... Key Laboratory of Laboratory Medical Diagnostics, Ministry of Education, Department of Laboratory Medicine, Chong-qing Medical University, Chongqing 400016, China 2. Center for Hematology, ... [3, 4]. The most common molecular variant of the NPM1 gene is mutation A, accounting for about 75-85% of cases. It is due to a duplication of TCTG tetranucleotide at the C-terminus of the NPM1 ... given 2 days before harvesting the spleen cells. Experiments with injected mice were performed under the guidelines for care and use of experimental animals. 2.5 Cellular fusions When the anti-NPM-mA...