Báo cáo khoa học: "Survival of Clostridium perfringens During Simulated Transport and Stability of Some Plasmid-borne Toxin Genes under Aerobic Conditions" pptx

... vet. scand. 2005, 46, 241-247.Acta vet. scand. vol. 46 no. 4, 2005Survival of Clostridium perfringens During Simulated Transport and Stability of Some Plasmid-borne Toxin Genes under Aerobic ... V: Survival of C. per-fringens during simulated transport and stability of some plasmid-borne toxin genes under aerobic conditions. Acta vet. scand. 2005, 46, 241-247. – Clostridium perfringens ... ob-jectives of this study were to investigate the survival of C. perfringens in a simulated transport of ﬁeld samples and to determine the stability of the plasmid-borne toxin genes cpb1 and etx...

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc

... Cu(I)4,Cu(I)4MT3 and Cu(I)6,Cu(I)6MT3]. The determination of a 3D struc-ture seems crucial for understanding the differencesbetween Cu–MT3 and Cu–MT1 ⁄ 2 (structure also notknown) and the presence and ... is a soft ligand and therefore shows a preference for soft metals. More-over the structures of metallothioneins are not rigid and hence there is little selectivity concerning the size of the ... Zn(II)-MT and Cd(II)-MT, apo-T is is oxidatively unstable under aerobic conditions asa result of the formation of disulﬁde bridges [2]. Sec-ond, in contrast to the high thermodynamic stability, the...

Tài liệu Báo cáo khoa học: Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica polymerase and silica esterase) doc

... development of a functional model that contrib-utes to understanding of the growth of the siliceousspicules which apparently lack a template or matrix.The simultaneous release of silicic acid and ... the rational application of silicatein inthe fabrication of (new) biomaterials based on layeredsilica, of titania and of zirconia [32]. This view is basedon the ﬁnding of a dual role for silicatein ... (Asn) and serine (Ser) [4,12]. In the active center of silicatein, thehydroxyl group of Ser26 and the imidazole of His165(catalytic diad) have been shown to play key roles inthe condensation of...

Tài liệu Báo cáo khoa học: Structural insights into the substrate speciﬁcity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... us to improve our understanding of the genericprinciple of catalysis for this family of proteases.Results and DiscussionFold of ervatamin-AThe overall structure and fold of ervatamin-A is ... R-factor of 19.78% and Rfree of 23.42%. The ﬁnal structure, after20 cycles of group B-factor reﬁnement using the same pro-gram, converged to an R-factor of 19.73% and Rfree of 23.38%. Data and ... [27], and thereforeonly its binding interactions with E-64 will be dis-cussed here.Modes of binding of E-64 with ervatamin-A and ervatamin-CThe modes of binding of E-64 with ervatamin-A and ervatamin-C...

Tài liệu Báo cáo khoa học: The role in the substrate speciﬁcity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx

... aglycone-binding site to the binding of diverse types of aglycones. Furthermore, these datamay be of particular importance in understanding thephysiological role of b-glycosidases and in designinginhibitors.In ... details about the role of different resi-dues of the aglycone-binding site in the stabilization of ESà and the interdependence between the binding of aglycone and the positioning of glycone in subsite ... binding of the second glucose unit of oligosaccharidic aglycones and also of the initial portion of alkyl-type aglycones. None of the residuesinvestigated interacted with the terminal portion of...

Tài liệu Báo cáo khoa học: "Collecting a Why-question corpus for development and evaluation of an automatic QA-system" pdf

... selection of natural questions. Thearticles varied in topic, degree of formality and theamount of details; from ”Horror ﬁlm” and ”Christ-mas worldwide” to ”G-Man (Half-Life)” and ”His-tory of London”. ... were grouped into groups of 5.Each HIT consisted of 5 assignments, and a $0.05reward was offered for each HIT.QRepHIT required the least amount of design and trials, and workers were delighted ... dynamic and inex-pensive method of collecting a corpus of questions and answers using the Amazon Mechanical Turkframework. We have provided to the communitya corpus of questions, answers and correspondingdocuments,...

Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

... ratio of 1. Asterisks and broken line: data and fitting with PHI/PHO ratio of 2.Fig. 5. Effect of PHI on the flavin fluorescence of the complex PHR–PHO. Dotted line: fluorescence emission spectrum of ... concentration of 0.6 lM. The data were fittedto Michaelis–Menten curves. Squares and dotted line: data and fittingwith PHI/PHO ratio of 0.5. Triangles and continuous line: data and fitting with ... firsthypothesis and therefore a direct involvement of PHI inelectron transfer. This conclusion is consistent with the verylow degree of identity between the N-terminal sequence of PHI and those of ferredoxin-like...

Báo cáo khóa học: Surface nucleolin participates in both the binding and endocytosis of lactoferrin in target cells potx

... site of the N-terminal lobe of human and bovine lactotransferrins. Importance of the glycanmoiety and of the non-covalent interactions between the N- and C-terminal lobes in the stability of the ... to this binding. Some of theseFig. 10. Prediction of nucleolin-binding sites in human lactoferrin (hLf).Sequences of the N2 and C2 domains of hLf, bovine lactoferrin (bLf) and human transferrin ... connected to bothinhibition of Cdk2 and Cdk4 activities and increase of Cdkinhibitor p21 expression [7]. Whether some of theseresponses are induced following interaction of Lf withnucleolin remains...

Báo cáo khoa học: Enzymatic toxins from snake venom: structural characterization and mechanism of catalysis ppt

... facilitate identiﬁca-tion of the key substrates of individual SVMPs and enable a better understanding of the molecular mecha-nism of action of P-III SVMPs.Functional role of the non-enzymaticproteinsIn ... Interac-tion of the cysteine-rich domain of snake venommetalloproteinases with the A1 domain of von Wille-brand factor promotes site-speciﬁc proteolysis of vonWillebrand factor and inhibition of von ... activity and toxicity of the basicsubunit, is a product of divergent evolution of the cat-alytically active and toxic PLA2. The salt bridgebetween Asp48 of the PLA2molecule and Lys60 of theacidic...

Báo cáo khoa học: End-damage-speciﬁc proteins facilitate recruitment or stability of X-ray cross-complementing protein 1 at the sites of DNA single-strand break repair docx

... species, during enzymatic processing of endo-genous DNA lesions, and as a result of aberrant DNAtopoisomerase I activity (reviewed in [1,2]). Many of the SSBs arise as a consequence of loss of the ... in assembly and stability of such complexes.Therefore, as recently reported, disruption of the inter-action of XRCC1 with PNK or Pol b leads to defici-ency in accumulation of PNK and Pol b in ... include identifica-tion of these lesions as a strand break, verification of the nature of the 3¢ end, and identification of a satis-factory pathway for repair. The XRCC1 component of the XRCC1–DNA ligase...

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