báo cáo khoa học: " Origins of the amphiploid species Brassica napus L investigated by chloroplast and nuclear molecular markers" pps

báo cáo khoa học: " Origins of the amphiploid species Brassica napus L. investigated by chloroplast and nuclear molecular markers" pps

... King, Origins of the amphiploid species Brassica napus L. investigated by chloroplast and nuclear molecular markers BMC Plant Biology 2010, 10:54Additional file 1 Table S1. Details of the samples ... species Brassica napus L. investigated by chloroplast and nuclear molecular markersCharlotte J Allender*1 and Graham J King2AbstractBackground: The amphiploid species Brassica napus (oilseed ... understanding of the mechanisms and genetic factors controlling the creation of stable amphiploids, and this will facilitate the incorpo-ration of novel alleles from the wider Brassica genepool.Authors'...

Báo cáo khoa học: Characterization of the Drosophila Methoprene -tolerant gene product Juvenile hormone binding and ligand-dependent gene regulation potx

... Invitrogen). The cells were see-ded at a density of 2.5 · 105cells per well of 24-well platesone day before transfection. 2 lL of lipofectin (Invitrogen)per each well was mixed with 25 lL of SFM and ... another 15 min. Then, this wasmixed with 200 lL of SFM and overlaid onto S2 cells ineach well. This was followed by 5 h incubation at 27 °C, and the transfection mixture was replaced by 250 lLofSFM ... the nucleus. In theseexperiments the use of cultured cells allows for com-plete depletion of JH. These observations are consis-tent with the previous report in vivo [29] and rule out the ligand-dependent...

Báo cáo khoa học: Shedding of the amyloid precursor protein-like protein APLP2 by disintegrin-metalloproteinases Retinoic acid-induced upregulation of substrate and proteinase ADAM10 during neuronal cell differentiation ppt

... [(N-benzyloxycarbonyl-val-leu-leu-leucinal)Z-VLL-CHO] on APLP2 shedding in the human astro-glioma cells U373. These cells overexpress humanwild-type APP and therefore allow detection of BACE-1-generated ... celllines. We cannot completely rule out the possibilitythat the increase in secretion of soluble APLP2 follow-ing treatment with RA may also be due to the increasein the amount of APLP2 and ... differentiation of neuroblastoma cells. Reti-noic acid treatment of two neuroblastoma cell lines upregulated the expres-sion of both APLP2 and ADAM10, thus leading to an increased release of soluble APLP2.AbbreviationsADAM,...

Báo cáo khoa học: "Assessment of the California mastitis test usage in smallholder dairy herds and risk of violative antimicrobial residues" pptx

... analysis of variance, using the general linearmodel procedure. The model included the effects of the animal, the quarter, the stage of lactation (1-12 months), the bacteriological findings and ... disposable culturing loop(10 l) was dipped into the milk sample and a total of 6-8lines made in one agar section by turning the loop oncebetween the streaking lines. Each quarter milk sample(10 ... from lactating cows in the Rift Valley of Kenya and tested using the CMT, somatic cell counts (SCC) and bacterial culture. The samples were also screened forviolative drug residues using the...

báo cáo khoa học: "Characterization of the stress associated microRNAs in Glycine max by deep sequencing" pot

... Computational identification of plant microRNAs and their targets, including a stress-induced miRNA. Mol Cell 2004, 14(6):787-799. 8. Phillips JR, Dalmay T, Bartels D: The role of small RNAs in ... Table 3 file 7. The energy scope of these miRNAs ranged from 70.8 kcal/mol (Gma-050) to -24.2 kcal/mol (Gma-013). The expression levels of these candidates ranged broadly, from thousands of ... losses worldwide, unraveling the complex mechanisms underlying stress resistance of plants has profound significance. Recently, the newly developed sequencing technologies, such as the Illumina...

Báo cáo khoa học: Origins of DNA replication in the three domains of life pdf

... manifest itself both at the level of the immediate chromatin environment of the intergenicregion, and also at the level of topological status of the DNA as a result of transcription of the adjacent ... features of a number of archaeal origins of replication and thishas allowed the prediction of the localization of repli-cation origins in a diverse range of archaea. Interest-ingly, the second Sulfolobus ... learnt about the nature of initiators and origins of replication, particularly in simple modelsystems. However, many of the molecular details of the basis of origin selection remain poorly understood,particularly...

Báo cáo khoa học: "Distribution of the putative virulence factor encoding gene sheta in Staphylococcus hyicus strains of various origins" pps

... exhC, and exhD by multiplex PCR revealed the presence of exhD in 17 of the S. hyicus strains isolated in Russia and the genes exhC and exhD for one and two S. hyicus strains, respectively, isolated ... internationally available, the role SHETB plays in exudative epidermitis remains unclear. According to Sato et al. [8] SHETA is under chromosomal control, and SHETB is plasmid-controlled. Strains investigated ... aureus and S. hyicus, and also for SHETB. However, according to the findings of Sato et al. [8] and the results of the present study, SHETA seems to contribute to the clinical signs of exudative...

Báo cáo khoa học: " Effect of the medical emergency team on long-term mortality following major surgery"

... noted. Allpatients treated in our hospital suffered at least loss of one rel-ative, for example, a partner or child, and two mothers of ourcohort lost both of their children. The majority of patients ... psychoemotional aftermath, the full impact of the wave on the mental health of the survivors isstill unknown [2]. In February 2005 the World Health Organi-zation, among others, estimated that up to 50% of ... cohort grew multi-resistantAcinetobacter, E. faecium, mould and Candida species whileLimchawalit and Suchato [47] described Aeromonas species, Klebsiella, E. coli and Proteus mirabilis. These pathogenswere...

Tài liệu Báo cáo khoa học: Motion of the Ca2+ -pump captured ppt

... concentrationgradient. The importance of the SR Ca2+-pump wasrealized in the early 1960s by Ebashi and Lipmann[5,6] and, since then, most of the molecular compo-nents in the regulation of skeletal muscle contractionhave ... visualizedconformational changes in the sarcoplasmic reticulum Ca2+-ATPase(SERCA) in real time at the single-molecule level. The analyses of individ-ual SERCA molecules in the presence of ... in the range 10 nM to 100 lM. (B, C) Typicaldistributions of the lifetime of the elevated conformations of SERCA in the presence of 100 l M free Ca2+ and 10 nM (B) and 100 l M ATP (C). The...

Tài liệu Báo cáo khoa học: Role of the cag-pathogenicity island encoded type IV secretion system in Helicobacter pylori pathogenesis pptx

... multiple pathways to trigger host-cell motility and elongation (15) and the onset of mitogenic genes and cell proliferation (16), aswell as inhibit apoptosis (17). The interplay of each of these ... transmembranedomains with the mid domain (also called the repeatdomain) exposed to the extracellular space [10]. Thus,it is still not fully clear whether the H. pylori T4SSpilus is more similar to that of Agrobacterium, ... T4SS then triggers the secretion of substrates from the bacterial cytoplasm directly into the cytoplasm of infected host cells or into the extracellular milieu. (B) Model-1 for the assembled T4SS...

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