat protein" ppsx
... cloning of the Tat mRNA sequencesTo study the mechanism of protein synthesis from the Tat mRNA, we cloned the Tat1 and Tat2 sequences, the twomajor forms of Tat mRNA [43], by means of a PCR-DNAreconstruction ... of 18(page number not for citation purposes)RetrovirologyOpen AccessResearch Mechanism of HIV-1 Tat RNA translation and its activation by the Tat proteinNicolas Charnay1, Roland Ivanyi-Nagy1, ... slightly decreased the translation of the Rluc RNA and that of a 5' UTR -Tat RNA where the TAR-polyA has been deleted (Additional file 3). Thirdly, Tat synthesized in the RRL and the Tat/ RRL...
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Báo cáo Y học: Complexation of ytterbium to human transferrin and its uptake by K562 cells pot
... to the binding of the metal to the N-lobe,although the binding favorsthe C-lobe thermodynamically asjudged by 1H NMR. The more open conformation of the N-lobe may facilitate Yb3+ion binding ... ironrequirement and subsequently express much higher Tfreceptors [39]. Then the uptake of Yb-bound Tf by the tumorcells might be much higher than the normal cells and the uptake of iron by the tumor ... filter.RESULTSBinding of Yb3+to apo-hTf The thermodynamics of binding of Yb3+to apoTf.Binding of Yb3+to apo-Tf was investigated by addition of aliquots of Yb3+to a solution of apo-TF in 100...
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Báo cáo Y học: Interaction of bovine coagulation factor X and its glutamic-acid-containing fragments with phospholipid membranes A surface plasmon resonance study pdf
... bifunctional ligand, has been neglected. The consistency of the Kdvalues resulting from the evaluation of the equilibrium response signals and those obtained by evaluating the first step in the association ... previously [13,35]. The Kddetermined for factor Xis consistent with the value determined by McDonaldet al.[9]. The effect of the serine protease domain upon the membrane affinity of the intact ... for the Gla-domain as it is morenegatively charged. The stabilizing effect of EGFNon the structure of the Gla-domain is therefore even more than whatis reflected by the fivefold increase in the...
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Báo cáo y học: " Detection of HIV-1 RNA/DNA and CD4 mRNA in feces and urine from chronic HIV-1 infected subjects with and without anti-retroviral therapy" pptx
... diag-nosis of infection with human immunodeficiency virus type 1 (HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1 in urine, saliva, and/ orserum ... anindicator of subject's state of health[26]. HIV-1 RNA/ DNA and CD4 mRNA detected in the fecal specimens of HIV-1 infected subjects could be the result either from internalbleeding in the gastrointestinal ... in the fecal samples could be the result of the shedding of HIV-1 infected cells and/ or free virus from GALT into the intesti-nal lumen. HIV-1 has been detected in a variety of body fluids and secretions...
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Báo cáo y học: " Dynamics of HEV viremia, fecal shedding and its relationship with transaminases and antibody response in patients with sporadic acute hepatitis E" ppt
... when the ALT level was maximum.Clinical parameter and pregnancy The maximum duration of viremia was 42 days and 36days, fecal shedding 28 days and 19 days and IgM antiHEV 46 days and 49 days ... from the study because of lack of desired follow up. The mean age of the patients was 29.2 ± 8.92 years (range: 11 to 54 years) and there were 39 males and 21 females; there were 10pregnant and ... positivity of both IgManti HEV and HEV RNA. Positivity of various markers of HEV infection The positivity of IgM anti HEV was 78.3% at diagnosis and 18.3% at 7thweek, HEV RNA 86.7% at diagnosisand...
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Báo cáo y học: "Effects of cigarette smoke on degranulation and NO production by mast cells and epithelial cells" pot
... normalfunction of AEC by a variety of mechanisms. Some of these include decreases in the level of exhaled NO,enhanced release of pro-inflammatory cytokines, and inhibition of the airway repair process ... prostaglandins and a variety of cytokines [7]. In the lungs and skin of smokers mast cells increase in absolute numbers and smoking may be associated with activation of mast cells[8,9]. They may contribute ... IgG1 and IgG2a (DAKO) were used asnegative controls for iNOS and eNOS antibodies,respectively.Cell viability and cytotoxicityViability and cytotoxicity were assessed by trypan bluevital dye...
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Báo cáo y học: "Inhibition of HIV-1 replication in primary human monocytes by the IκB-αS32/36A repressor of NF-κB" docx
... lymphocytes and monocytes. We have previously shown thatIκB-αS32/36A, a proteolysis-resistant inhibitor of NF-κB, potently inhibits the growth of HIV-1 and SIVmac239 in cell cultures and in the ... vivo.Background HIV-1 infection is characterized by a long period of clini-cal latency followed by the development of acquiredimmunodeficiency syndrome, AIDS. During latency and when viral replication ... participated in the design of the study and discussion of results. IQ designed this study and edited the manuscript.Retrovirology 2004, 1:45 http://www.retrovirology.com/content/1/1/45Page 2 of 8(page...
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Báo cáo y học: "Expression of leukemia inhibitory factor (LIF) and its receptor gp190 in human liver and in cultured human liver myofibroblasts. Cloning of new isoforms of LIF mRNA" pdf
... isoforms of LIF mRNA. Expression of LIF by myofibroblasts and of its receptor by adjacent cells suggests a potential LIF paracrine loop in human liver that may play a role in the regulation of ... of endothelial cell and/ or hepatocyte labeling. Immunohistochemistry,flow cytometry and STAT-3 phosphorylation assays did not provide evidence for LIF receptorexpression by myofibroblasts themselves. ... Expression of LIF by myofibroblasts and of its receptor by adjacent cells suggest a potential LIFparacrine loop in human liver that may play a role in the regulation of intra-hepatic inflammation and...
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Báo cáo y học: " Influence of genetic variations in TLR4 and TIRAP/Mal on the course of sepsis and pneumonia and cytokine release: an observational study in three cohorts" pdf
... homozygous and TIRAP/Mal heterozygous. Of 29 TLR4 heterozygousmutation carriers, 24 were TIRAP/Mal heterozygous and 5 homozygous. The distribution of SNPs in the studiedpatients and the cohort of ... JMWvdM and KZ contributed to conception and design of the study. Statistical analysis was performed by OK and EJG-B.RRS headed the project, supervised and conducted the study. OK, EJG-B, AK and ... (Za243/8-1, 8-2 and 9-1). M.G.N. was supported by a Vidi Grant of the Netherlands Organization for Scientific Research. R.R.S. was supported by grants of the DFG and the Charité University Medical...
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Báo cáo y học: "Discovery of estrogen receptor α target genes and response elements in breast tumor cells" ppsx
... phenol-chloroform extraction steps as prescribed by the manufacturer.Microarray analysis of gene-expression profilesMicroarrays were generated by spotting the Compugen 19 Khuman oligo library, made by ... respectively, and hybridized to the arrays using protocols established by the Patrick O. Brown Laboratory [59]. Array images and datawere obtained and processed using the GenePix4000B scan-ner and ... pinpoint their lociwithin the genome. These annotations were chosen for theircomprehensiveness, in terms of number of annotated genes, and their consistency with the current state of NCBI contigdatabases....
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