... was greater than 30 lM.Inthis case the exact Kdvalue was not assessed by Scatchardanalysis because the highest SOCS-3 concentration was30 lMand a calculation by the BIAEVALUATIONsoftwareFig. ... Matsumoto, A. , Masuhara, M., Mitsui, K., Yokouchi, M.,Ohtsubo, M., Misawa, H., Miyajima, A. & Yoshimura, A. (1997)CIS, a cytokine inducible SH1 protein, is a target of the JAK-STAT5 pathway ... subtracted nonspecificbinding. Determination of the dissociation constant wascarried out by Scatchard analysis [22].Peptide precipitation assay and immunoblot analysisApproximately 0.15 lmol of...