Báo cáo khoa học: "Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes" ppt

... 7:25http://www.virologyj.com/content/7/1/25Page 4 of 7RESEARC H Open AccessGenomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoesHuimin ... 129:283-290.doi:10.1186/1743-422X-7-25Cite this article as: Xu et al.: Genomic variability in Potato virus M and the development of RT-PCR and RFLP procedures for the detection of this virus in seed potatoes. Virology ... RT-PCR procedures were developed for the specific detection of PVM in various potato samples and for the confirmation of PCR amplicons. The efficacy of RT-PCR for indexing seed potato samples...

Báo cáo khoa học: Neuroserpin Portland (Ser52Arg) is trapped as an inactive intermediate that rapidly forms polymers Implications for the epilepsy seen in the dementia FENIB ppt

... is a member of the serineproteinase inhibitor or serpin s uperfamily. It inhibits the enzyme tissue plasminogen activator (tPA) and maybe important in regulating neuronal plasticity and memory[4–7]. ... and a-helical structure content as determined by the largeincrease in magnitude of the signal at 216 nm and the small increase at 222 nm. This proﬁle is comparable tothat obtained for monomeric ... loop of one molecule intob-sheet A of another [1,6]. The resulting s pecies is inactive asa proteinase inhibitor and accumulates in the endoplasmicreticulum in cell models of disease [8] and in...

Báo cáo khoa học: Genomic structure and expression analysis of the RNase j family ortholog gene in the insect Ceratitis capitata pptx

... the boxes indicate the size of the exons, and those below the lines indicate the size of the introns. (B) Multiple amino acid sequence alignment of the RNase j family orthologs. Amino acid sequencesare ... were alsofound in the 3¢ UTR, one of them just 46 bp upstream of the poly(A) tail. The remaining two poly(A) signalsare upstream of the region that contains the AUUUAmotifs and the poly(U) tracts, ... [28]. The complexity of ARE-mediated pathways is manifested by the presence of multiple ARE-binding proteins, including stabilizingproteins [29,30] or proteins that promote degradation of mRNAs...

Báo cáo khoa học: "Genetic variability of the prion protein gene (PRNP) in wild ruminants from Italy and Scotland" ppsx

... polymorphisms and comparing them with those in North American cervids and domestic small ruminants may provide an estimate of the susceptibility of these species to CWD and other TSEs.Materials ... A total of 323 red deer samples were collected from Scotland (n = 132; 82 from the mainland and 50 from the Isle of Rhum) and during the hunting seasons from Italy (n = 191). The chamois (n ... (Fig. 1), 79 and 136. The red deer population from the Isle of Rhum was in HWE for all the polymorphisms, while the populations from mainland Scotland and from Italy were not in HWE for SNPs at...

Báo cáo khoa học: "Morphological variability of oaks (Quercus robur L, Quercus petraea (Matt) Liebl, Quercus pubescens Willd) in northeastern France: preliminary results" pptx

... south, and always in the middle part of the first flush shoot. Ten of these 20 sampleswere chosen at random for measurements, after the elimination of broken, incomplete ... highlydiscriminating ones, and sometimes notdiscriminant at all. The best features, in our sample, are intercalary veins and pilos-ity development. Further studies in progress ... nint. number of intercalary veins(mean of 10 leaves per tree); lpet: length of petiole in mm (mean of 10 leaves pertree); I species index; positive for Q roburand...

Báo cáo khoa học: "Morphological variability of oak stands (Quercus petraea and Quercus robur) in northern Germany" docx

... the development of the auricle the number of inter-calary veins and the length of the petiole. Anequation was created to calculate the discrimi-nant score for single ... measurements are explained in figure 1.By means of the computer program SPSS X and the discriminant analysis, Q robur leaveswere separated from those of Q petraea. In ... show the typical combinations of charac-ters of the other oak species; these trees mustbe eliminated before seed collection;C) the proportion of the other oak species isso...

Báo cáo khoa học: " Spatial variability of humus forms in some coastal forest ecosystems of British Columbia" pdf

... (1973) Information theory and an extension of maximum likelihood principle. In: Second Inter-national Symposium on Information Theory (BNPetrov, F Csáki, eds), Akadémia Kiadó, ... withkriging. The magnitude of this error will be a mea-sure of the validity of estimation. The goodness of estimation can be determined by comparing the difference between the ... the otherhumus forms on these sites. In the Dou-glas-fir site, the distribution of mineralizable-N was skewed to the right, manifesting the presence of Lignomors - the...

Báo cáo khoa học: "Genomic alterations of primary tumor and blood in invasive ductal carcinoma of breast" pdf

... regions of gain present in more than30% of the primary tumor samples and 70 regions of gainpresent in more than 30% of blood samples. The mostfrequently gained region was chromosome 8q24. This region ... 3). Gain on 8q24.3 and 20q13.33 was iden-tified in more than 50% of the samples. Gain on17q11.2 was seen in 19 of the primary tumors (63%) and in 17 of the blood samples (57%). The regionincluded ... 98-100°C for 5 min for denaturation and then cooledon ice for 5 min. The denatured DNA was differentiallylabeled with 3 μl of 1 mM Cy3 and Cy5 conjugateddCTP (Perkin-Elmer, USA) by random primer...

Báo cáo khoa học: "Genomic and oncoproteomic advances in detection and treatment of colorectal cancer" ppt

... high incidence of BRAF mutations and micro-satellite instability (MSI) in a group of tumours with highmethylation frequency [30].Most recently, oncostatin M (OSM), a member of the interleukin-6 ... expression of secretagogin in endothelial cells of blood vessels in some meningiomas, haemangiopericytomas and hae-mangioblastomas led to the theory that it is implicated in angiogenic activity in human ... analysis of less abundantproteins. As in MALDI-TOF, ionisation again occurs usinglaser emission, and the peptide ions thus formed thenguided into the MS analyser. The first SELDI-TOF study attempted...

báo cáo khoa học: "Genomic profiling of plastid DNA variation in the Mediterranean olive tree" pdf

... orNED), 0.2 μmol of the reverse primer, 0.05 μmol of the forward primer, and 0.5 U of Taq DNA polymerase(Promega). The reaction mixtures were incubated in aT1 thermocycler for 2 min at 95°C, followed ... DNA template, 1×reaction bu ffer, 2 mM MgCl2, 0.2 mM dNTPs, 0.2 μmol of each primer, and 0.75 U of Taq DNA polymerase(Promega,Madison,WI,USA).Thereactionmixtureswere incubated in a thermocycler ... parsimony-informative in the olive tree complex), phylogenetic relationships weredepicted from the complete cpDNA genomes usingboth maximum parsimony (MP) and maximum likeli-hood (ML) techniques (Figure 2). The...

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