... helicase and E. coli helicase II were not inhibited by actinomycin C 1[22]. Actinomycin C 1, a polypeptide containing the properties of an antibiotic, intercalates into dsDNA and thereby inhibits ... lLofthesolvent(dimethyl sulfoxide), and lanes 3–10 are reactions in the presence of camptothecin, ethidium bromide, daunorubicin, nogalamycin, actinomycin C 1, VP-16, novobiocin and m-AMSA. The ... doi:10.1046/j.1432-1033.2003.03532.x In order to understand the mechanism of DNAunwinding we tested the effect of different compounds and found that actinomycin C 1, ethidium bromide,daunorubicin and nogalamycin inhibited the...