báo cáo khoa học: " The YlmG protein has a conserved function related to the distribution of nucleoids in chloroplasts and cyanobacteria" pdf

... 5'-CACCGAGAAGTCAA-CAGCTCGGTCATCGAC-3' and 5'-TCAAGTCTTC-CAATTTCTACCCAGTGCTGC-3' for AtYLMG1-1, 5'-CCTCAACATATATAACACCATC-3' and 5'-GACAG-GTTCAGGTCATAGAAG-3' ... 5'-GACAG-GTTCAGGTCATAGAAG-3' for At5g21920, 5'-TATCT-GAACACTCCGTTGACGGTA-3' and 5'-CAAAGATAAACGGAATACGATC-3' for At4g27990, 5'-GCAATGG-GAAGCAGTGGTGG-3' and 5'-GGGAGAAGAGACG-GGTTTCG-3' ... in the FOX lines, the insertion was amplified by PCR using primers 5'-GTACGTATTTTTACAACAATTACCAACAAC-3' and 5'-GGATTCAATCTTAAGAAACTTTATTGCCAA-3', and then sequenced by a primer...

Báo cáo khoa học: Odorant binding protein has the biochemical properties of a scavenger for 4-hydroxy-2-nonenal in mammalian nasal mucosa doc

... properties of this form (in terms of aggregation state and bindingwith different ligands including AMA, HNE and unde-canal) are analogous to those of the native form (datanot shown). The cytotoxic ... incubation with a rabbit serum raised against HNE protein adducts. Ligand-binding tests showing the functionality of the same HNE-treated porcine (B) and bovine (C) OBPs as in the immunostaining. ... with a cytotoxic amount (20 lm) of HNE in the absence and presence of a molar excess of bovine OBP (40 lm). The protein was maintained in the extracellular environment by immobilization to a Ni-NTA...

Báo cáo khoa học: Serine-arginine protein kinases: a small protein kinase family with a large cellular presence potx

... signiﬁcance of the ‘spacer domain’for the functional properties and the particular regula-tion of each kinase.l The extra- and intracellular signals that regulateSRPK function. Further investigations ... picture of SRPK function and importance. TcSRPK, the SR protein kinase of a protozoan, the parasite Trypanosoma cruzi,which displays trans- and cis-splicing and was cloned and characterized in 2003, ... respiratory factor-1; PGC-1, peroxisomeproliferator activated receptor c coactivator-1; RS, arginine-serine; SAFB, scaffold attachment factor B; SR, serine-arginine;SRPK, serine-arginine protein kinase.570...

Báo cáo khoa học: "Who, What, When, Where, Why? Comparing Multiple Approaches to the Cross-Lingual 5W Task" pptx

... Association for Computational Linguistics (ACL-02), Philadelphia, PA, USA. Mary Harper and Zhongqiang Huang. 2009. Chinese Statistical Parsing, chapter to appear. Aria Haghighi, Kristina Toutanova, ... seeks to get at the understandability of the MT output, rather than just n-gram overlap. Translation exacerbates the problem of auto-matically evaluating 5W systems. Since transla-tion introduces ... Cross-Lingual 5W Task In the cross-lingual 5W task, a system is given a sentence in the source language and asked to produce the 5W’s in the target language. In this task, both machine translation...

Báo cáo khóa học: Mycoplasma pneumoniae HPr kinase/phosphorylase Assigning functional roles to the P-loop and the HPr kinase/phosphorylase signature sequence motif doc

... 4 and 6 min after the start of the reaction, 20 lLmixturewastransferredtoafreshreactiontubeandthereactionwas stopped (96 °C, 4 min). The fractions were then separated on a native polyacrylamide ... R204K, and G20 7A mutant proteins. In contrast, the apparent Kdvaluesfor ATP were increased even more for the G15 4A and the S16 1A proteins (Table 1). These two proteins exhibitreduced kinase activity ... Northborough, MA, USA). Protein concentrationwas determined by the method of Bradford [25] using the Bio-rad dye-binding assay with BSA as standard.Assay of HPrK/P activityActivity assays were carried...

