Báo cáo khoa học: " Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection" pps

... 7:13http://www.virologyj.com/content/7/1/13Page 4 of 7SHORT REPOR T Open Access Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infectionPhui San ... 61(2):111-117.doi:10.1186/1743-422X-7-13Cite this article as: Ho et al.: Establishment of one-step SYBR green-based real time-PCR assay for rapid detection and quantification of chikungunya virus infection. Virology Journal 2010 ... semi-quantitative RT-PCR assay, immunofluorescence detection method and the one-step SYBR Green-based RT-PCR assay in the detection of CHIKV infection revealed much rapid and increase sensitiv-ity of the latter...

Báo cáo khoa học: Methylcitrate synthase from Aspergillus fumigatus Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia ppt

... propionate and may count for the increased sensi-tivity of A. fumigatus towards propionate. A determin-ation of the Km-values for the substrates acetate and propionate was performed to proof that ... modelusing larvae of Galleria mellonellaInsects are quite often used as a model to study attenu-ation of virulence of pathogenic microorganisms. Espe-cially strains of Candida albicans and Pseudomonasaeroginosa ... internaldegradation of amino acids and an accumulation of propionyl-CoA as shown for A. nidulans [8]. There-fore, some accumulation of propionyl-CoA may alsooccur on glucose medium in the mutant strain and affect...

Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps

... al. Detection limit and standard curve of the multiplex real- time PCR The detection limit and standard curve of the multiplex real- time PCR was determined using S. Typhimurium ATCC 14028 and S. Enteritidis ... multiplex real- time PCR assay showed 100% specificity for Salmonella spp. and S. Typhimurium and 99.1% for S. Enteritidis. The multiplex real- time PCR assay showed 100% sensitivity for Salmonella ... times, real- time PCR assays have been successfully applied in the detection of bacterial pathogens in food products [11,12,24,25]. A single real- time PCR assay was applied for specific detection...

Báo cáo khoa học: "Preoperative Y-90 microsphere selective internal radiation treatment for tumor downsizing and future liver remnant recruitment: a novel approach to improving the safety of major hepatic resections" docx

... graph.Graph analysis of left and right hepatic lobe volumes during the course of the treatmentFigure 3Graph analysis of left and right hepatic lobe volumes during the course of the treatment. Note ... to achieve the goal of increasingthe volume of the FLR [2]. First reported by Makuuchi etal. [3], the aim of PVE is to bring about atrophy of the seg-ments to be resected and induce a compensatory ... patients withmetastatic liver tumors [3-9]. Induction of hypertrophy of the nondiseased portion of the liver reduces the risk of hepatic insufficiency and associated complications afterresection. Clinically...

Báo cáo khoa học: "Minute ampullary carcinoid tumor with lymph node metastases: a case report and review of literature" docx

... the existence of metastases as well as that of regional infiltration of the tumor.ConsentWritten informed consent was obtained from the patient for publication of this case report and the accompanyingimages. ... presentation and literature review sec-tions of this manuscript. KF performed the operation,conceived of this case report, and helped to draft the man-uscript. SS, MY performed the operation and postopera-tive ... (Figure 4-e), and the Ki-67 labelingindex of the tumor cells determined with MIB-1 was 3.2%(Figure 4-f) and overexpression of p53 was not detected.According to the classification of neuroendocrine...

báo cáo khoa học: "The relationship between baseline Organizational Readiness to Change Assessment subscale scores and implementation of hepatitis prevention services in substance use disorders treatment clinics: a case study" pdf

... provide: testing for hep-atitis B and C and immunities to hepatitis A and B; com-prehensive patient education on hepatitis infections and liver health; hepatitis A and B immunizations; and expe-dited ... strength and extent of evidence for clinical practice changes [21];quality of the organizational context [22]; and capacity for internal facilitation [23]. The evidence scale consists of four ... C.Routine testing of all new patients for hepatitis B.Routine testing of all new patients for immunity to hep-atitis A.Routine testing of all new patients for immunity to hep-atitis B.Standardized...

Báo cáo khoa hoc:" Small cell carcinoma arising in Barrett''''s esophagus: a case report and review of the literature" pptx

... management of the patient and to criticalreview of the manuscript. KF contributed to organising and drafting of the manuscript, and critically revised themanuscript. SK assisted in the operation and ... acquisition of data, drafting and writing of the manuscript. AB carried out the histopatho-logic evaluation and contributed to writing of the manu-script. PP contributed to the preoperative and postoperative ... toorganising and drafting of the manuscript, and criticallyrevised the manuscript. AL contributed to manuscriptconception, research, acquisition of data, drafting and writing of the manuscript....

báo cáo khoa học: " The buccal minor salivary glands as starting point for a metastasizing adenocarcinoma – report of a case" pptx

... (CM): Axial image of the head and neck: Tumour (4 × 5 cm2) of the left buccal soft tissues with central necrotic and partly calcified components and resorption of the left mandibleFigure 3Computerized ... (CM): Axial image of the head and neck: Tumour (4 × 5 cm2) of the left buccal soft tissues with central necrotic and partly calcified components and resorption of the left mandible.Chest: ... Torsten Eugen Reichert1 and Oliver Driemel1Address: 1Department of Oral and Maxillofacial Surgery, Regensburg University, Germany, 2Department of Oral and Maxillofacial Surgery, Muenster...

Báo cáo khoa học: "Tumor slices as a model to evaluate doxorubicin in vitro treatment and expression of trios of genes PRSS11, MTSS1, CLPTM1 and PRSS11, MTSS1, SMYD2 in canine mammary gland cancer" pptx

... adjuvantchemotherapy and offers the advantage of an increasedlikelihood of breast conservation. Many drug regimenshave been used for a varied number of cycles and two of the most used drugs, doxorubicin and ... conditions consisted of denaturation at95°C for 15 s followed by 40 cycles for annealing at 60°C for 60 s, and extension at 72°C for 60 s.Relative expression of the genes of interest was calculatedbased ... [5,6], and immunohistochemical detection of Bcl2, p53 and cytokeratins, in human and canine tumors and corre-sponding adjacent tissues, have been similar [8].In dogs, standard treatment for mammary...

Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf

... presence of ADP and Mg2+ and followed by nondenaturing PAGE, the bands of theenzyme and the reactivase were markedly reducedin density, and two bands of the enzymeÆreactivasecomplexes (upper and ... Positions of DD and its subun-its (aD, bD and cD) and the reactivase (DD-R) and its subunits (aR and bR) are indicated on the right of the gels. In (C), to improve visi-bility of the bands of ... vi of theenzymeÆreactivase complexes comprised all of thesubunits from the enzyme (a, b, and c) and the reacti-vase (a and b) (Fig. 5B,C, lanes i and vi). Subunitcompositions of the bands...

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