Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E coli" ppt
Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E. coli" ppt
... The fact that we wereable to < /b> detect the same band by three different antibodiestargeting different components of < /b> the chimeric RTB /p24 < /b> protein,< /b> demonstrate the integrity of < /b> the protein < /b> after purification < /b> ... the expression in E. coli of < /b> HIV-1 < /b> p24 < /b> fused < /b> to < /b> the subunit B of < /b> ricin < /b> toxin.< /b> The high levels of < /b> antibodies obtained after intranasal and < /b> intraperitonealimmunization of < /b> mice demonstrate the ... JournalOpen AccessResearchProduction and < /b> purification < /b> of < /b> immunologically < /b> active < /b> core < /b> protein < /b> p24 < /b> from < /b> HIV-1 < /b> fused < /b> to < /b> ricin < /b> toxin < /b> B subunit in E. coliAlberto J Donayre-Torres1, Ernesto Esquivel-Soto2,...
- 11
- 292
- 0
Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx
... shown in E. coli that theseenzymes are responsible for the formation of < /b> threon-ine from < /b> glycine in vitro and < /b> in vivo [29]. However,the primary role of < /b> this pathway is believed to < /b> bethreonine ... s)1Æmm)1).DiscussionThree pathways for threonine degradation are known.Threonine aldolase (EC 4.1.2.5) is responsible for theconversion of < /b> threonine into acetaldehyde and < /b> glycine.The threonine dehydratase (EC ... study. With respect to < /b> the otherputative alcohol dehydrogenases, a more elaboratescreening is currently being performed to < /b> obtaininsight into their substrate specificity and < /b> possiblytheir physiological...
- 8
- 415
- 0
Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... acids of < /b> the active < /b> site are well conservedbetween the proteins, whereas the C-terminal domainsare less conserved.Overexpression of < /b> the tyrosinase gene in Trichoderma reeseiTyrosinase production ... corresponding cDNA were clonedby PCR and < /b> sequenced in order to < /b> verify the sequenceat the genome website, to < /b> exclude PCR mutations and< /b> to < /b> localize the introns. The gene is interrupted by sevenshort ... reesei TYR2 in the dipeptide glycine–tyrosine and < /b> the tripeptideglycine–glycine–tyrosine. The relative oxidation rateincreased as the length of < /b> the peptide increased. Simi-larly, protein-< /b> bound...
- 14
- 650
- 0
Báo cáo khoa học: Production and utilization of hydrogen peroxide associated with melanogenesis and tyrosinase-mediated oxidations of DOPA and dopamine docx
... 2005 FEBS 2415ize to < /b> form pigment. The identity and < /b> mode of < /b> action of < /b> the enzymes involved in the different steps of < /b> mel-anogenesis have long been intensely investigated, in large measure to < /b> elucidate ... Compared to < /b> tyrosinase,disproportionately less effort has been given to < /b> under-standing the role of < /b> peroxidase in the early stages of< /b> melanogenesis, despite reports of < /b> the involvement of< /b> peroxidase–H2O2systems ... of< /b> peroxidase–H2O2systems in later stages during theoxidation of < /b> indolequinone precursors of < /b> eumelanin and < /b> benzothiazinylalanine precursors of < /b> pheomelanin[5–10]. Of < /b> considerable interest are recent...
- 9
- 393
- 0
Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt
... showed that the deleted NH2-terminal b- hairpin of < /b> ribotoxins is involved in theallergenic response to < /b> the wild-type allergens.Binding of < /b> human specific IgG present in the abovepool sera to < /b> ... any receptor interaction [1].Once inside the host cell, ribotoxins inhibit protein< /b> biosynthesis by inactivating the ribosomes leading to< /b> cell death [2]. They cleave a unique phosphodiesterbond ... amino acid sequence variability among ribotoxins [9].This NH2-terminal b- hairpin is a highly flexible and< /b> exposed region of < /b> these proteins, folded independently from < /b> the protein < /b> core < /b> [7,10]...
- 9
- 517
- 0
báo cáo khoa học: " Production and quality of clinical practice guidelines in Argentina (1994–2004): a cross-sectional study" pps
... inform recommendations, this type of < /b> evi-dence was rarely referred in Argentine guidelines [22].Therefore, networking activities betweeen guideline pro-ducers and < /b> Argentine Cochrane Centers shoud ... Argentine guidelinereported conflict of < /b> interests or funding sources. Lack of< /b> Table 4: Scores by item of < /b> the AGREE instrumentDomains and < /b> items of < /b> the AGREE instrument Median value Interquartile ... Explicit inclusion and < /b> exclusion criteria were used to < /b> select guidelines.Each CPG was independently assessed by two reviewers using the AGREE instrument. Domainscores were calculated as recommended...
