... extended conformation, with its phosphate moiety located in the proximity of the zincion and with the hydroxymethylene group pointingtoward Pro295. DHAP interacts with the catalyticallyessential ... hydroxyl group onto the substrate, as observedin other members of the amidohydrolase superfamily[24,25]. However, as no structure of complexes with either the substrate, product or inhibitors had beenreported, ... 3B). Its guanidinium group contacts both the phosphate and the hydroxyl moieties present onDHAP, and appears to be a major contributor to ACMS recognition by stabilizing interactions with the negatively...