... 1-2% of asthmatic and 7-9% of cysticfibrosis (CF) patients. Acute flares of ABPA are charac-terized by wheezing, pulmonary infiltrates, eosinophilia,increased levels of total IgE, and increased ... 0.04 and p, 0.04, respectively).Cytokine synthesis In Alternaria-sensitive moderate-severe asthma, Alter-naria extract stimulated lymphocytes had significantlyincreased synthesis of IL-5 and ... supernatant cytokines and chemokines was per-formed by Flex Cytometric Bead Assay (BD Pharmin-gen) to measure IL-4, IL-5, IL-10, IL-13, IFN-g,synthesis, as previously described [27,28].HLA typingIn...