báo cáo khoa học: " Deep sequencing identifies novel and conserved microRNAs in peanuts (Arachis hypogaea L )" pps
báo cáo khoa học: " Deep sequencing identifies novel and conserved microRNAs in peanuts (Arachis hypogaea L.)" pps
... regulatoryroles in plants. Annual Review of Plant Biology 2006, 57:19-53.9. Zhang BH, Wang QL, Pan XP: MicroRNAs and their regulatory roles in animals and plants. Journal of Cellular Physiology ... DCL1 isa key enzyme in miRNA biogenesis and mutating thisgene results in globally decreased miRNA levels in plants, ultimately resulting in pleiotropic defects [16,17].* Correspondence: xingjunw@hotmail.com† ... article as: Zhao et al.: Deep sequencing identifies novel and conserved microRNAs in peanuts (Arachis hypogaea L. ). BMC PlantBiology 2010 10:3.Submit your next manuscript to BioMed Centraland...
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Báo cáo khoa học: "Clinical implications of novel activating EGFR mutations in malignant peritoneal mesothelioma" ppt
... proteinpreparation and Western blot analysis was performed fol-lowing the methods described previously.Equal amounts of protein were prepared in 50 μlofLaemmli loading buffer andresolvedusing4-15%Figure ... warranted. In summary, we have identifi ed novel activating EGFRmutations in MPM associated with optimal resectability and prolonged survival. Clinically these mutations mayultimately have utility in ... Rockford, IL).Nitrocellulose membranes were incubated overnightandprobedwithanti-phospho-EGFR (Cell SignalingTechnology®, Danvers, MA), and EGFR antibody (CellSignaling Technology). The densities...
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Báo cáo khoa học: "Identification of a novel germ-line mutation in the TP53 gene in a Mexican family with Li-Fraumeni syndrome" doc
... ampi-cillin. Twelve colonies were picked and grown in LuriaBertani media in the presence of ampicillin. Plasmidextraction was performed by the alkaline lysis method and purified for sequencing. ResultsTP53 ... reported asinvolved in several other alterations, including deletions and insertions of one or several nucleotides. In 1994,Birch et al [3] reported a complex mutation involvingdeletion of 11 ... clinical evaluation and genetic coun-seling of the family and collected data; OG-H, CT-B, and EP-C purified the DNA, performed PCR amplifications,DHPLC, and PCR products sequencing; AC-B and...
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Tài liệu Báo cáo khoa học: "Applications of GPC Rules and Character Structures in Games for Learning Chinese Characters" doc
... sublexical information in Chinese. The games aim at implicitly informing players of the Chinese GPC rules, mimicking the process of how infants would apply statistical learning (Saffran et al., ... read-ing ability, e.g., (Siok and Fletcher, 2001). Moreo-ver, the ability to detect and apply phonological consistency in GPCs, including the roles of PCs in PSCs in Chinese, plays an instrumental ... J. C. Ziegler and U. Goswami. 2005. Reading acquisition, developmental dyslexia, and skilled reading across lan-guages: A psycholinguistic grain size theory, Psychological Bulletin, 131(1),...
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Tài liệu Báo cáo khoa học: "Automatic Identification of Pro and Con Reasons in Online Reviews" ppt
... time-consuming and costly task. In this paper, we pro-pose a framework for automatically identifying reasons in online reviews and introduce a novel technique to automatically label training data ... them and Section 4 explains our automatic data labeling process. Section 5 describes experimental and results and finally, in Section 6, we conclude with future work. 2 Pros and Cons in Online ... sentence level, and document level. Word level opinion analysis includes word sentiment classification, which views single lexi-cal items (such as good or bad) as sentiment car-riers, allowing...
