báo cáo khoa học: " Recovery and characterization of a Citrus clementina Hort ex Tan ''''''''Clemenules'''''''' haploid plant selected to establish the reference whole Citrus genome sequence" pot
... 2.3 and 4.6 cm. The max-
imum value of the haploid plants was similar to the leaf
length of the aneuploid plant (4.4 cm).
Histological characterization
The histological structure of anthers of the ... Biology
Open Access
Research article
Recovery and characterization of a Citrus clementina Hort. ex Tan.
'Clemenules' haploid plant...
... ATT CCA TCA TCA TCA
TCA TCA TCA GGG CAC GAC ACA CAT CCC C; and
reverse primer, GAT CGG TAC CTC ATT ACA GAG
GAG GGA TAT GGG GAA C. The PCR reaction was
done as described above. The amplified PCR ... trypsin and the potential glycopeptides were
bound to a ConA column. MALDI-TOF MS analysis
of the eluted material revealed a few peptides, of which
the largest had a molecu...
... was subtracted from
the total uptake to calculate the carrier-mediated uptake. Inset:
Eadie–Hofstee transformation of the specific Gly-Sar uptake data
[S, Gly-Sar concentration (m
M); v, uptake ... incubation periods from 10 min up to 8 h.
The amount of Bip-Pro in the extracellular uptake medium
was quantified according to the laboratory standard HPLC
(La-Chrom
Ò
; Merck-H...
... the P. horikoshii CoADR),
and TG104 (5¢-CGCGCCATGGAAAAGAAAAAGGTA
GTCATAA-3¢) and TG105 (5¢-CGCGGTCGACCTAGAA
CTTCAAAACCCTGGC-3¢) for the P. furiosus CoADR.
The N terminus of the CoADRs were based ... lL. Oxidation
of NAD(P)H was monitored at 340 nm. The standard oxid-
ase assay used to monitor the purification of phCoADR
was conducted with 100 lm NADH and FAD as above....
... was PCR amplified from chromosomal DNA
of P. furiosus using the primers BG1279 (5¢-GCGCG
CCATGGCATCCGAGAAGATGGTTGCTATCA, sense)
and BG1297 (5¢-GCGCG
GGATCCTCATTTAAGCAT
GAAAACAACTTTGCC, antisense), ... the
initial activity of Pf-TDH was checked using butane-
2,3-diol as substrate in the standard oxidation reaction
and acetoin in the reduction reaction. The activity of
Pf-TDH...
... pots, and all plant tissue above ground was
used for the analyses. (A) Total RNA was extracted and 20 lg RNA
was loaded in each lane and blotted as indicated in Experimental
procedures. To prove ... Nagasawa T, Ohkishi H, Kawakami B, Yamano H,
Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-ala-
nine chloride-lyase (deaminating) of Pseudomonas putida
CR 1–1. Purification and...
... (5¢-GTCGTCTTGCGGTCACCT
GGACATGCATCAACGAACAG-3¢) and Ct-Aspf1 (5¢-GT
CGTCTTGGATCCTCTCGAGTCTCAATGAGAACACA
GTCTCAAGTC-3¢). These primers contained BstEII and
BamHI sites and were used to generate a fragment that was
cloned in the same ... proteins as inhibitors: Aspf1, Aspf1D(7–
22), a- sarcin and a- sarcin D(7–22). The inhibitor amount is plotted in
logarithmic scale. The st...
... Ikeda
1
, Shinya Kajita
1
, Masaya Nakamura
2
and Yoshihiro Katayama
1
1
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo,
Japan;
2
Forestry ... large excess of water was added and the resultant
precipitate was collected as DHP-GOU.
Isolation of the fungi and enzyme
Activity of the b-aryl ethe...
... characterization
of a bovine BAC library with four
genome- equivalent coverage
André E
GGEN
a, ∗
, Mathieu G
AUTIER
a
,
Alain B
ILLAUT
d
, Élisabeth P
ETIT
a
, Hélène H
AYES
a
,
Pascal L
AURENT
a
, Catherine ... or
so BAC clones analysed by FISH was chimeric. This BAC library increases the international
genome coverage for cattle to around 28 genome equivalents and e...
... complexes in the phe-
nylpropanoid and flavonoid pathways. Physiol Plant 1999,
107:142-149.
59. Nakatsuka T, Sato K, Takahashi H, Yamamura S, Nishihara M: Clon-
ing and characterization of the ... before anthesis (-2da), anthesis (da), one day after
anthesis (+1da), and three days after anthesis (+3da), and in closed and open stamen (st
c
and st
o
), corm, tepals (pt)...