Báo cáo y học: " Immature cell populations and an erythropoiesis gene-expression signature in systemic juvenile idiopathic arthritis: implications for pathogenesis" ppt
Báo cáo y học: " Immature cell populations and an erythropoiesis gene-expression signature in systemic juvenile idiopathic arthritis: implications for pathogenesis" ppt
... sets, many representingfetal and embryonic hemoglobins (h emoglobins δ, μ, g, and θ), erythrocyte structural proteins (erythrocytemembrane protein band 4.2), surface proteins (glyco-phorins A, ... Further-more, an expansion of precursor cells and upregulationof an erythropoiesis gene-expression signature occurredpredominantly in individuals with sJIA and anemia.Strikingly, a discrepancy was noted ... and anti-CD19-APC; (f) anti-CD34-FITC, anti-CD105-PE, anti-CD45-PerCP, and anti-CD33-APC; (g) anti-Lin-FITC,anti-HLA-DR-PE, and anti-CD11c-APC; and (h) anti-Lin-FITC, anti-BDCA4-PE, and anti-CD11c-APC....
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Báo cáo y học: "T-cell senescence and contraction of T-cell repertoire diversity – catalysts of autoimmunity and chronic inflammation" pdf
... multiplesclerosis, and ankylosing spondylitis [60–64]. In general,these cells were clonally expanded and included autoreac-tive T cells, implicating them directly in the pathogenesisof these diseases. In ... Fulbright for assistance in manuscript prepara-tion and for preparing the graphics and Linda H Arneson for secretarialsupport.Arthritis Research & Therapy Vol 5 No 5 Goronzy and WeyandFigure ... goronzy.jorg@mayo.eduArthritis Research & Therapy Vol 5 No 5 Goronzy and Weyand231with ankylosing spondylitis. Namekawa and colleagues[54] demonstrated the presence of these cells in the syn-ovial...
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Báo cáo y học: " High cell density and latent membrane protein 1 expression induce cleavage of the mixed lineage leukemia gene at 11q23 in nasopharyngeal carcinoma cell line" ppt
... onfirmed by Westernblotting using anti-V5 (Fig. 4A) and S12 anti-LMP1antibody (Fig. 4B). Expression was demonstrated in LMP1 transfectants (Fig. 4A, lanes 2 and 4; Fig 4B, lanes3 and 5) as ... polymerase (Finnzyme, Finland). PCR cyclecondition was: 1 cycle at 98°C for 30 sec, followed by 30cycles at 98°C f or 10 sec, 61°C for 30 sec, 72°C for 15sec and a final cycle at 72°C for ... × 105(lanes 1 and 4), 2 × 105(lanes 2 and 5) and 4 × 105(lanes 3 and 6) were harvested for genomic DNA extraction after4 days of growth. DNA was digested with BamH I and analyzed on1%...
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Báo cáo y học: "Heterogeneity of CD4+ and CD8+ memory T cells in localized and generalized Wegener’s granulomatosis." potx
... Datawere collected for 104lymphocytes. Positively and nega-tively stained populations were calculated by quadrant dotplot analysis determined by isotype controls.Statistical analysisA non-normal ... ligand for the CC-chemokine receptors CCR1, CCR3 and CCR5, hasrecently been demonstrated in pulmonary granulomatouslesions in WG. The chemokine is expressed mainly in macrophages and may promote ... step, stain-ing for CD4, CD45RO and CCR5 was performed. At thehighest RANTES concentration, CCR5 staining wasalmost completely blocked. Cell- surface marker staining and flow cytometryPBMC were...
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Báo cáo y học: "Gene expression profile and synovial microcirculation at early stages of collagen-induced arthritis" pot
... described by Nanakumar and coworkers [12], scoring ofanimals was done blindly using a scoring system based on thenumber of inflamed joints in each paw, inflammation beingdefined by swelling and redness. ... Germany) and transferred to a S-VHS video system for subsequent offline analysis.Microcirculatory analysis For quantitative offline analysis a computer-assisted microcir-culation image analysis ... receptors for β1/β2 integrins, P-selectin is the predom-inant adhesion molecule, mediating monocyte binding toinflamed synovial venules [23]. Similarly, increased cellularinfiltration and increased...
