Báo cáo y học: "Shockingly complex: the difficult road to introducing new ideas to critical care" doc

... scientist, there remain elusiveissues about the underlying pathophyiology of thesediseases, especially sepsis. An understanding of the CommentaryShockingly complex: the difficult road to introducing ... technologies with the potential to improve the clinician’s ability to monitor adequacyof ‘flow’ phase resuscitation. For advocates of the introduction of new diagnostic technology to the critical caresetting, ... ‘circulatory’ hypoxia [7]. What we donot know yet is whether therapy directed at any or all ofthese problems would either improve microvascular flow or,even if flow were to be improved, whether...

Báo cáo y học: "Gene Therapy: The Potential Applicability of Gene Transfer Technology to the Human Germline"

... and technically demanding, due in large part to the need to remove embryos from the female, to manipulate the embryos and finally to return the embryos to the reproductive tract. By contrast, ... Secondly, it could be the case that the trangene molecules bound only weakly to the sperm cells, such that only direct delivery (by ICSI) permitted the DNA to remain in place. If so, then this ... only if the efficiency turned out to be fortuitously high (i.e. similar to murine rates) would there be any point in attempting the technique with humans – but the necessary data on efficiency...

Báo cáo y học: "Evaluation of the proficiency of trained non-laboratory health staffs and laboratory" docx

... and delivery ward in our centrewithout any experience on laboratory works. The objective of this study was to assess the feasibility of the training by evaluating the proficiency of the trained ... centrifuged. The plasma samples werestored at 4 degrees centigrade and tested by the laboratorytechnicians with the same test kit on the next day. The testresults by laboratory technicians ... likely to occur in laboratory before giving reports to counsellors.Published: 20 May 2005AIDS Research and Therapy 2005, 2:5 doi:10.1186/1742-6405-2-5Received: 28 January 2005Accepted: 20 May...

Báo cáo y học: " Activation of the SPHK/S1P signalling pathway is coupled to muscarinic receptor-dependent regulation of peripheral airways" pot

... synthesis [4,5]. In the present study we therefore tested the hypothesis that acti-vation of the SPHK/S1P signalling pathway may contrib-ute to MR-dependent regulation of peripheral airwaydiameter. ... ofinhibiting DNA synthesis, whereas SPHK1 is mainly local-ized in the cytosol [14,16]. In the present study we there-fore focused exclusively on the expression and potentialfunctional role of the SPHK1 ... signaling pathways play a key role in the regulation ofairway resistance which is determined largely by the diam-eter of smaller, intrapulmonary airways [25]. A betterunderstanding of the different...

Báo cáo y học: " Optimization of the doxycycline-dependent simian immunodeficiency virus through in vitro evolution" docx

... even restore the preference for the EH structure. The only exceptions are the +46C-T and +72G-A mutationsobserved in culture 12, which only marginally affect the BH and EH stability. The virus ... up to 234 days. The period between infection and the appear-ance of syncytia decreased over time and we could reduce the volume of the virus inoculum that is needed to start a new infection. These ... pair by a G-U base pair). Since the equilibrium between the BH and EH conformers maybe essential in viral replication, we used MFold RNA anal-ysis to estimate the thermodynamic stability of the...

Báo cáo y học: "Analysis of the contribution of cellular and viral RNA to the packaging of APOBEC3G into HIV-1 virions" docx

... polyclonal antibody to the myc epitope tag (α-myc) or were mock immunoprecipitated (mock). Immunoprecipitated samples and total cell lysate (Total) were analyzed for the presence of APO3G by ... VB, Yonemoto W, Greene WC: Newly synthesizedAPOBEC3G is incorporated into HIV virions, inhibited byHIV RNA, and subsequently activated by RNase H. PLoSPathog 2007, 3:e15.21. Iwatani Y, Takeuchi ... of APO3G and notcaused by non-specific binding of the RNA to the mycantibody. Finally, the RT-PCR reaction was sensitive to treatment with RNase A as exemplified by the lack of 7SLRNA amplification...

... betweenmethemoglobin concentrations in patients and the presenceof hydrogen peroxide. The incident that they describe is by nomeans unique. Recently, the addition of silver-stabilisedhydrogen ... settingneed to be aware of any potential effects that disinfection of the water treatment system or the feed water may have on the product water used in the preparation of dialysis fluid. Suchawareness ... inwater to yield chlorite, chlorate, and chloride ions. Currently,there is little information about the potential for chlorinedioxide and its daughter products to be toxic to haemo-dialysis patients,...

Báo cáo y học: " Organization of the pronephric kidney revealed by large-scale gene expression mapping" docx

... in the final con-centration of urine [7] and is therefore the last structure of the nephron that can modify the electrolyte and fluid balance in the mammalian body. The constraints on the body's ... evolutionarily conserved down to the level of the pronephric kidney.Conclusion: The present study validates the Xenopus pronephros as a genuine model that may beused to elucidate the molecular ... X-tra-Solve (Medite Histotechnik, Burgdorf,Germany), rehydrated, and then treated according to the pro-tocol for fixation and acetylation of fresh frozen sections asreported by Yaylaoglu and coworkers...

Báo cáo Y học: Characterization of the self-splicing products of two complex Naegleria LSU rDNA group I introns containing homing endonuclease genes pdf

... polyadenylatedin vivo [14] a circular RNA could increase the stability, ortranslocation from the nucleus to the cytoplasm, prior to translation. Alternatively, full-length intron circles may ... Molecular phylogeny of the familyAnkist rode sma ceae (Chlorophy ta, Chlorophycea e) and ana lyse sof their group I intervening sequences, PhD Thesis, University ofErlangen, Germany.48. Decatur, ... analysedtogether with the corresponding DNA s equence marker. The DNAsequence is complementary to the RNA sequence shown in the lowerpanels. Processing sites are indicated by arrows. The internal...

Báo cáo y học: "Giantin is the major Golgi autoantigen in human anti-Golgi complex sera" pptx

... that are likely to extend from the Golgi membrane into the cytoplasm. Ourworking hypothesis is that aberrantly expressed Golgi complexautoantigens may be released into the immune system whencells ... study. The proportion of antibody to giantin was more than10-fold greater than that to golgin-97. To understand the mechanism of Golgi autoantibody pro-duction, it is important to consider why ... whencells undergo lysis. By virtue of a carboxyl-terminaltransmembrane domain, giantin is likely to be more stablyassociated with the cytoplasmic face of the Golgi complex thanare other golgins,...

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