- 10
- 226
- 0
Báo cáo y học: " Production and purification of VP2 protein of porcine parvovirus expressed in an insectbaculovirus cell system" docx
... White colo nies cont ainedthe recombinant bacmid, and < /b> therefore were selected forisolation of < /b> recombinant bacmid DNA. Before DNA wasisolated, candidate colonies were streaked to < /b> ensure theywere ... baculovirus. lane 1, the protein < /b> expressed in insect cells challenged with negative baculovirus; lanes 2 to < /b> 6, the protein < /b> expressed 1 to < /b> 5 days after insect cells were challengedwith recombinant baculovirus; ... VP2 protein < /b> in sf9 cellsThe recombinant baculoviruses, containing the codingsequences of < /b> VP2 with t he polyhistidine tag at the N-terminus, were generated by using the Bac -to-< /b> BacTMsystem (Invitrogen;...
- 6
- 387
- 0
Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf
... revealed unexpected protein< /b> protein < /b> interac-tions interfering with efficient mitochondrial protein< /b> turnover in the case of < /b> human cytosolic aconitase.These results emphasize specific features of < /b> ... transferred to < /b> apoly(vinylidene fluoride) membrane. The band corres-ponding to < /b> m-hIRP1 was cut and < /b> its N-terminalsequence was determined. From < /b> this single band, twoN-terminal sequences were obtained. ... of < /b> intense interest, particularly in S. cerevisiae. An extensive set of < /b> proteins participa-ting in different stages of < /b> the process has been discov-ered. More than 10 such proteins have been found...
- 10
- 422
- 0
Báo cáo khoa học: Structure and mechanism of the ThDP-dependent benzaldehyde lyase from Pseudomonas fluorescens potx
... thecontraction seems to < /b> be caused by an increase of < /b> thePEG 200 concentration from < /b> 50% to < /b> 55%, removingwater from < /b> the protein.< /b> Comparison with related proteins To < /b> find related proteins in the ... other proteins showed that benzaldehyde lyase belongs to< /b> a group of < /b> closely related ThDP-dependent enzymes. The ThDP cofactors of < /b> these enzymes are fixed at their two ends in separate domains, ... uses the same educts to < /b> produce the S-enantiomer [29]. In order to < /b> explorethe geometry of < /b> the reaction catalyzed by BAL, R-ben-zoin was modeled into its active < /b> centre (Fig. 7). Theresulting...
- 10
- 454
- 0
Báo cáo khoa học: Expression and characterization of recombinant 2¢,5¢-oligoadenylate synthetase from the marine sponge Geodia cydonium ppt
... recombinant protein < /b> preparation,where the number of < /b> nucleotides was decreased by nuclease treat-ment. The number of < /b> nucleotides per protein < /b> monomer was esti-mated as described in Experimental ... vertebrate lineages, the elucidation of < /b> thefunction of < /b> the 2-5A synthetase in these invertebrates,particularly in the innate immune system, would be of< /b> considerable interest.Before the present study ... shown).Figure 1 demonstrates the results of < /b> the purification of< /b> the recombinant proteins. The occurrence of < /b> dominantbands of < /b> the recombinant proteins provides evidence of < /b> a high degree of < /b> purification obtained...
- 13
- 429
- 0
Từ khóa: báo cáo khoa học mẫubáo cáo khoa học y họcbáo cáo khoa học sinh họcbáo cáo khoa học nông nghiệpbáo cáo khoa học lâm nghiệpbáo cáo khoa học thủy sảnbáo cáo khoa học về cá trabáo cáo khoa học nghiên cứu chôm chômtrạng thái hiện sinh báo cáo khoa họcbiểu tượng văn học báo cáo khoa họctài liệu báo cáo khoa họccách trình bày báo cáo khoa họcbáo cáo khoa học toán họccách làm báo cáo khoa họctrình bày báo cáo khoa họcBáo cáo quy trình mua hàng CT CP Công Nghệ NPVchuyên đề điện xoay chiều theo dạngNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Định tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtChiến lược marketing tại ngân hàng Agribank chi nhánh Sài Gòn từ 2013-2015MÔN TRUYỀN THÔNG MARKETING TÍCH HỢPTÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