Báo cáo khoa học: " Development of a fluorescent quantitative real-time polymerase chain reaction assay for the detection of Goose parvovirus in vivo" ppsx

... signal, suggestingthat the established FQ-PCR assay was highly specific (Fig-ure 2). The intraassay and interassay CV of this established FQ-PCR was in the range of 0.8-3% for most of the dynamicrange ... strain of GPV, wasobtained from Key Laboratory of Animal Diseases andHuman Health of Sichuan Province.Aleutian disease virus (ADV), canine parvovirus (CPV),porcine parvovirus, (PPV), Newcastle ... in Sichuan Agricultural University, China. Animalswere bred and maintained in an accredited facility at the Institute of Poultry Sciences in Sichuan Agricultural Uni-versity (Sichuan, China),...

Báo cáo y học: " A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control" docx

... concentration of IC in the HCV RNA duplex real-time RT-PCR assay. Intrinsic performance of the duplex real-time RT-PCR assay LinearityLinearity of the duplex real-time RT-PCR assay wasdetermined ... cost of the duplex real-time RT-PCR assay was considerably lower than that of the CAP/CTM assay, and hence, the former assay ismore suitable for large-scale use.Conclusions The duplex real-time ... WHO Standardwas tested in a batch of 4 replicates in 6 separate runs, i.e. for each dilution, a total of 24 replicates were tested.Linearity of the duplex real-time RT-PCR assay wasdetermined...

Báo cáo khoa học: Alternative splicing produces an H-protein with better substrate properties for the P-protein of glycine decarboxylase doc

... (1988)Resolution and characterization of the glycine cleavage reaction in pea leaf mitochondria. Properties of the forward reaction catalysed by glycine decarboxylaseand serine hydroxymethyltransferase. ... were calculated by nonlin-ear regression analysis using the software packagegraphpad prism (GraphPad Software, San Diego, CA,USA). All kinetic data are means ± SD from three or fouranalyses ... the alternative H-proteins, both the naturallyoccuring FtGLDHAAand the artiﬁcial AtGLDH1AA,are indeed exclusively due to the insertion of twoadditional alanyl residues into the N-terminus...

Báo cáo khoa học: Pyruvate:ferredoxin oxidoreductase and bifunctional aldehyde–alcohol dehydrogenase are essential for energy metabolism under oxidative stress in Entamoeba histolytica pdf

... presence of two catalyticallyindependent domains, the N-terminal domain, display-ing ALDH activity, and the C-terminal domain, con-taining an iron-binding domain, which is involved in ADH activity. ... production in the parasite, withminor and transient contributions of the acetyl-CoA–acetate pathway.Our results also indicated that PFOR and the ALDH activity of EhADH2 were the main targets of ROS ... the extract was always subtracted. Completeinhibition of the ADH activity with pyrazole was deter-mined separately in the ADH assay. Acetyl-CoA synthetaseactivity was determined by following...

Báo cáo hóa học: " Fowlpox virus recombinants expressing HPV-16 E6 and E7 oncogenes for the therapy of cervical carcinoma elicit humoral and cell-mediated responses in rabbits" pot

... and p105Rb, leading to transfor-mation and carcinogenesis [2], facilitate cell immortalisa-tion in primary human keratinocytes [3], increasegenomic instability [4], and maintain the transformedphenotype ... Department of Medical Pharmacology, Università di Milano, Milan, ItalyFull list of author information is available at the end of the articleRadaelli et al. Journal of Translational Medicine ... 55°C, and washed again. The amplifier reagent was then added, and the samplesincubated for 1 h at 55°C; after further washing, this wasreplaced by the label probe reagent for 1 h at 50°C. Afterwashing,...

báo cáo hóa học:" Two levels above and one level below pedicle screw fixation for the treatment of unstable thoracolumbar fracture with partial or intact neurology" pot

... manuscript and designing of data and revising it critically, KCN has contributed in acquisition of data and analysis and interpretation of data, and JHY has contributed in acquisition and analysis of ... contributed in con-ception and design of data, drafting the manuscript andgiven the final approval of manuscript, IWS has contrib-uted in drafting the manuscript and data analysis, HSY hascontributed ... hascontributed acquisition of data and revising it critically,JHH has contributed in acquisition of data, revising the manuscript critically and given the final approval, HKKhas contributed in drafting the...

Báo cáo y học: " Bronchoalveoloar lavage fluid cytokines and chemokines as markers and predictors for the outcome of interstitial lung disease in systemic sclerosis patients" pps

... BAL cell differen-tiations and provided these data for further analyses. GBurmester discussed the data with the last author and madeintellectual contributions. G Riemekasten, as the last andresponsible ... according to the dilution and BALF recovery.StatisticsGraphPad Prism Version 3.02 (GraphPad Software, SanDiego, CA, USA) for Microsoft®, Windows, was used for sta-tistical analysis. The nonparametric ... heter-ogeneity of the control group. In conclusion, we identified several abnormalities in the cytokine and chemokine patterns in BALF of SSc patients,suggesting an important role of these mediators in the...

Tài liệu Báo cáo khoa học: Development of a new method for isolation and long-term culture of organ-speciﬁc blood vascular and lymphatic endothelial cells of the mouse pdf

... the tsA58T Ag cDNA carry-ing the A4 38V mutation were PCR-ampliﬁed from COS-7cDNAs using the following primers: LTA-1F, 5¢-CTCGAGATGGATAAAGTTTTAAACAGAG-3¢ and LTA-1R, 5¢-TGAAGGCAAATCTCTGGAC-3¢ ... lymphaticendothelial cells of the mouseTakashi Yamaguchi, Taeko Ichise, Osamu Iwata, Akiko Hori, Tomomi Adachi, Masaru Nakamura,Nobuaki Yoshida and Hirotake IchiseLaboratory of Gene Expression and Regulation, ... (data not shown), indicatingthat the cells maintained the physiological characteristic of acetylated-LDL uptake.Lyve-1-positive liver sinusoidal endothelial cells in vitroIntriguingly, almost...

Báo cáo khoa học: "Development and Evaluation of a Broad-Coverage Probabilistic Grammar of English-Language Computer Manuals" pdf

... nonterminal labels of the treebank grammar. For example, our grammar main- tains a fairly large number of semantic classes of singular nouns, and it is natural to stipulate that each of them ... in the mnemonic tree), rather that in terms of the actual nonterminals of the grammar. It is in this manner that we can obtain efficient and reliable estimates of our parameters. Since the ... accuracy can be ascertained. The value of a large bracketed training corpus is that it allows the grammar- ian to obtain quickly a very large 3 set of sentences that 2Actually there are 18 x...

Báo cáo khoa học: "Development of a Stemming Algorithm" pdf

... means of associating re- lated items of information, as they are in an automated library catalogue, and where the catalogue can be in- terrogated in an on-line mode, it seems best to use a strong ... University of Chicago, Department of Lin- guistics. variety of applications are considered in evaluating the theoretical and practical attributes of several previous algorithms. As a major part of ... word of its derivational and inflectional suffixes. Researchers in many areas of computational linguistics and information retrieval find this a desirable step, but for varying reasons. In auto-...

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