Báo cáo khoa học: "Characterisation of the Repeat Breeding Syndrome in Swedish Dairy Cattle" ppt
Báo cáo khoa học: "Characterisation of the Repeat Breeding Syndrome in Swedish Dairy Cattle" ppt
... lactation. In conclusion our results show that the repeat breeding syndrome is a multifactorial problem involving a number of extrinsicfactors as well as intrinsic factors coupled to the individual ... re-flected in the present study showing that ani-mals getting their first insemination during Jan-uary have higher risk of becoming RB animals. In agreement with our findings, Hewett (1968)found the ... frequency of RB is graphically exemplified in Fig. 1, show-ing that the frequency of RB is increasing by in- creased SCC and the increase is greater in larger herds than in smaller. As shown in Fig....
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báo cáo khoa học: " Characterisation of the legume SERK-NIK gene superfamily including splice variants: Implications for development and defence" ppt
... the truncation of the C-terminal domain in comparison toother SERK proteins. The actual function of the SERK C-terminal domain is unknown, but one possibility is a role in protein-protein interactions ... a predicted start codon in exon 9 in the regionencoding subdomain IV of the the kinase domain. Thissequence continues through to the position of the stopcodoninexon11ofSV1(usualSERKgenestructure).Thi ... truncated C-terminal domain, with the coding sequenceterminating just after the kinase domain (Figure 1).Expression of Medicago SERKs during the induction of somatic embryogenesis in culture The apparent...
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báo cáo khoa học: " Characterisation of the tryptophan synthase alpha subunit in maize" pptx
... whether the biosynthesis of aromatic amino acids is also partially active in the cyto-plasm, as e.g. a cytoplasmic isoform of chorismate mutaseis expressed in Arabidopsis [34]. As recombinant ... cloned into the SmaI/XbaI sites of the PvuII-deletion of pPCVE35E planttransformation vector. The same strategy was used for the construction of the ZmTSAlike-eGFP chimeric cDNA, with the primer ... Site-specificmutagenesis of the alpha subunit of tryptophan synthasefrom Salmonella typhimurium. Changing arginine 179 toleucine alters the reciprocal transmission of substrate-induced conformational...
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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
... motifArg40Cys84Tyr349Tyr347Lys370Ala36Ala36Ala36Val357Phe518BLaccaseTyrosinaseβ-sheet proteinFig. 1. Overview of the tyrosinase geneand surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinasegene and those in its immediate vicinity in the V. spinosum genome. ... transcript, indica-tive of a site of transcription termination. The presence of these features may indicate that the tyrosinase geneis part of an operon.As stated in the Introduction, V. spinosum ... Determination of the mass of the proteins byMS revealed masses of 36 506 kDa (recombinant coretyrosinase) and 37 874 kDa (trypsinized pro-tyrosi-nase) corresponding to a C-terminal amino acid of Val357...
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Báo cáo khoa học: Mapping of the functional phosphate groups in the catalytic core of deoxyribozyme 10–23 potx
... the P5 phosphate of adenosine at position 5, and the oxy-gen atom of the 6-carbonyl group of the subsequentnucleoside (G6). Our model of the metal-binding site in the catalytic core of deoxyribozyme ... s, making kinetic analysisimpossible. Whereas the P8 substitution had only aminor effect both in the presence of Mg2+and in the presence of Mn2+, causing a 30–60% increase in krel, the ... Mn2+resulted in a restoration of the activity to the level of the kMnobs of the unmodifiedenzyme (Table 1, Fig. 2B), implying a possible coordi-nation of the metal cation to one of the two (the proRPor...
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Báo cáo khoa học: Distribution of the lipolysis stimulated receptor in adult and embryonic murine tissues and lethality of LSR–/– pdf
... Itwould be interesting to determine whether it is suckling, like in the case of LRP [21], or weaning, as in the case of LDLR[24], which triggers the increase of LSR seen in liver, as suchan induction ... sections of livers of E14.5 em-bryos stained with hematoxylin and eosin(C,D,E,F). Note the presence of large inter-cellular spaces (arrow) in the liver of the mutant (C,E) contrasting with the ... elevation in the plasma concentra-tion of cholesterol and TG that were carried in apo B48-containing lipoproteins resembling remnants. Nevertheless, in LDLR-deficient mice the increase in TG levels...
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Báo cáo khoa học: Secretion of the mammalian Sec14p-like phosphoinositide-binding p45 protein pptx
... membrane and in the extracellular space. The weak staining of extracellular material is not surprisinggiven that detergents are included in the staining pro-tocol. Thus, the staining for PtdIns(3,4,5)P3is ... the SEC14 domain of PTP-MEG2 was shown to bind PtdIns(3,4,5)P3 in vitro andcolocalized with it in intact cells [5]. We therefore deci-ded to study the binding of the SEC14-containing p45to ... Expression of the p45 protein in COS-1 cellsresulted in accumulation of the protein in secretory vesicles and in the extracellular space. The secreted material contained PtdIns(3,4,5)P3. Ourfindings...
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Báo cáo khoa học: Proteolysis of the tumour suppressor hDlg in response to osmotic stress is mediated by caspases and independent of phosphorylation pot
... decrease in the levels of hDlg protein in the human epithelial cell line HeLa whenexposed to osmotic shock. This is independent of the phosphorylation state of hDlg, is prevented by preincubating the ... diffuselythroughout the cytoplasm in the vicinity of the mem-brane (Fig. 6Ca–c). However, pretreatment of the cellwith the p38MAPK inhibitor BIRB0796 causes a sig-nificant delay in the loss of Dlg from the ... itsdissociation from the cytoskeletal protein GKAP,therefore releasing it from the cytoskeleton into the cytoplasm [3].Our aim in this study was to gain a better under-standing of the role of hDlg phosphorylation...
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Báo cáo khoa học: Mechanism of the ring contraction process in vitamin B12 biosynthesis by the anaerobe Propionibacterium shermanii under aerobic conditions docx
... anaerobically cultivated in phosphateFig. 2. The mechanisms of the ring contraction process in the biosynthesis of vitamin B12postulated on the basis of the isolation of factor IVfrom the anaerobe P. ... the migra-tion of ring D followed by cleavage of the carbon–oxy-gen bond of the c -lactone at C1 of 13C,18O-precorrin-3x [8–10] as shown in the lower row of Fig. 1.We were interested in ... the lower row of Fig. 1. Therefore, examination of the 13C18O-carbonyl (C27) oxygen of the ring A acetategroup in 13C,18O-vitamin B12biosynthesized in [1-13C,1,1,4-18O3]ALA feeding experiments...
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Báo cáo khoa học: Activity of the plant peptide aglycin in mammalian systems potx
... emphasized that even though aglycin, likeinsulin, in uences the concentration of glucose in blood, the effect is opposite that of insulin, increasingrather than decreasing the blood glucose concentration.VDAC-1 ... aglycin binding protein purifiedby affinity chromatography. Lane A, protein molecular mass mark-ers; lane B, the aglycin binding protein isolated from mice; lane C, the aglycin binding protein ... [12,14,15].Due to the similarity with animal insulin in binding the basic 7S globulin and stimulating its protein kinaseactivity [16], the 4 kDa soybean peptide was initiallydesignated leginsulin [12]...
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