Báo cáo khoa học: "Fertility of frozen-thawed stallion semen cannot be predicted by the currently used laboratory methods" ppt

... AccessResearchFertility of frozen-thawed stallion semen cannot be predicted by the currently used laboratory methodsP Kuisma1, M Andersson1, E Koskinen2 and T Katila*1Address: 1Department of Clinical ... predicting the fertilizing capacity of semen. The results of Exp. 1 could not be repeated, although the stallions and methods used were partly similar. Differ-ences in the outcome of these experiments ... trials of horses are bound to be of littlevalue because of these reasons [16].References1. Colenbrander B, Gadella BM, Stout TAE: The predictive value of semen analysis in the evaluation of stallion...

Báo cáo khoa học: Formation of highly toxic soluble amyloid beta oligomers by the molecular chaperone prefoldin pptx

... as there is no clearcorrelation between the number of amyloid plaque and the severity of dementia [2–5].It has recently been suggested that soluble Abspecies cause AD as the levels of these ... slows the oligomerization of Ab peptides by binding to the peptides in their oligomeric state.Figure 1B suggests that the number of PFD moleculesbinding to one Ab oligomer molecule is at the ... oligomerInhibit/Slow down oligomerizationAβ protofibrilFig. 8. Schematic model of the formation of soluble Ab oligomers in the presence of PFD.Formation of amyloid beta oligomers by prefoldin M. Sakono et al.5988...

Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc

... of ETA, or whether it involves the dual het-erogeneous part of the toxin. Use of this approach willprovide novel insight(s) into the physiological signiﬁ-cance of the endosomal fragments of ... (intrinsic apoptotic effect).Both pathways require the translocation of ETA into the cytoplasm of toxin-treated cells. On the basis of the reconstitution of the cytotoxic pathways within vitro cytosol ... interaction between ETA and cyto-solic EF-2 on the one hand, and the mitochondrialmembrane on the other hand. However, the potentialrole (if any) of ADP-ribosylation of EF-2 in the mitochondrial...

Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbersin parentheses below the diagram show the position of the ... detection software as the cycle number atwhich the ﬂuorescence of the reporter dye crossed the threshold in log-linear range of PCR. The copy numbers of the respective DNASE1 cDNA were quantiﬁed by ... and3¢ portion of 25 bp in intron 6 in fragments F and I. The number at the right of each RT-PCR product represents the length of the prod-uct. The scheme also shows the location of the reverse...

Tài liệu Báo cáo khoa học: Characterization of a recombinantly expressed proteinase K-like enzyme from a psychrotrophic Serratia sp. ppt

... gener-ated by clustalx [27] of some of these sequencesbelonging to the proteinase K family, and the number-ing in this alignment is used throughout the Resultsand Discussion. In addition to the mesophilic ... of SPRK shows > 43%sequence identity compared to the correspondingregion of the bacterial members in the PRK familycompared here (Fig. 2B). The only exception is the C-terminal region of ... shown to be essential for maintaining the activeconformation of VPRK, since cleavage of the disul-fides lead to inactivation of the enzyme [25]. The first two disulfide bonds in VPRK are the same...

Báo cáo khoa học: Control of transforming growth factor b signal transduction by small GTPases pot

... orthophosphate after the hydrolysis of GTP. Completion of the Ran cycle is shown in the middle for Smad3because the role of Ran has only been analyzed in detail in the case of Smad3. Cytoplasmic ... homology 1 domain of all Smads binds to importin-b in the case of Smad3and importin-a in the case of Smad4, while mutation of the NLS blocks the ability of these proteins to enter the nucleus [95–98]. ... Smad trafﬁcking – the role of the Ran GTPaseMost current evidence on the mechanisms that govern the dynamic shuttling of Smad proteins in the cell isbased on the behavior of engineered GFP–Smad2...

Báo cáo khóa học: Characterization of Mesorhizobium huakuii lipid A containing both D-galacturonic acid and phosphate residues ppt

... presence of GalA was unequivocally conﬁrmed by GC-MS analysis of trimethylsilil ethers of methyl glycosides liberated fromlipid A by methanolysis (Fig. 1). The 31P-NMR spectrum of the crude ... arecomponents of acyloxyacyl residues. The predicted ions for the third type of oxonium ions composed of DAG andtwo amide acyl residues have not been registered, due to the fact that the spectra ... isoccupied mainly by 3-hydroxyleicosanoic acids. The shor-ter acids were found to be bound at N-3 position of the amino sugar ring. The fatty acid distribution will be veriﬁed during further studies.Ó...

Báo cáo khoa học: Pleiotropy of leptin receptor signalling is deﬁned by distinct roles of the intracellular tyrosines docx

... cDNA of LEPRb,phosphorylation of STAT1, STAT3, STAT5 and the ERK kinases was induced by leptin. The weakresponse of STAT5 to leptin compared to that elicited by GH can be partially explained by ... Bahrenberg G, Behrmann I, Barthel A, Hekerman P,Heinrich PC, Joost HG & Becker W (2002) Identiﬁca-tion of the critical sequence elements in the cytoplasmicdomain of leptin receptor isoforms ... that the residual activation by the mutants retainingTyr1077 (YYF, FYF) can be explained by the action of STAT5. Spi2.1 promoter activity was stimulated by all constructs containing either Tyr1077...

Báo cáo khoa học: Characterization of a high-afﬁnity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus ppt

... France),quantiﬁed by the measurement of the peak area and referredto known quantities of pure peptides used as standards.Labeling of the toxin The labeling was carried out in 37 lL of 400 mMTris/HClbuffer ... and used within amonth after labeling.For iodination of the peptide using nonlabeled iodine, the protocol was as described above, except that 200 lgofthe3741 Da isoform, 0.3 mg of nonlabeled ... peptides, the absence of consensus residues in the activesite results in probable lack of any antiprotease activity [5].Then, the puriﬁcation of the binding site, and the cloning of the corresponding...

Báo cáo khoa học: Epoxidation of benzo[a]pyrene-7,8-dihydrodiol by human CYP1A1 in reconstituted membranes Effects of charge and nonbilayer phase propensity of the membrane pot

... affect the stereoselectivity of epoxidation. The presence of the anionic lipid PtdSerincreased the formation of DE2 twice as much as that of DE1. Even the relatively small portion of the negativelycharged ... stereoselectivity of epoxidation The stereoselectivity o f the e poxidation reaction can be demonstrated by the ratio of the formation rates o f the twodiol-epoxides, DE2 and DE1. These differ only in the conformation ... oxidation of t he(+)-7,8-diol was less pronounced.Effects of nonbilayer lipids on the metabolic rates of epoxidationWe analyzed the effects of a typical member of the nonbilayer c lass of phospholipids,...

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