Báo cáo khoa học: " Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences" docx

... characteristics. AAV is capable of infecting dividing and non-dividing cells in vitro and invivo and of infecting cells originating from multiple spe-cies and tissue types. No human disease has ... not for citation purposes)Virology JournalOpen AccessResearch Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory ... that of wt AAV (Figure 5).DiscussionDifficulty in generating high titers of rAAV is an ongoinglimitation in the application of rAAV technology to genetherapy. In the present studies, we investigated...

Báo cáo khoa học: Characterization of recombinant prolidase from Lactococcus lactis – changes in substrate speciﬁcity by metal cations, and allosteric behavior of the peptidase pdf

... changes maytrigger the allosteric behavior of Lc. lactis prolidase.Another possibility is that Ser307 works as a key resi-due in the allosteric behaviour of this enzyme. Thisresidue is located ... puriﬁcation of recombinant Lc. lactis prolidaseThe overexpression of recombinant proteins can dis-turb host cell metabolism through their activities. Toavoid these negative effects, host cells often ... physiological activity. As a consequence, there is little information on the expression of recombinant prolidases, the functionality of each residue or proteinengineering of this enzyme.In the present...

báo cáo khoa học: " Production of viable male unreduced gametes in Brassica interspecific hybrids is genotype specific and stimulated by cold temperatures" potx

... Brassica relative Arabi-dopsis result in high frequencies of dyads and triadsthrough the same mechanism of parallel spindles atmeiosis II (Additional file 1) [5,27,32]. A single gene is thought ... recombination: introgression of resistance to podshatter in Brassica napus. Genetics Research 1990, 56:1-2.14. Morinaga T: Interspecific hybridisation in Brassica II. The cytology of F1hybrids of ... permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.grains) was 1.22%, which was similar to estimates of unreduced nuclei at the sporad...

Tài liệu Báo cáo khoa học: Transcription of individual tRNA1Gly genes from within a multigene family is regulated by transcription factor TFIIIB pdf

... element in tRNAGly1-6,7 wassigniﬁcantly distant from the TFIIIC binding region.In tRNAGly1-6,7 binding of TFIIIB, even in the pres-ence of TFIIIC, was dissociated by heparin, unlikeTFIIIB ... transcription site is achieved by interactions between the associated factors [16,17].TFIIIB is analogous to the pol II-speciﬁc factor, KeywordsBombyx mori; differential transcription;RNA pol III; ... theweakly interacting TFIIIB complexes, the amounts of TFIIIB–promoter complexes that remain after heparinstripping provide a measure of its stable interaction [12,13]. Formation of TFIIIC ⁄ TFIIIB...

Báo cáo khoa học: Expression of the Drosophila melanogaster ATP synthase a subunit gene is regulated by a transcriptional element containing GAF and Adf-1 binding sites pptx

... the expression of the Drosophila a-F1-ATPasegene. Indeed, this is highlighted by the presence of similarcombinations of the two tr anscription factors in thepromoter proximal region of the orthologous ... Drosophila aF1-ATPasegene. Initially, we mapped the transcription initiation sites by primer extension, S1 mapping analysis and mRNA 5¢-end a mpliﬁcation. In this way, we localized a small regioncontaining ... [28].Mapping of transcriptional initiation sitesIdentiﬁcation o f the a-F1-ATPase transcription start sitewas achieved by three different method s: primer extension,high-resolution S1 mapping and...

báo cáo khoa học: " Effects of enamel matrix derivative and transforming growth factor-b1 on human osteoblastic cells" pot

... activities, which include osteoid matrix production and mineralization. McCauley & Somerman[49] demonstrated that TGF-b1 inhibits the formation of mineralized nodules in vitro. In addition, ... matrix derivativestimulates proliferation but not differentiation of pre-osteoblastic 2T9cells, inhibits proliferation and stimulates differentiation of osteoblast-like MG63 cells, and increases ... raising the hypothesis that the action of EMDwould be mediated by growth factors found in its com-position or in the culture medium modif ied by cellsunder EMD exposure[15].The interactions...

Tài liệu Báo cáo khoa học: Production of biologically active forms of recombinant hepcidin, the iron-regulatory hormone docx

... structure is otherwise permissive for changes [17].Once in the circulation, the peptide acts to limitgastrointestinal iron absorption and serum iron levels by inhibiting dietary intestinal iron ... of body iron metabolism. Hepcidin is synthesized underthe form of a precursor, prohepcidin, which is processed to produce thebiologically active mature 25 amino acid peptide. This peptide is ... homeostasis. Hepcidingene expression is positively regulated by iron andinﬂammation and negatively regulated by anemia andhypoxia [5,12]. There is compelling evidence thatdysregulation of hepcidin...

Báo cáo khoa học: Production of a recombinant mouse monoclonal antibody in transgenic silkworm cocoons pptx

... retained within the cells.This inhibition of secretion is caused by the stableassociation of an endoplasmic reticulum-resident stressprotein, BiP, with the H-chain dimer [34–36]. Thismechanism ... CH3domain interface region distal to theglycosylation site in the CH2domain of IgG [29], andthe afﬁnity of the IgG-binding proteins for IgG is unchanged by deglycosylation of IgG [30]. Thus, it ... chaperone proteins involved in thesecretion of silk proteins might also function in that of recombinant IgG. Although the function of thesefactors in IgG-secretion is not sufﬁcient in MSG cells,as...

Báo cáo khoa học: Inhibition of recombinant human maltase glucoamylase by salacinol and derivatives pdf

... studies.Kinetic analysis conﬁrmed the enzyme activity of the recombinant protein, and inhibition analysis conﬁrmedclassic competitive inhibition by a-glucosidase inhibi-tors. Salacinol with a K i of ... of the lines corresponding to inhib-itor concentration approximately intersected at a pointat the y-axis indicating classic competitive inhibition.The experimentally determined inhibition constants ... synthesizedputative inhibitors (Fig. 1). Initially, each inhibitor wastested at a concentration of 200 lm in order to screenfor the most effective inhibitors. The inhibition results of the initial screening...

Báo cáo khoa học: Characterization of recombinant forms of the yeast Gas1 protein and identiﬁcation of residues essential for glucanosyltransferase activity and folding pot

... Sanita`Pubblica Veterinaria,Universita`degli Studi di Milano, Milano, Italy;5Dipartimento di Biotecnologie e Bioscienze, Universita`degli Studidi Milano-Bicocca, Milano, ItalyGas1p i s a glycosylphosphatidylinositol-anchored ... pathway.Given this prominent role of C74 in directing the folding of Gas1p, it is premature to assess whether it is involved inthe formation of a structurally important disulphide bond,and more direct ... host cells and in virulence. Thebiogenesis of the extracellular matrix is a fascinating aspect of yeast m orphogenesis. The elucidation of t he enzymaticactivities involved in its assembly could...

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