Báo cáo khoa học: Heavy metal ions inhibit molybdoenzyme activity by binding to the dithiolene moiety of molybdopterin in Escherichia coli pdf

... form of MPT, and not to other metal-substituted forms of MPT. The chemical and physicalsimilarities of tungstate and molybdate are a result of their equal atomic and ionic radii and similar electro-negativity ... by the MogA and MoeA proteins. The insertion of copper, cadmium and arsenite is independent of the MogA and MoeA pro-teins. Even if the copper-containing MPT–AMP inter-mediate is formed in ... transport. Res Microbiol 152, 311–321.17 Tomatsu H, Takano J, Takahashi H, Watanabe-Takah-ashi A, Shibagaki N & Fujiwara T (2007) An Arabidop-sis thaliana high-afﬁnity molybdate transporter...

Báo cáo khoa học: Etoposide upregulates Bax-enhancing tumour necrosis factor-related apoptosis inducing ligand-mediated apoptosis in the human hepatocellular carcinoma cell line QGY-7703 pdf

... Smac/DIABLO promotes the activation of caspases, such as procaspase-9 and caspase-3,by binding to the inhibitor of apoptosis proteins and thusdisrupts linkage of the caspase–inhibitor of apoptosisproteins ... eﬀector caspases, such as caspase-3 and -7[55]. The death ligands and the chemotherapeutic agentsare two distinct classes of signals used to induce apoptosis and activate the caspase cascade. ... asFas-associated death domain protein (FADD) [15] and death-associated protein 3 (DAP-3) [16] to activate initiatorcaspase-8 and subsequently the eﬀector caspases leading to apoptosis [6,7]. In contrast,...

Báo cáo khoa học: Glycolipids with nonreducing end a-mannosyl residues that have the potential to activate invariant Va19 NKT cells pptx

... ELISA. The TCR structure of the hybridomas is listed on the left. NB116 and NB202 have an amino acid variation at the CDR3region of the invariant Va19-Ja33 a chain, whereas the others have a ‘canonical’ ... Shimamura1, Yi-Ying Huang1, Naoki Okamoto1, Yutaka Watanabe2, Yoshiko Murakami3,Taroh Kinoshita3, Yoshio Hirabayashi4, Chikara Murakata4, Yukishige Ito4 and Tomoya Ogawa41 ... observed. For instance, hybridomas expressing TCRsconsisting of an a chain with a variation in the Va19-Ja33 junction and a Vb6+b chain showed afﬁnity towards a- mannosyl ceramide and a- Man-GlcNH2-PtdIns,...

báo cáo khoa học: "Thick primary melanoma has a heterogeneous tumor biology: an institutional series" pot

... in data analysis and interpretation, manuscript drafting and final approval CYparticipated in data acquisition and analysis, manuscript drafting and finalapproval; BL participated in data acquisition ... acquisition and analysis, manuscriptdrafting and final approval; GW participated in study design, data analysis and interpretation, manuscript drafting and final approval; JMK participated in study ... status and ulceration as individual vari-ables, we incorporated those factors into the patients’AJCC stage as a way to provide more accurate, clinicallyrelevant survival information for the...

Báo cáo khoa học: "Experience with adjuvant chemotherapy for pseudomyxoma peritonei secondary to mucinous adenocarcinoma of the appendix with oxaliplatin/fluorouracil/leucovorin (FOLFOX4)" docx

... peri-tonei secondary to appendiceal mucinous adenocarci-noma and review the literature.Case presentation A 55-year-old male patient had suffered from acute onset of abdominal pain and abdominal ... replace serial de-bulking as the standard of care for patients with peritonealspread of appendiceal epithelial neoplasms [20]. PMCAbehaves like peritoneal carcinomatosis from the originalcolorectal ... postoperative care and revised the manuscript. WYK assessed the histologicalspecimens and prepared the histological slides. JSHreviewed the clinical data and helped to draft and revise the manuscript....

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