báo cáo khoa học: " Insight into the early steps of root hair formation revealed by the procuste1 cellulose synthase mutant of Arabidopsis thaliana" doc
báo cáo khoa học: " Insight into the early steps of root hair formation revealed by the procuste1 cellulose synthase mutant of Arabidopsis thaliana" doc
... Plant BiologyOpen AccessResearch article Insight into the early steps of root hair formation revealed by the procuste1 cellulose synthase mutant of Arabidopsis thalianaSunil K Singh1,4, Urs ... BLocalisation of root hair formation is altered in the prc1-1 mutantFigure 3Localisation of root hair formation is altered in the prc1-1 mutant. The positions of root hairs relative to the basal ... use of the procuste1 (prc1-1) cellulose deficient mutant of Arabidopsis to probe the influence of cell wall structure in modulating root hair formation. The Arabidopsis prc1-1 is mutated in the...
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Báo cáo khóa học: Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy pptx
... direct exploitation of the structural results of the former to explain the mechanism of CaM activation of the latter. Crystallizationand resolution of the 3D structures of AC and AC–CaM,which ... and therefore provides informationmainly on the dynamics of this region of the protein. Thisresidue is probably widely accessible to the solvent in AC asshown by the maximum wavelength of the ... respectively, the values of the center and the normalized amplitude of each lifetime peak.cIiare the values of the partialintensity of the ithcomponent Ii¼ aisi/<s>.d The mean...
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Báo cáo khoa học: Insight into the phosphodiesterase mechanism from combined QM ⁄ MM free energy simulations pot
... with other residues(Fig. 1). The hydrogen atoms of H2O2 form hydrogenbonds with the side chain of Glu304 and the backbone of Thr345. Interestingly, the side chain of Thr345,together with the ... to model the hydrolysis of cAMP by the enzyme PDE4B, which provides furtherinsights on the general features of phosphate hydrolysis. The PDE superfamily of enzymes can be classified into 11 members ... Although the structure of a small frag-ment of PDE4D was reported in 1996 [5,13], keyinsights into the understanding of the catalytic activesite of PDEs were obtained following the determinationof...
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Báo cáo khoa học: Proteoglycans in health and disease: novel regulatory signaling mechanisms evoked by the small leucine-rich proteoglycans ppt
... in softagar by increasing the expression of the cyclin-depen-dent kinase inhibitor p21WAF1[72]. Again, similar todecorin, these growth inhibitory effects of lumicanoccur in a variety of ... prostate carcinoma generated by a targeted deletion of the tumor suppressor PTENin the prostate, systemic delivery of decorin causes amarked downregulation of the EGFR in the treatedtumors with ... migration by affecting the IGF-IR pathway[61]. All of these studies were performed with ‘nor-mal’ cells. Thus, there are no published data on the role of decorin in modulating cancer growth via the IGF-IR...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
... [20,21].In the present study, the first insights into the secon-dary and tertiary structure of PLD a2 from cabbageare given. The surface structure of the enzyme wasinvestigated by small-angle ... confirmed by the calculation of the a-helix and b-strand contents of the protein with the online programs dichroweb[25] and k2d [26], respectively. The well-structured near-UV CD spectrum of PLDa2(Fig. ... of the enzyme.These results unambiguously show that activation of PLDa2 by calcium ions is due to the binding of cal-cium ions to the enzyme, and not (or not only) to bet-ter structuring of...
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Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc
... iscomposed of residues 25–540. The overall structure of the model of IPU and the mutated residues in this study areshown in Fig. 3. To elucidate the mechanism of the substrate recognition of IPU, ... activating the nucleophilic water m olecule) in the first step of the reaction.A carbonium ion intermediate subsequently forms, and isfurther attacked by the water molecule. The study of site-directed ... the origin al StyI–XbaI fragment of pIPA118(–) was replaced with the mutated StyI–XbaIfragment. The EcoRI–BamHI fragments o f these plasmidswere further subcloned into pHIL-S1.Expression of...
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Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate specificity by replacement of Asn149 with Asp docx
... as a consequence of the mutagenic replacements.Effects of the His120Asn and His145Asnmutations on the affinity of metal bindingto arginase IITo further examine the effects of the His120fiAsnand ... produced by agmatine hydrolysis, in studying the inhibitory effect of agmatine on arginine hydrolysis, reactions were followed by measuring the formation of ornithine, determined by the method of Chinard ... addition to removal of the more weakly bound Mn2+, the lower affinity for thatmore tightly bound to the protein may explain the absence of Mn2+from the EDTA-treated species of the His145Asn variant....
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Báo cáo khoa học: Insights into substrate and product traffic in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt
... analysis of initial rate data in the absence of inhibitors, we used the specific equation in Scheme 1. The effect of two ligands on the activity of the wild-type DmAChE was evaluated by the equation ... substrateentrance. Thus, in the wild-type, the channel is so narrow that the ‘backdoor’ is used by at most 5% of the traffic, with the majority of traffic pass-ing through the main entrance. In mutants Trp83Ala ... by the crystal structures(protein databank codes 1DX4 and 1QO9). One of these conformations results in the formation of a chan-nel approximately 5 A˚in diameter, connecting the gorge to the...
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Báo cáo khoa học: Investigations into the ability of an oblique a-helical template to provide the basis for design of an antimicrobial anionic amphiphilic peptide pot
... hypo-thesis of Tytler et al. [48], it may be that insertion of the inverted wedge shape formed by the AP1 a-helix into membranes of E. coli leads to the formation of nonbilayer structures and thereby ... pressure of the monolayer, and Arepresents the area per molecule in the monolayer. The Gibbs free energy of mixing (DGMix) quantifies the sta-bility of monolayer mixtures, thereby providing informationon ... dynamicbehaviour of monolayers. The compressibility modulus,CÀ1s, provides a measure of the compressional elasticity of a monolayer and can be used to characterize the phase state of the isotherm, thereby...
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Báo cáo khoa học: Insights into the activation of brain serine racemase by the multi-PDZ domain glutamate receptor interacting protein, divalent cations and ATP ppt
... together withPDZ7. Interestingly, in the absence of additional ATP, the activation induced by the presence of the PDZ4-PDZ5-PDZ6-GAP2-PDZ7-end of GRIP reached the highest value. The conformational ... carbonylgroups. Both the geometry and the distances perfectlyagree with the coordination of these metals [23]. The Fig. 4. Binding of ATP to SR changes the Vmax of the racemization reaction but not the oligomeric ... that these enzymes establish identicalmonomer–monomer interactions in the presence andabsence of the nucleotide. According to our results, the absence of modulation of the oligomeric state of the...
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Từ khóa: Nghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namđề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giảiBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitQuản lý hoạt động học tập của học sinh theo hướng phát triển kỹ năng học tập hợp tác tại các trường phổ thông dân tộc bán trú huyện ba chẽ, tỉnh quảng ninhNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Thơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt namMÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲQUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