Báo cáo khoa học: "Plasma Melatonin Levels in Relation to the LightDark Cycle and Parental Background in Domestic Pig" ppt
Báo cáo khoa học: "Plasma Melatonin Levels in Relation to the LightDark Cycle and Parental Background in Domestic Pig" ppt
... p<0.001Andersson H: Plasma melatonin levels in relation to the light-dark cycle and parental background in domestic pigs. Acta vet. scand. 2001, 42, 287-294. – To study porcine melatonin secretion in ... genetic background. genetic.Acta vet. scand. 2001, 42, 287-294.Acta vet. scand. vol. 42 no. 2, 2001Plasma Melatonin Levels in Relation to the Light-Dark Cycle and Parental Background in Domestic PigsBy ... no importance for the role of mela-tonin as an endocrine signal of darkness. How-ever, increased diurnal levels of the mainurinary melatonin metabolite (6-sulfatoxy- melatonin) have been observed...
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báo cáo khoa học: "Trichoderma viride cellulase induces resistance to the antibiotic pore-forming peptide alamethicin associated with changes in the plasma membrane lipid composition of tobacco BY-2 cells" potx
... exposed to T. viride, and the situation is far from the soil situation wherefungus and plant grow together and influence eachother. The objective of the present investigation was to investigate ... cellulase and or macerozyme was inac-tivated by boiling prior to addit ion. In other cases, cellswere incubated in CM medium for 20 min and thenpelleted and transferred to Control medium and incu-bated ... to H2O2being a putative inter-mediate in the cellulase- initiated signalling cascade.Instea d, the presence of H2O2can lead to rapid cross-linking of the cell wall proteins [53]. The...
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Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... TK1. In the first crystalstructure of TK1-type enzymes of human and myco-plasmic origin [14], the feedback inhibitor dTTP wasbound in the substrate pocket, similar to the bindingof dTTP to the ... triphosphate to the 5¢-hydroxyl group of thymidine, forming dTMP. Human TK1 iscytosolic and its activity is absent in resting cells, appears in late G1,increases in S phase coinciding with the increase ... willresult in the apparent negative co-operative behavior. To further elucidate this, the relationship between the oligomerization status and the kinetic behaviour wasinvestigated, i.e. whether the...
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Báo cáo khoa học: NMR investigations of subunit c of the ATP synthase from Propionigenium modestum in chloroform/methanol/water (4 : 4 : 1) pot
... subunit c isdue to the interaction of the monomer with the artificialenvironment lacking the stabilizing protein/protein contactswithin the ring. The undecameric c ring of the ATP synthasefrom ... structure in the cystoplasmicloop region was not apparent, and neither was there a break in the a helix after the sodium ion-binding Glu65 residue. The folding of subunit c of P. modestum in the organicsolvent ... table, and resist boiling in SDS for 5 min in the case of the undecameric c ring of P. modestum [41]. This stability of the ring makes structural flexibility rather unlikely, indicatingthat the...
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Báo cáo khoa học: Treatment with small interfering RNA affects the microRNA pathway and causes unspecific defects in zebrafish embryos doc
... the enzyme responsible for the lastprocessing step of miRNAs, siRNAs are known to beassembled into effector complexes (siRISCs) containingArgonaute proteins as well as Dicer and other factors[3]. ... pre-miRNA processing by siRNAs competing for binding to Dicer, TRBP, and ⁄ or other limiting factors. How-ever, since our northern blot analysis did not reveal aconcomitant increase of pre-miRNAs, ... (pre-miRNA) is then exported to the cytoplasm by a nuclear transport receptor complex,exportin-5 ⁄ RanGTP [7,8]. In the cytoplasm, a secondRNase III, Dicer, cleaves the pre-miRNA to generate the mature...
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Báo cáo khoa học: Protective effect of active oxygen scavengers on protein degradation and photochemical function in photosystem I submembrane fractions during light stress pdf
... to obtain a better insight into the photo-inducedchanges in PSI. The submembrane particles used con-tain all the components of PSI, including the cyto-chrome b6 ⁄ f complex and plastocyanin ... at chilling temper-ature, photoinhibition of PSI coincided with the damage of both reaction-center proteins, PsaA and PsaB, and the proteins PsaD and PsaE on the acceptorside of the photosystem ... goodthing: light can be bad for photosynthesis. TrendsBiochem Sci 17, 61–66.7 Satoh K (1970) Mechanism of photoinactivation in photosynthetic systems. I. The dark reaction in photo-inactivation....
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Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt
... insulin 1 and 2 (b) and C-peptides1 and 2 (c) signals. The most abundant pep-tides turned out to be those from proinsulin.Insulin 1and2 andC-peptide 1and2 wereidentified, while the other prominent ... fragmentation In order to detect less dominating peptides and to verify the identities of the components assigned by mass accuracy in the direct infusion experiment, on-line LC was applied in combination ... spectrumoriginated from products of proinsulin. Two insulin variantsare expressed in mouse, insulin 1 and 2 [30]. Masses cor-responding to these two peptides were detected (Fig. 1b). The masses of the...
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Báo cáo khoa học: Complement factor 5a receptor chimeras reveal the importance of lipid-facing residues in transport competence doc
... no detectable binding. To determine whether the decrease in binding affinity in the other chimeraswas the result of protein instability and misfolding orwas merely a byproduct of the localization ... inter-action of the calcitonin-receptor-like receptor and the receptor-activity modifying proteins to dictate the ligand specificity of the receptor [14].Another possibility is that the lipid-facing residuesmay ... cross-linking for C5aR and AT1R-YFP. Because the rate ofcross-linking depends on the proximity of the sidegroups, the flexibility of the structures containing the cysteine probes, and the oxidizing...
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Báo cáo khoa học: Detergent-resistant membrane fractions contribute to the total 1 H NMR-visible lipid signal in cell potx
... however, neither the choline headgroups of PtdCho and sphingomyelin (exceptfor a small peak in THP-1) nor the sphingosine chain ofsphingomyelin were visible in the NMR spectra of DRMs. In bilayer ... DRMsare rich in cholesterol and sphingolipids (including GM1 and GM3) and selectively retain a number of proteinsincluding CD4, GPI-anchored proteins such as CD48[11,12], and proteins associated ... s. Sine–bell window functions were applied in the t1dimension, and Gaussian–Lorentzian window functions were applied in the t2dimension according to [7]. Zero filling was used to expand the...
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Báo cáo khoa học: Irreversible cross-linking of heme to the distal tryptophan of stromal ascorbate peroxidase in response to rapid inactivation by H2O2 ppt
... cross-linked to the APX apoprotein.Identification of the heme-binding amino-acidresidue in H2O2-inactivated stromal APX To determine which amino-acid residue in the apopro-tein was cross-linked ... of the apoprotein of the catalytic site(Fig. 4B). Because the indole ring of Trp35 is 3.18 A˚from the porphyrin ring in tsAPX (the distancebetween nitrogen of the indole ring and C6 of the ... to atryptophan residue facing the distal cavity. Mutation of this tryptophan to phenylalanine abolished the cross-linking and increased the half-time forinactivation from < 10 to 62 s. In...
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