Báo cáo khoa học: "Failure to Use Cubicles and Concentrate Dispenser by Heifers after Transfer from Rearing Accommodation to Milking Herd" pot

... 42 no. 1, 2001Failure to Use Cubicles and Concentrate Dispenser by Heifers after Transfer from Rearing Accommodation to Milking Herd By H. P. Kjæstad1 and H. J. Myren21The Norwegian School ... factors found to be signiﬁcant for178 H. P. Kjæstad & H. J. MyrenActa vet. scand. vol. 42 no. 1, 2001Kjæstad HP, Myren HJ: Failure to use cubicles and concentrate dispenser by heifers after ... been in use for at least one year and with a herd size of less than 60 cows, were contacted and asked to participate in a study.The study focused on heifers use of cubicles and concentrate dispenser...

báo cáo khoa học: "Failure of functional imaging with gallium-68DOTA-D-Phe1-Tyr3-octreotide positron emission tomography to localize the site of ectopic adrenocorticotropic hormone secretion: a case report" ppsx

... imagingmodalities for localizing the anatomical site of ectopicadrenocorticotropic hormone (ACTH) secretion are lim-ited. Somatostatin receptor scintigraphy (SRS), using theligand 111-indium-pentetreotide, ... technique used, but it’ suseful-ness has been questioned [1] . Recently, positro n emis-sion tomography (PET) scanning using gallium-68-DOTA-D-Phe1-Tyr3-octreotide (DOTATOC) has beenreported to ... lymph node(Figure 1).Due to the wide range in sensitivity and specificity ofthehighdosedexamethasonesuppression test (59-92% and 67-100%, respectively) and the inability to success-fully catheterize...

Báo cáo khoa học: Inducible knockout mutagenesis reveals compensatory mechanisms elicited by constitutive BK channel deﬁciency in overactive murine bladder pot

... Sweden). To minimizedifferences in protein expression caused by unequal afﬁnityof CyDyes, Cy3 and Cy5 were swapped between WT and BK) ⁄ )samples. Labeled WT and BK) ⁄ )samples and internal standard ... mmdipotassium-ATP and 10 mm Hepes (pH 7.4), and the freeCa2+concentration was adjusted to 300 nm. The holdingpotential was )10 mV, and test pulses of 300 ms durationwere applied every 5 s to ... sutures.Inhalation anesthesia was stopped, and mice were trans-ferred to their home cages and kept under infrared light for2 h. Thereafter, the mice were allowed to recover from thesurgical procedure...

Báo cáo khoa học: Enhancement of intracellular concentration and biological activity of PNA after conjugation with a cell-penetrating synthetic model peptide docx

... measured by CLSM according to [19] incytosol and nucleus of CHO cells and cardiomyocytes. Exposure to 0.5 lMI or 0.2 lMII for 30 min at 37 °C and subsequent re -exchange(RE) into empty ... Coulter, Fullerton, CA, USA), and were no rmalized to the area of the internal standarde-ﬂuoresceinyl lysine in order to eliminate irregularities ofinjection and buffer status. Because the exact ... for the Care and Use ofLaboratory Animals (NIH) and were approved by the localgovernment.The chronotropic response of the CM was measured asdescribed p reviously [16] on day 4 after seeding,...

Báo cáo khoa học: "A Method for Effective and Scalable Mining of Named Entity Transliterations from Large Comparable Corpora" doc

... positives but also to be computationally efficient. Thirdly, the use of linguistic tools and resources must be kept to a minimum as resources are available only in a handful of languages. ... perhaps due to the differences in the data used for training the NER and the data on which we used it. 4.4 Performance Measures Our intention is to measure the effectiveness of MINT by comparing ... Here, jt (and resp. is) denotes the jth (and resp. ith) character in wT (and resp. wS) and maA1is the hidden alignment between wT and wS where jtis aligned to jas,...

Báo cáo khoa học: Crystal structures of open and closed forms of D-serine deaminase from Salmonella typhimurium – implications on substrate speciﬁcity and catalysis pptx

... proton points towards Lys116 and Asp236 and not towards Thr166 or Tyr214.Therefore, Lys166 or Asp236 may be involved in deg-radation of l-Ser by St DSD.Based on ﬂuorescence energy transfer and ... catalysisDegradation of d-Ser to pyruvate and ammonia by StDSD involves two steps. In the ﬁrst step, d-Ser isconverted to aminoacrylate by Ca proton abstraction and protonation of the hydroxyl group ... racemase from Rattus norvegicus and Homo sapiens [30], and OASS from S. typhimurium[31]. In tryptophan synthase b from E. coli [32] and l-serine dehydratase from Rattus norvegicus (rat liverLSD)...

Báo cáo khoa học: Site-directed mutagenesis, kinetic and inhibition studies of aspartate ammonia lyase from Bacillus sp. YM55-1 pptx

... 3-nitropro-prionate as a potent competitive inhibitor and potentialcrystallographic ligand for AspB could help to identifythe additional features that are necessary for a fullyactive and speciﬁc aspartase.V. ... portion was transferred to a 10 mmquartz cuvette and the enzyme activity was assayed by theaddition of a small quantity (1–10 lL) of sodium l-aspar-tate (1) from a stock solution. The stock solutions ... ofa bound ligand) was recently solved (Fig. 1) and showsthat the overall topology and active site architecture ofAspB are similar to those observed in AspA and fuma-rase C (FumC) from E. coli,...

Báo cáo khoa học: Modulation of P-glycoprotein-mediated multidrug resistance by acceleration of passive drug permeation across the plasma membrane potx

... cellsTMRM efflux was monitored in cells whose mitochondrialmembrane potential was dissipated by CCCP. Under theseconditions, the dye efflux was rapid and was monitored by flow cytometry. K562 cells ... uptake and efflux of daunorubicin into and out of drug-resistant and drug-sensitivecells. The effects of the above-mentionedagents on daunorubicin uptake and effluxinto and out of drug-resistant and ... transfer into thenucleus, and binding to the DNA. To assess the effectof modulators specifically on drug transport across thecell plasma membrane, we characterized the effect ofthe modulators...

Báo cáo khoa học: Interaction with model membranes and pore formation by human steﬁn B – studying the native and preﬁbrillar states docx

... amyloidoligomers concentrate and undergo conformationalchange. The process is influenced by direct binding to different gangliosides and by cholesterol content[20,21].Stefin B belongs to the family ... excess of calcein was removed from the calcein-loaded liposomes by gel filtration on a small G-50column. Vesicles were stored at 4 °C immediately after preparation and used within 2 days. For calcein ... super-natant was transferred to another tube and the releasedcalcein measured using a Jasco FP-750 spectrofluorimeter(Jasco Inc., Easton, MD, USA), with excitation and emission at 485 and 520 nm....

Báo cáo khoa học nông nghiệp " FRUIT FLIES AND THEIR CONTROL BY USING PROTEIN BAIT IN VIETNAM " pdf

... distribution and species and levels of infestation by fruit fly and the use of protein baits to control fruit flies. These papers display the results at some locations in 2001 to 2007. 2. Materials and ... commercial/edible and wild fruits and incubating them in container for two to three weeks in laboratory to determine where flies emerge from the fruits Levels of infestations Collecting samples to find ... September and early October. Chemical control fruit fly was used but had a given low effect, and it has disadvantages of being unsafe for the environmental and health of people. Protein bait was used...

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