báo cáo khoa học: " Ascorbate metabolism and the developmental demand for tartaric and oxalic acids in ripening grape berries" pot
báo cáo khoa học: " Ascorbate metabolism and the developmental demand for tartaric and oxalic acids in ripening grape berries" pot
... for citation purposes)BMC Plant BiologyOpen AccessResearch article Ascorbate metabolism and the developmental demand for tartaric and oxalic acids in ripening grape berriesVanessa J Melino1,3, ... insights into Asc metabolism in wine grapes (Vitis vinifera c.v. Shiraz.) and thus ascertainwhether the developmental demand for TA and OA synthesis influences Asc accumulation in the berry.Results: ... the geneencoding the Asc recycling enzyme dehydroascorbatereductase, resulted in a two to four-fold increase in ascor-bic acid levels and a significant increase in the redox stateof the ascorbate...
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... probably induced by streng-thening of the interaction between the structural TnC-binding site and the C-domain of TnC accompanyingCa2+binding to site IV of TnC. In vertebrate tropo-nin, the ... 116–131). In the relaxed state, the inhibitory region bindsto actin and inhibits actin–myosin interaction [11,12],while in the contractile state, Ca2+-binding to site I and ⁄ or II of TnC causes the ... on the surface of the N-domain [15], result-ing in hydrophobic interaction between the N-domain and the regulatory TnC-binding site [16]. This inter-action induces the dissociation of the inhibitory...
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Báo cáo khoa học: Definition of the residues required for the interaction between glycine-extended gastrin and transferrin in vitro pptx
... [18]. The biochemical basis of the gastrin–transferrin inter-action is still unknown. Knowledge of the regions oftransferrin required for the binding of gastrin, and of the regions in gastrin ... explorewhether Ggly interacts with transferrin in vitro,toinvestigate whether iron is required for the Ggly–trans-ferrin interaction, to define the domains ⁄ residues ofGgly involved in the interaction ... gastrin binding, and that the observed binding was to the unmutated lobe. The observation that the extent of crosslinking was the same for Mono N and Mono C transferrin as for wild-type ApoTf (Fig....
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Báo cáo khoa học: UXT interacts with the transcriptional repressor protein EVI1 and suppresses cell transformation ppt
... (Clontech)were transformed with the combination ofplasmids shown. The growth of single yeastcolonies containing these plasmids areshown in (B) and (E) on SD lackingL-histi-dine and adenine and (C) on ... were transformed with the combination of plasmids shown. The growth of single yeast colonies containingthese plasmids are shown in (B) and (E) onSD plusL-histidine and adenine and (C) and (F) ... unable tobind UXT (Fig. 5D–F, quadrant 2) but retains the ability to bind mCtBP2 (Fig. 5D–F, quadrant 5). The transforming activity of Evi1 containing the UXT-binding mutant Rp domain was investigated...
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Báo cáo khoa học: Procarboxypeptidase A from the insect pest Helicoverpa armigera and its derived enzyme potx
... provides the a-mating factor signal for secretion of the recombinantprotein.Transformation and selection of the productive clonesPrior to the transformation the vector was linearized withSacI. The ... proteolyticactivation; substrate specificity; protein inhibitor. The understanding of the digestive process in pest insectsis a key step in the design of many insecticides, includinginsect-resistant transgenic plants ... insect.Two forms of pancreatic-like carboxypeptidases, CPA and CPB, are involved in the degradation of dietaryproteins. The two isoforms of CPA, A1 and A2, differ in specificity with the former having...
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Báo cáo khoa học: Molecular dissection of the biosynthetic relationship between phthiocerol and phthiodiolone dimycocerosates and their critical role in the virulence and permeability of Mycobacterium tuberculosis doc
... DIMs in the cell envelope raises the possibility that thesecompounds act in vivo by interacting with some com-ponent of the host cell and by modulating the hostimmune response to contain the infection. ... postinfection, we observed amarked difference between the number of CFUs of the wild-type strain and that of the PMM56 mutantstrain in lungs and in the spleen (Fig. 7A). Indeed,both strains ... was determined by calculating the ratio ofmutant to wild-type bacteria after correcting for the ratio of these strains in the inoculum. It appearedthat, 21 days after infection, the ratio of...
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Báo cáo khoa học: Thyroid hormone induces the expression of 4-1BB and activation of caspases in a thyroid hormone receptor-dependent manner pptx
... modulated during bothearly and late responses.We also examined the expression of TRAF1 and TRAF2because they interact and regulate the function of the 4-1BBproteins to induce NF-jB [18]. In the absence ... 4-1BB-Ligand, it increased the caspase activities. An increase in the caspase activities wasalso observed in the HeLaTR cells transfected with 4-1BBcDNA, and the 4-1BB-Ligand further increased the caspaseactivities ... used as the template for the RT-PCR wasextracted from the subconfluent cultures of the HeLaTR cells that were cultured in the presence of T3 for the indicated time. RT-PCR wasperformed as in (A).Ó...
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Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identification of RGD/KGE sequences as binding motifs for C2 domains pdf
... expressed in Escherichia coli and used in pull-down assays. In theseexperiments, cardosin A binds consistently to the C2domain (Fig. 5, lane 2), indicating therefore that thisdomain of PLDa ... sequences present in integrin-binding molecules because of its exposed loca-tion. On the basis of these structural findings, it washypothesized that the interaction between PLDa and cardosin A may be ... cleavage.DiscussionCardosin A is unique among known plant asparticproteinases in having an RGD motif located at the surface of the protein [4]. The presence of this well-known integrin-binding motif [11], and the...
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Báo cáo khoa học: "Study protocol of the German "Registry for the Detection of Late Sequelae after Radiotherapy in Childhood and " ppsx
... obtained in a few years. The described study proto-col and the documentation forms are open for the use byother study groups. To further increase the number ofpatients in order to obtain faster ... drafted the manu-script. AS, HP, CR, BP, BT, RDK and KD participated in the design of the registry study and are involved in continuingoptimization. NW is the study chairman of RiSK, devel-oped the ... Group.Competing interests The authors declare that they have no competing interests.Authors' contributionsTB is the study coordinator of RiSK, participated in the development of the study trial and...
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báo cáo khoa học: "Population genetics of the metabolically related Adh, Gpdh and Tpi polymorphisms in Drosophila melanogaster : II. Temporal and Spatial Variation in an Orchard Population Karen M. NIELSEN A.A. HOFFMANN S.W. McKECHNIE" potx
... an increase in the Fallele frequency in autumn and winter months (fig. 2). Total monthly rainfall, meandaily maximum and minimum temperature for each month and the ... underlying the large scale latitudinal cline in Australasia. The negative temperature association we observed is therefore in the opposite direc-tion. Also, in the geographical ... potential in detecting and understandingepistasis and the forces which structure the genome.Macrogeographic patterns of variation have been reported for all 3 of thesepolymorphisms...
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