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Báo cáo y học: "Analysis of C4 and the C4 binding protein in the MRL/lpr mouse" pptx
... experiments and was primary author of the manuscript. KS performed thehemolytic assays and assisted in interpreting the data.RAW generated the knockout mice, assisted in interpretingthe data, and ... 1983, 209:847-856.9. Lappin DF, Whaley K: Interferon-induced transcriptional and post-transcriptional modulation of factor H and C4 binding-protein synthesis in human monocytes. Biochem J 1990,271:767-772.10. ... withperivascular monocytic infiltrates, and increased C3 and IgMstaining. The patient was diagnosed with atypical Behcet'sdisease and was treated with solumedrol and cyclophospha-mide. Genotyping was...
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Báo cáo y học: "Caveolin-1 expression and stress-induced premature senescence in human intervertebral disc degeneration" docx
... N(epsilon)-(carboxymethyl)lysine in human tissues in diabetes and aging. J Clin Invest 1997,99:457-468.37. Le Maitre CL, Freemont AJ, Hoyland JA: The role of interleukin-1 in the pathogenesis of human intervertebral ... Hoyland JA, Freemont AJ: Interleukin-1 receptorantagonist delivered directly and by gene therapy inhibitsmatrix degradation in the intact degenerate human interverte-bral disc: an in situ zymographic ... (incorporating annulus fibrosus [AF] and NP in continuity) was fixed in 10% vol/vol neutral buffered formalin and embedded in paraffin wax. Four micron sections werestained with haematoxylin and eosin to...
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Báo cáo y học: " hzAnalyzer: detection, quantification, and visualization of contiguous homozygosity in high-density genotyping datasets" doc
... variants.Materials and methodshzAnalyzerhzAnalyzer is an R [19] package t hat uses Java classes for detecting runs of contiguous homozygous genot ypes and R functions for quantifying the frequency and extent ... extent and frequency ofhomozygous segments with haplotypes underlying extAUCpeaks. Analysisof the consistency of the homozygous extent distribution and length and frequency of haplotypes for ... methods for extracting homozygous segments from high-densitygenotyping datasets, quantifying their local genomic structure , identifying outstanding regions within the genome and visualizing results...
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Báo cáo y học: "Treatment of hypertriglyceridemia and HIV: fenofibrate-induced changes in the expression of chemokine genes in circulating leukocytes" ppsx
... final version of the manuscript. GA, RB-D and AR helped in the data analysis and interpretation. JJ and JC supervised the laboratory determinations. Allauthors have read and approved the final ... presumably increasing SDF-1 values.SDF-1 plays a significant role in HIV infection because itis the natural ligand for CXCR4 which is used by HIV T-tropic strains to enter into the cells in advanced ... Stanley T, Wilson PW, D'Agostino RB, Grinspoon S:Metabolic abnormalities and cardiovascular disease risk fac-tors in adults with human immunodeficiency virus infection and lipodystrophy....
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Báo cáo y học: "T cell Activation does not drive CD4 decline in longitudinally followed HIV-infected Elite Controllers" docx
... the study, designed and optimized the antibody panel,performed the experiments, analyzed the data and prepared the manuscript.CMT and SB aided in study design, and critical review of manuscript. ... anti-CD38, allophycocyanin (AP C) conjugated anti-HLA-DR, and peridinin chlorophyll protein (PerCP) anti-CD3 anti-bodies (BD Biosciences, Mississauga, Canada) for 30 min-utes in the dark. In parallel, ... stainedwith PE- and APC-conjugated immunoglobulin isotypecontrol antibodies (BD Biosciences) and used to set gates for defining positive staining. Analysis was performed ona FACSCalibur instrument...
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