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Báo cáo khoa học: Roles of the SH2 and SH3 domains in the regulation of neuronal Src kinase functions pptx
... coefficients(http://www.expasy.org).Immunoblotting and in vitro kinase activity assayProteins purified from BL21(DE3) cells were subjected toSDS ⁄ PAGE and Western blotting. Antibodies includinganti-Src IgG (Millipore, Billerica, ... clamp recordings in HEK-293 cells have been described previously [17,29]. In brief, cells were bathed in a standard extracellular solutioncontaining NaCl (140 mm), CsCl (5 mm), CaCl2(1.2 mm),HEPES ... its ligand. D99N and R175K mutationstherefore inhibit interactions with ligands of the SH3 and SH2 domains, respectively, both intra- and inter-molecularly, and thereby disrupt the overall functionsof...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
... (a) Class Iincludes polyhydric alcohols (sorbitol, glycerol, xylitol,adonitol, mannitol) and amino acids and derivatives(glycine, alanine, proline, serine, lysine, b-alanine and taurine) that ... d-stachyose, glycine, l- alanine, l- proline, l- lysine, l- serine, b-alanine, taurine, sarcosine,dimethylglycine, glycine betaine, trimethylamine N-oxide, and cytidine 2¢-3¢ cyclic monophosphate were also ... Trimethylamine-N-oxide-induced folding of a-synuclein. FEBS Lett 509,31–35.6 Meng F, Park Y & Zhou H (2001) Role of proline,glycerol, and heparin as protein folding aids duringrefolding of rabbit muscle...
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Báo cáo khoa học: Structural origins for selectivity and specificity in an engineered bacterial repressor–inducer pair pdf
... theorigins of selectivity and specificity. In all crystal structures solved to date,the ligand-binding position, as well as the positioning of the residues liningthe binding site, is extremely well ... arereplaced by Leu and Ile, water molecules are attachedto the serines in all other TetR structures and fill acleft between the ligand and the protein. Close inspec-tion of these water molecules ... resolution shell.bAccording to PROCHECK [27].Table 2. Superposition of the ligand-binding sites and ligand positions in selected TetR complexes.No ligandTetR(K64 L 135I138)IaNo ligandTetR(K64 L 135I138)II4-ddma-atcTetR(K64 L 135I138)Ia4-ddma-atcTetR(K64 L 135I138)IIatc...
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Báo cáo khoa học: Differential expression of liver and kidney proteins in a mouse model for primary hyperoxaluria type I pdf
... catalase(Cat -L) , enolase (Eno1 -L) , alanine-glyoxylateaminotransferase (Agt -L) , actin (Actin -L) and glyoxylate reductase ⁄ hydroxypyruvatereductase (Grhpr -L) from liver. (B) Westernblot analyses of enolase ... capillar-ies were equilibrated for 15 min in reducing buffer contain-ing 50 mm Tris ⁄ HCl, pH 8.8, 30% glycerol, 6 m urea, 2%SDS and 1% dithiothreitol, followed by a blocking step in similar ... subsequentidentification by MS.MALDI-TOF MSProtein spots were manually excised from Coomassie-stained gels, and tryptic in- gel digestion and desalting stepswere performed using 96-well ZipPlates (Millipore, Bedford,MA,...
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Báo cáo khoa học: Role of glutaredoxin 2 and cytosolic thioredoxins in cysteinyl-based redox modification of the 20S proteasome docx
... containing 500 mmNaCl plus 1 lL protease inhibitor cocktail (Calbiochem) per20 mg cellular pellet. After 10 cycles of 2 min vortexing and 1 min resting on ice, the cell lysate was centrifuged ... stimulated by car-diolipin (Fig. 6A, lanes 3 and 2, respectively, and Fig. 6B). In parallel, deglutathionylation by Grx2(evaluated by anti-GSH blotting analysis) in the pres-ence of cardiolipin ... purification, samples (30 lg) were dissolved in gelloading buffer containing 10 mM N-ethylmaleimide and applied toSDS–PAGE. (A) Representative blots of natively (n-PT) and in vitroS-glutathionylated...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt namNghiên cứu tổ chức pha chế, đánh giá chất lượng thuốc tiêm truyền trong điều kiện dã ngoạiNghiên cứu tổ hợp chất chỉ điểm sinh học vWF, VCAM 1, MCP 1, d dimer trong chẩn đoán và tiên lượng nhồi máu não cấpGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longPhát hiện xâm nhập dựa trên thuật toán k meansNghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chếTìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roNguyên tắc phân hóa trách nhiệm hình sự đối với người dưới 18 tuổi phạm tội trong pháp luật hình sự Việt Nam (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtTrách nhiệm của người sử dụng lao động đối với lao động nữ theo pháp luật lao động Việt Nam từ thực tiễn các khu công nghiệp tại thành phố Hồ Chí Minh (Luận văn thạc sĩ)MÔN TRUYỀN THÔNG MARKETING TÍCH HỢP